MicroRNA-487a-3p inhibits the growth and invasiveness of oral squamous cell carcinoma by targeting PPM1A

Abstract

Oral squamous cell carcinoma (OSCC) forms the majority of the entire cancerous tumors which occur in the mouth. Current treatment advances, such as surgical resection, chemotherapy, and radiotherapy, have significantly helped reduce OSCC. However, the overall patient survival rate remains relatively low. MiRNAs, a non-coding RNA group, are essential for multiple biological functions, which are essential for the progression of cancer, including survival of the cell, migration, multiplication, differentiation, and apoptosis. The study aimed to explore the existing association between miR-487a-3p and PPM1A and elucidating their role in modulation of proliferation in OSCC cell lines. In this study, we used CAL-27 and TCA-8113 OSCC cell lines and human samples to validate our results. The manifestation of miR-487a-3p and PPM1A was checked using quantitative real-time PCR. The miR-487a-3p and PPM1A binding was investigated through western blot assay and dual-luciferase reporter gene. Functional experiments, including colony formation, CCK-8, and transwell experimentations, were undertaken to validate cells' growth and invasion activities. According to the results, the expression of miR-487a-3p is regulated in the OSCC cell lines compared to normal cells. Moreover, the mimicking of miR-487a-3p significantly reduces the OSCC cell growth and invasion, and PPM1A overexpression exerts oncogenic effects and hinders the anti-oncogenic effects of miR-487a-3p. In conclusion, the study demonstrated that miR-487a-3p might act as a tumor suppressor by inhibiting the growth and invasion of OSCC via regulating PPM1A expression.

lncRNA TSPEAR-AS2, a Novel Prognostic Biomarker, Promotes Oral Squamous Cell Carcinoma Progression by Upregulating PPM1A via Sponging miR-487a-3p

“…It can control TGF-beta/Smad19-21 and mitogen-activated protein kinase22 cellular signaling channels, and proliferating, invading, and 5 Disease Markers migrating processes of cells [42,43]. In OSCC, PPM1A has been reported to be highly expressed and promoted the proliferation and metastasis of OSCC cells, whereas how PPM1A controls the mentioned activities requires in-depth studies [40]. In this study, we found PPM1A may be a target of miR-487a-3p.…”

“…Previously, miR-487a-3p has been reported to be overexpressed in several tumors, such as colon cancer and gastric cancer [38,39]. Importantly, in OSCC, miR-487a-3p was found to display an upregulated level and suppress the proliferation and metastasis of OSCC cells [40]. These findings suggested TSPEAR-AS2 may display its oncogenic roles via sponging miR-487a-3p.…”

The regulation of long non-coding RNA 00958 (LINC00958) for oral squamous cell carcinoma (OSCC) cells death through absent in melanoma 2 (AIM2) depending on microRNA-4306 and Sirtuin1 (SIRT1) in vitro

“…LncRNAs are noncoding RNAs with length longer than 200 nucleotides [3]. The sponge effect of LncRNA on microRNA (miRNA) which participates in OSCC survival [4][5][6], is one of the important mechanisms of the two interactions. Some studies have demonstrated that lncRNA can regulate miRNA aggregation and biological function as competitive endogenous RNA (ceRNA) [7].…”

Long non-coding RNA CASC9 promotes tumor progression in oral squamous cell carcinoma by regulating microRNA-545-3p/laminin subunit gamma 2

“…miRNAs, 22 nucleotides in length, are a large group of small non-coding RNAs that can exert crucial biological functions by binding to the three prime untranslated region (3 UTR) of their target genes [16,17]. Recently, many miRNAs have been shown to participate in OSCC progression by targeting the genes, including miR-579 [18], miR-487a-3p [19], and miR-210-3p [15]. miR-545-3p, a member of the miRNA family, exerts an inhibitory function in multiple cancers [20][21][22].…”