Eosinophil cationic protein, myeloperoxidase and tryptase in children with asthma and atopic dermatitis

Kristjánsson, Sigurður; Shimizu, Toshio; Strannegård, Inga-Lisa; Wennergren, Göran

doi:10.1111/j.1399-3038.1994.tb00244.x

Cited by 41 publications

(31 citation statements)

References 25 publications

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“…The study by Monteserin et al [65] showed that IgE-dependent mechanism might promote MPO released by neutrophils at allergic sites. In atopic asthmatic children, higher levels of IgE, MPO and ECP were observed [66]. In our study, statistically significant inverse correlation was found between CC16 and IgE in smoking smelters (Table 4).…”

Section: Ijomeh 2006;19(4)supporting

confidence: 64%

Serum Clara Cell Protein as an Indicator of Pulmonary Impairment in Occupational Exposure at Aluminum Foundry

Hałatek¹,

Trzcinka-Ochocka²,

Matczak³

et al. 2006

International Journal of Occupational Medicine and Environmental Health

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Objectives: Although some of the exposures in aluminum (Al) smelting have been well characterized, and respiratory disorders in aluminum production workers are well known, the relationship between internal aluminum loads and appropriate lung biomarkers have not been elucidated. The aim of our work was to carry out a comprehensive investigation in workers employed in the Aluminum Foundry Casting Department with special reference to currently existing hygiene standards, known as threshold limit values (TLV) based on aluminum effects on the respiratory system. The measurement of serum anti-inflammatory Clara cell protein (CC16) was employed as a peripheral marker of the lung epithelium function. Materials and Methods: A group of 50 casting smelters, 5 locksmiths, 11 sawyers and auxiliary workers exposed to dust containing 14% of aluminum, and a group of 42 controls were examined. Respiratory function tests were performed and forced volume capacity (FVC), forced expiratory volume in 1 s (FEV 1 ), forced expiratory volume in the first percent (FEV 1 %), forced expiratory flows in 50% VC (FEV 50 ), and markers of foundry workers' exposure and body burden, Al concentration in the breathing zone, blood and urine, biomarkers of the effects of exposure, concentration of CC16 and hyaluronic acid (HA) in serum were determined in all examined workers. Additional measurements comprised determinations of serum iron (Fe) levels, myeloperoxidase (MPO), eosinophil cationic protein (ECP), immunoglobulin E (IgE), glutathione S-transferase (GST), and superoxide dismutase (SOD) activity in erythrocytes. Results: The group of casting smelters was characterized by the highest levels of aluminum in urine (Al-U) (43.7 mg L -1 ), high levels of MPO, ECP and IgE, high SOD activity, low CC16 levels, and low activity of GST. Lower Al-U excretion was observed in locksmiths (35.2 mg L -1 ) and sawyers (21.7 mg L -1 ). Serum CC16 proved to be the most sensitive biomarker, showing high inverse relationship with serum Al (Al-S) concentrations in casting smelters (p = 0.006). Conclusions: The study showed that in conditions of occupational exposure, dusts containing Al 2 O 3 < 1 mg m -3 cause changes in the respiratory system and biomarkers in serum, especially in CC16, connected with altered functioning of this system. Changes in concentrations of the examined biomarkers and also in respiratory parameters of the study subjects were observed when Al-U concentration was > 40 mg L -1 .

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Section: Ijomeh 2006;19(4)supporting

confidence: 64%

Serum Clara Cell Protein as an Indicator of Pulmonary Impairment in Occupational Exposure at Aluminum Foundry

Hałatek¹,

Trzcinka-Ochocka²,

Matczak³

et al. 2006

International Journal of Occupational Medicine and Environmental Health

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“…Serum ECP concentrations are elevated in patients with atopic dermatitis, as suggested by many studies. 16,18,21 Therefore, atopic dermatitis had an independent, significant association with elevated ECP in the present study. In contrast, the association between allergic rhinitis and serum ECP does not seem to be clear.…”

Section: Discussionmentioning

confidence: 79%

“…In a Swedish study, the median serum ECP concentration was 28 g/L in children with acute asthma and even higher (32 g/L) in children with mild asthma; in both studies the subjects were not on anti-inflammatory treatment. 18 In our study, the median ECP was 27.5 g/L when the patients had asthma and did not use anti-inflammatory medication, and 13.6 g/L when the patients had symptoms suggestive of asthma (OSLA). Both differed significantly from the value of 11.2 g/L in the healthy controls.…”

Section: Discussionmentioning

confidence: 96%

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Serum eosinophil cationic protein (ECP) and eosinophil protein X (EPX) in childhood asthma: The influence of atopy

et al. 1998

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The purpose of this study was to determine the value of serum measurements of eosinophil cationic protein (ECP) and eosinophil protein X (EPX) in diagnosing asthma in children, and to investigate the influence of concomitant allergic diseases and atopic sensitization, assessed by skin prick tests (SPT), on these markers. ECP and EPX were determined in 36 children with asthma, in 33 children with other symptoms from lower airways disease (OSLA), and in 166 control children. Sixteen children with asthma but no anti‐inflammatory therapy had significantly higher concentrations of ECP and EPX (ECP: 27.5 μg/L, P < 0.001; EPX: 59.9 μg/L, P < 0.001) than the control children (ECP: 11.2 μg/L; EPX: 26.2 μg/L). In the 20 children on anti‐inflammatory therapy, ECP values were similar to those of controls. The children with OSLA (ECP: 13.6 μg/L, P < 0.01; EPX: 47.2 μg/L, P < 0.001) differed significantly from controls. When using the value of 24.7 μg/L (97.5 percentile in the 68 non‐atopic controls) as a pathologic upper limit for ECP, 10 (63%) of the 16 asthmatic children on no maintenance medication, two (10%) of the 20 asthmatics on maintenance therapy, and 11 (33%) of the 33 children with OSLA had high ECP; the same figure was only 18 (11%) in the 166 control children. Both ECP and EPX had a significant association with allergic disorders and with SPT reactivity. In multivariate logistic regression analysis, an elevated ECP was significantly associated with asthma (OR 2.3, 95%Cl 1.1–4.9) and atopic dermatitis (2.9, 1.2–6.9), and an elevated EPX was significantly associated with asthma (2.61, 1.19–5.74) and allergic rhinoconjunctivitis (5.23, 1.46–18.73). We conclude that serum concentrations of both ECP and EPX are higher in asthmatic than in healthy children. However, other allergic diseases, such as allergic rhinoconjunctivitis, atopic dermatitis, and allergic skin sensitization also raise the concentrations of these markers. This limits their usefulness in the diagnosis of childhood asthma. Pediatr. Pulmonol. 1998; 25:167–174. © 1998 Wiley‐Liss, Inc.

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“…Other studies [25, 26]have shown that the levels of ECP in bronchoalveolar lavage and serum correlate. Sensitized subjects tend to have higher serum ECP levels compared to nonsensitized subjects, and asthmatic subjects tend to have higer serum ECP levels compared with nonasthmatic subjects [6, 27]. Koller et al [5]found that ECP measurements may be valuable in identifying those wheezing children with a particularly high risk for development of infantile asthma.…”

Section: Discussionmentioning

confidence: 99%

Increased Eosinophil Cation Protein Level in Sensitized Nonasthmatics Is Linked to Subsequent Hyperresponsiveness to Methacholine

Rasmussen

Lambrechtsen

Siersted

et al. 2000

Int Arch Allergy Immunol

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Background: Increased levels of eosinophil cation protein (ECP) in sensitized subjects may reflect early stages of an ongoing inflammatory process and therefore precede asthma and bronchial hyperreactivity. Aim: To study whether nonasthmatic subjects with sensitization to allergens and increased ECP levels are at a higher risk for subsequent increased bronchial reactivity compared with sensitized nonasthmatics with normal ECP levels. Methods: A prospective study of 240 schoolchildren with a mean age of 13.9 years (range: 12.6–15.9) who were followed up after 6.3 years. Bronchial reactivity was assessed by methacholine provocation testing. Sensitization was defined by one or more positive reactions (>3 mm wheal) to 10 common aeroallergens by skin prick testing. Increased ECP was defined as values above 20 μg/l. This separated the subjects into four categories: group 1: healthy controls without sensitization (n = 147); group 2: sensitized subjects with a serum ECP below 20 μg/l (n = 55); group 3: sensitized subjects with an ECP level at or above 20 μg/l (n = 16), and group 4: all asthmatics (n = 22). Results: Bronchial reactivity was similar in subjects of groups 2 and 3 at baseline (p = 0.8). Six years later, subjects from group 3 were more responsive to methacholine compared with subjects from group 2 (median: 12.7 versus 20.5 μmol; p < 0.05). In a logistic regression with hyperresponsiveness to methacholine at follow-up as dependent variable, the odds ratios (OR) for the groups were, with group 1 as reference: group 2: OR = 2.2 (0.8–6.6: p = 0.2), group 3: 5.9 (1.6–21.7: p < 0.01). Conclusion: Subjects with sensitization and increased ECP levels are subsequently more airway-responsive to methacholine compared with sensitized subjects with normal ECP levels. This supports the hypothesis that sensitization is linked to increased bronchial reactivity through a process in which markers of inflammation are involved.

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Eosinophil cationic protein, myeloperoxidase and tryptase in children with asthma and atopic dermatitis

Cited by 41 publications

References 25 publications

Serum Clara Cell Protein as an Indicator of Pulmonary Impairment in Occupational Exposure at Aluminum Foundry

Serum Clara Cell Protein as an Indicator of Pulmonary Impairment in Occupational Exposure at Aluminum Foundry

Serum eosinophil cationic protein (ECP) and eosinophil protein X (EPX) in childhood asthma: The influence of atopy

Increased Eosinophil Cation Protein Level in Sensitized Nonasthmatics Is Linked to Subsequent Hyperresponsiveness to Methacholine

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