MicroRNA‑195 is associated with regulating the pathophysiologic process of human laryngeal squamous cell carcinoma

Pang, Haifeng; Xu, Xuemei; Dai, Lin‐Lin; Wang, Kun; Yao, Xiaoxi

doi:10.3892/mmr.2018.8523

Cited by 6 publications

(10 citation statements)

References 35 publications

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“…Subsequently, CCK-8, wound healing and Transwell assays, and western blot analysis, were used to examine the effects of miR-195-5p overexpression on the proliferation, migration and invasion of laryngeal cancer cells. Pang et al ( 21 ) reported that miR-195 is associated with the cell viability of the AMC-HN-8 cell line. In the present study, the results of CCK-8 assay revealed that miR-195-5p overexpression significantly inhibited the proliferation of AMC-HN-8 cells compared with the control group ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, miR-195-5p overexpression has been shown to suppress the proliferation of lung cancer cells, and to induce apoptosis and G 0 /G 1 phase arrest ( 30 ). Importantly, miR-195 is reported to be associated with regulating the pathophysiologic process of human laryngeal squamous cell carcinoma ( 21 ). In the present study, the expression of miR-195-5p in laryngeal cancer cells was found to be significantly lower compared with that in normal cells, which was consistent with the expression of miR-195-5p in other types of tumours.…”

Section: Discussionmentioning

confidence: 99%

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MicroRNA‑195‑5p suppresses the proliferation, migration, invasion and epithelial‑mesenchymal transition of laryngeal cancer cells in vitro by targeting E2F3

et al. 2021

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Increasing evidence has indicated that microRNAs (miRNAs/miRs) play an important role in the occurrence and development of various types of cancer. The aim of the present study was to investigate the role and underlying molecular mechanisms of miR-195-5p in laryngeal cancer cell proliferation, migration and invasion. Reverse transcription-quantitative PCR (RT-qPCR) was performed to measure the expression levels of miR-195-5p in laryngeal carcinoma cell lines. The expression levels of miR-195-5p and E2F transcription factor 3 (E2F3) were modified by transfection with miR-195-5p mimics and pcDNA3.1-E2F3. A luciferase reporter assay was used to verify the association between miR-195a-5p and E2F3. Cell Counting Kit-8, cell wound healing and Transwell invasion assays were used to detect the biological functions of laryngeal cancer cells. The expression of epithelial-mesenchymal transition (EMT)-associated genes was evaluated by western blotting and RT-qPCR. The results revealed that the expression of miR-195-5p was decreased in laryngeal cancer cell lines. The overexpression of miR-195-5p inhibited the proliferation, migration, invasion and EMT of laryngeal cancer cells. Dual-luciferase reporter assays revealed that miR-195-5p could directly target E2F3 and that there was a negative association between them. E2F3 overexpression significantly attenuated the inhibitory effects of the overexpression of miR-195-5p on the proliferation, migration, invasion and EMT of laryngeal cancer cells. Collectively, the findings of the present study demonstrated that the overexpression of miR-195-5p significantly inhibited the progression of laryngeal cancer cells, and these effects may be mediated via the downregulation of the expression of E2F3.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

MicroRNA‑195‑5p suppresses the proliferation, migration, invasion and epithelial‑mesenchymal transition of laryngeal cancer cells in vitro by targeting E2F3

et al. 2021

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“…Western blotting was performed to detect the protein expression levels of CDC42 and CCND1 from tissues and cells, as previously described ( 13 ). Total proteins were extracted with RIPA buffer (Beyotime Institute of Biotechnology).…”

Section: Methodsmentioning

confidence: 99%

“…For example, miR-195 has been observed to be downregulated in EOC, and was revealed to decrease cell proliferation and induce apoptosis of human OVCAR-3 cells by inhibiting VEGFR2 and AKT signaling ( 12 ). In addition, miR-195 is downregulated in human laryngeal squamous cell carcinoma, while the overexpression of miR-195 inhibits cell viability, migration and invasion, as well as promotes apoptosis of AMC-HN-8 cells ( 13 ). However, the roles of miR-195 in the cell cycle, migration and invasion of EOC cells are yet to be fully elucidated.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‑195 suppresses cell proliferation, migration and invasion in epithelial ovarian carcinoma via inhibition of the CDC42/CCND1 pathway

et al. 2020

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Epithelial ovarian carcinoma (EOc) is the most common cause of gynecological cancer mortality, and poses a threat to women. MicroRNA-195 (miR-195) has been reported to induce apoptosis of human OVcAR-3 cells by inhibiting the VEGFR2/AKT pathway. However, the role of miR-195 in EOc remains unknown. A previous study reported that cell division cycle 42 (cdc42) can serve as a target gene of miR-195 and mediate malignant progression of esophageal squamous cell carcinoma (EScc). The aim of the present study was to investigate the role of miR-195 in EOc and the regulation in cdc42/ccNd1 pathway. Tissues samples and clinical materials were collected from 78 enrolled patients with EOC to analyze the expression and clinical significance of miR-195, cdc42 and cyclin d1 (ccNd1). Human EOc cell lines OVcA420, OVcAR-3, A2780 and SKOV3 cell lines were used to assess the expression and function of miR-195, cdc42 and ccNd1 in vitro. cell proliferation, the cell cycle and apoptosis, as well as the cell migratory and invasive abilities were detected in vitro using BrdU incorporation, colony formation, wound healing and Transwell invasion assays, along with flow cytometry. miR-195 was downregulated, while CDC42 and ccNd1 were upregulated in human EOc tissues and cells, and the aberrant expression of both was associated with increased EOc malignancy. Moreover, miR-195 expression was negatively correlated with cdc42 and ccNd1 expression levels, and negatively regulated these expression levels. Thus, it was suggested that miR-195 functions as a tumor suppressor, but cdc42 and ccNd1 act as tumor promoters based their abilities to enhance cell proliferation, cell cycle entry, migration and invasion, as well as decrease apoptosis in OVcAR-3 cells. the present results demonstrated that miR-195 inhibited human EOc progression by downregulating cdc42 and CCND1 expression. Furthermore, it was identified that miR-195, cdc42 and ccNd1 may be effective biomarkers for EOc diagnosis and treatment.

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“…According to reports, miRNAs are significant in gene expression, regulation, and cell signaling transduction [8]. MiRNAs engage in multiple steps which are critical for cancer progression, including cell survival, proliferation, specialization, migration, and apoptosis [9][10][11]. Further, miRNAs have been linked with the tumorigenesis of OSCC.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-487a-3p inhibits the growth and invasiveness of oral squamous cell carcinoma by targeting PPM1A

Wang

Zhou

2021

Bioengineered

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Oral squamous cell carcinoma (OSCC) forms the majority of the entire cancerous tumors which occur in the mouth. Current treatment advances, such as surgical resection, chemotherapy, and radiotherapy, have significantly helped reduce OSCC. However, the overall patient survival rate remains relatively low. MiRNAs, a non-coding RNA group, are essential for multiple biological functions, which are essential for the progression of cancer, including survival of the cell, migration, multiplication, differentiation, and apoptosis. The study aimed to explore the existing association between miR-487a-3p and PPM1A and elucidating their role in modulation of proliferation in OSCC cell lines. In this study, we used CAL-27 and TCA-8113 OSCC cell lines and human samples to validate our results. The manifestation of miR-487a-3p and PPM1A was checked using quantitative real-time PCR. The miR-487a-3p and PPM1A binding was investigated through western blot assay and dual-luciferase reporter gene. Functional experiments, including colony formation, CCK-8, and transwell experimentations, were undertaken to validate cells' growth and invasion activities. According to the results, the expression of miR-487a-3p is regulated in the OSCC cell lines compared to normal cells. Moreover, the mimicking of miR-487a-3p significantly reduces the OSCC cell growth and invasion, and PPM1A overexpression exerts oncogenic effects and hinders the anti-oncogenic effects of miR-487a-3p. In conclusion, the study demonstrated that miR-487a-3p might act as a tumor suppressor by inhibiting the growth and invasion of OSCC via regulating PPM1A expression.

show abstract

MicroRNA‑195 is associated with regulating the pathophysiologic process of human laryngeal squamous cell carcinoma

Cited by 6 publications

References 35 publications

MicroRNA‑195‑5p suppresses the proliferation, migration, invasion and epithelial‑mesenchymal transition of laryngeal cancer cells in vitro by targeting E2F3

MicroRNA‑195‑5p suppresses the proliferation, migration, invasion and epithelial‑mesenchymal transition of laryngeal cancer cells in vitro by targeting E2F3

MicroRNA‑195 suppresses cell proliferation, migration and invasion in epithelial ovarian carcinoma via inhibition of the CDC42/CCND1 pathway

MicroRNA-487a-3p inhibits the growth and invasiveness of oral squamous cell carcinoma by targeting PPM1A

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