MicroRNA‑195‑5p suppresses the proliferation, migration, invasion and epithelial‑mesenchymal transition of laryngeal cancer cells in vitro by targeting E2F3

Abstract

Increasing evidence has indicated that microRNAs (miRNAs/miRs) play an important role in the occurrence and development of various types of cancer. The aim of the present study was to investigate the role and underlying molecular mechanisms of miR-195-5p in laryngeal cancer cell proliferation, migration and invasion. Reverse transcription-quantitative PCR (RT-qPCR) was performed to measure the expression levels of miR-195-5p in laryngeal carcinoma cell lines. The expression levels of miR-195-5p and E2F transcription factor 3 (E2F3) were modified by transfection with miR-195-5p mimics and pcDNA3.1-E2F3. A luciferase reporter assay was used to verify the association between miR-195a-5p and E2F3. Cell Counting Kit-8, cell wound healing and Transwell invasion assays were used to detect the biological functions of laryngeal cancer cells. The expression of epithelial-mesenchymal transition (EMT)-associated genes was evaluated by western blotting and RT-qPCR. The results revealed that the expression of miR-195-5p was decreased in laryngeal cancer cell lines. The overexpression of miR-195-5p inhibited the proliferation, migration, invasion and EMT of laryngeal cancer cells. Dual-luciferase reporter assays revealed that miR-195-5p could directly target E2F3 and that there was a negative association between them. E2F3 overexpression significantly attenuated the inhibitory effects of the overexpression of miR-195-5p on the proliferation, migration, invasion and EMT of laryngeal cancer cells. Collectively, the findings of the present study demonstrated that the overexpression of miR-195-5p significantly inhibited the progression of laryngeal cancer cells, and these effects may be mediated via the downregulation of the expression of E2F3.

Circular RNA Pvt1 oncogene (CircPVT1) promotes the progression of papillary thyroid carcinoma by activating the Wnt/β-catenin signaling pathway and modulating the ratio of microRNA-195 (miR-195) to vascular endothelial growth factor A (VEGFA) expression

“…CircPVT1 has been proved to locate mainly in cytoplasm, which meant that it might act as microRNA sponges to regulate downstream gene expression. MiR-195 is dysregulated in several tumors, such as colon cancer [ 56 ], non-small lung cancer [ 57 ], laryngeal cancer [ 58 , 59 ], gastric cancer [ 60 ], cervical cancer [ 61 ], colorectal cancer [ 62 ], breast cancer [ 63 ], lung adenocarcinoma [ 64 ] and osteosarcoma [ 65 ]. Moreover, miR-195 has been demonstrated that play a significant role in PTC [ 66–69 ].…”

Epithelial-to-Mesenchymal Transition in Metastasis: Focus on Laryngeal Carcinoma

“…In most cases, miRNAs act as suppressors of proliferation and lymph-node metastasis and inducers of apoptosis, as in the cases of miR-141, miR-136-5p, miR-204-5p, miR-143-3p, miR-145-5p, and miR-144-3p [ 130 , 131 , 132 , 133 , 134 , 135 ]. Like miR-98, miR-625, and miR-195-5p, other microRNAs can reverse EMT [ 136 , 137 , 138 , 139 ]. Downregulation of miR-203 leads the laryngeal carcinoma cells to acquire stem-cell traits, such as CD44+ [ 139 ].…”

“…Knocking down Snail has been shown to enhance chemosensitivity [ 194 ]. In laryngeal carcinoma cell lines, successful attempts to reverse EMT have been made with the introduction of miRNAs [ 136 , 195 , 196 , 197 , 198 ], inhibitors of long-noncoding RNA [ 199 , 200 ], and flavonoids such as genistein, dihydroartemisinin, and brusatol [ 201 , 202 , 203 ]. Moreover, a higher expression of estrogen receptor-β has been found to correlate with lower EMT traits in laryngeal carcinoma.…”

Circ_001422 aggravates osteosarcoma progression through targeting miR-497-5p/E2F3 axis

“…E2F3, which belongs to the E2F TF family, participates in cancer genesis and progression, and its expression is increased within diverse cancers like bladder cancer (BLCA), laryngeal cancer, osteosarcoma and breast cancer (BRCA) [26][27][28][29] . In addition, various miRNAs like miR-22, miR-194-5p or miR-195-5p, can regulate cancer genesis and progression via E2F3, suggesting that E2F3 may be the possible anticancer therapeutic target [30,26,27] . TargetScan online prediction identi ed E2F3 as miR-497-5p's potential target.…”