Biopolymer-based membranes associated with osteogenic growth peptide for guided bone regeneration

Saska, Sybele; Pigossi, Suzane Cristina; Oliveira, Guilherme José Pimentel Lopes de; Teixeira, Lucas Novaes; Capela, Marisa Veiga; Gonçalves, Andreia M.; Oliveira, Paulo Tambasco de; Messaddeq, Younès; Ribeiro, Sidney J. L.; Gaspar, Ana Maria Minarelli; Marchetto, Reinaldo

doi:10.1088/1748-605x/aaaa2d

Cited by 17 publications

(9 citation statements)

References 43 publications

(290 reference statements)

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“…Guided bone regeneration membrane tubes can be used as a support material in bone tissue engineering, the combination of growth factors and membrane tubes can enhance proliferation, chemotaxis, and differentiation of osteogenic cells. 23,24 With good biocompatibility and mechanical properties, the novel n-CDHA-MAC membrane tube has good potential to be used in the bone tissue engineering.…”

Section: Discussionmentioning

confidence: 99%

Experimental study on repair of large segmental bone defects of goat femur by nano calcium-deficient hydroxyapatite-multi (amino acid) copolymer membrane tubes

et al. 2021

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Objective The purpose of this study was to observe feasibility of nano calcium-deficient hydroxyapatite-multi (amino acid) copolymer (n-CDHA-MAC) membrane tubes in repairing goat femurs’ large defects. Methods Twelve goats were divided into two groups, whose femurs were created 30 mm segmental bone defects and then implants were performed. In experimental group, the bone defect of right femur was reconstructed by n-CDHA-MAC membrane tube, while left side was reconstructed by allogenic bone tube in control group. Every three goats were sacrificed at 4, 8, 16, 24 weeks after operation respectively. General observation, X-ray analysis, histology, Scanning electron microscope (SEM) examination and protein level comparison of BMP-2 were conducted to evaluate the effects of repairing segmental bone defects. Results All goats recovered well from anesthesia and surgical interventions. The radiographic evaluations showed that periosteal reaction outside of the membrane tubes and allogenic bone tubes were observed 4 weeks after surgery. At 16 weeks, callus was continuously increased in experimental group, which was more obvious than control group. At 24 weeks, callus outside of the membrane tubes connected together. Histologic evaluation showed fibro-cartilage callus was evolved into bony callus in experimental group, which was more obvious than control group at 8 and 16 weeks. The protein expression level of BMP-2 increased at 4, 8 weeks and peaked at 16 weeks in experimental groups. There were statistical differences at 8 and 16 weeks ( P < 0.05). At each time point in 8, 16, 24 weeks after surgery, the bending stiffness, torsional stiffness and compressive strength of the two groups were similar, and there was no significant difference ( P > 0.05). Conclusions This novel surface degradation n-CDHA-MAC membrane tube has good ability to maintain enough membrane space, which can provide long-term and stable biomechanical support for large bone defects and integrate well with the surrounding bone.

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Section: Discussionmentioning

confidence: 99%

Experimental study on repair of large segmental bone defects of goat femur by nano calcium-deficient hydroxyapatite-multi (amino acid) copolymer membrane tubes

et al. 2021

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“…OGP has been incorporated into regenerative membranes used in the GTRT. For instance, it has been shown that membranes based on biopolymers combined with OGP-(10-14) stimulate the proliferation and activity of osteoblasts in vitro, as well as the repair of bone tissue in vivo (Saska et al, 2018). Both, in vitro and in vivo experiments showed that the material is beneficial for cell adhesion, has good biocompatibility, enhances the expressions of osteogenic-related genes, and accelerates bone regeneration.…”

Section: Osteogenic Growth Peptidementioning

confidence: 99%

Bioactive Synthetic Peptides for Oral Tissues Regeneration

et al. 2021

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Regenerative therapy in oral tissues has gained relevance since tissue loss due to congenital or acquired diseases as well as trauma is a major health problem worldwide. Regeneration depends on the natural capacity of the body and the use of biomaterials and bioactive molecules that can module the processes to replace lost or damaged tissues and restore function. The combined use of scaffolds, cells, and bioactive molecules such as peptides is considered the best approach to achieve tissue regeneration. These peptides can induce diverse cellular processes as they can influence cell behavior and also can modify scaffold properties, giving as a result the enhancement of cell adhesion, proliferation, migration, differentiation, and biomineralization that are required given the complex nature of oral tissues. Specifically, synthetic peptides (SP) have a positive influence on scaffold biocompatibility since in many cases they can mimic the function of a natural peptide or a full-length protein. Besides, they are bioactive molecules easy to produce, process, and modify, and they can be prepared under well-defined and controlled conditions. This review aims to compile the most relevant information regarding advances in SP for dental and periodontal tissue regeneration, their biological effects, and their clinical implications. Even though most of the SP are still under investigation, some of them have been studied in vitro and in vivo with promising results that may lead to preclinical studies. Besides there are SP that have shown their efficacy in clinical trials such as P11-4 for enamel regeneration or caries prevention and ABM/P-15 for cementum, periodontal ligament (PDL), and alveolar bone on a previously calculus- and biofilm-contaminated zone. Also, some SP are commercially available such as PTH1-34 and PepGen P-15 which are used for bone defects treatment.

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“…In view of these requirements, several biomaterials have been highlighted in reconstructive surgery and regenerative medicine due to the ability to mimic tissue microstructure, allowing reactions inherent to the extracellular matrix and in the regulation of cellular phenotypes. Among these biomaterials, polymeric matrices, natural or synthetic, have shown satisfactory preclinical and clinical results [6].…”

Section: Introductionmentioning

confidence: 99%

In Vivo Biological Behavior of Polymer Scaffolds of Natural Origin in the Bone Repair Process

et al. 2021

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Autologous bone grafts, used mainly in extensive bone loss, are considered the gold standard treatment in regenerative medicine, but still have limitations mainly in relation to the amount of bone available, donor area, morbidity and creation of additional surgical area. This fact encourages tissue engineering in relation to the need to develop new biomaterials, from sources other than the individual himself. Therefore, the present study aimed to investigate the effects of an elastin and collagen matrix on the bone repair process in critical size defects in rat calvaria. The animals (Wistar rats, n = 30) were submitted to a surgical procedure to create the bone defect and were divided into three groups: Control Group (CG, n = 10), defects filled with blood clot; E24/37 Group (E24/37, n = 10), defects filled with bovine elastin matrix hydrolyzed for 24 h at 37 °C and C24/25 Group (C24/25, n = 10), defects filled with porcine collagen matrix hydrolyzed for 24 h at 25 °C. Macroscopic and radiographic analyses demonstrated the absence of inflammatory signs and infection. Microtomographical 2D and 3D images showed centripetal bone growth and restricted margins of the bone defect. Histologically, the images confirmed the pattern of bone deposition at the margins of the remaining bone and without complete closure by bone tissue. In the morphometric analysis, the groups E24/37 and C24/25 (13.68 ± 1.44; 53.20 ± 4.47, respectively) showed statistically significant differences in relation to the CG (5.86 ± 2.87). It was concluded that the matrices used as scaffolds are biocompatible and increase the formation of new bone in a critical size defect, with greater formation in the polymer derived from the intestinal serous layer of porcine origin (C24/25).

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Biopolymer-based membranes associated with osteogenic growth peptide for guided bone regeneration

Cited by 17 publications

References 43 publications

Experimental study on repair of large segmental bone defects of goat femur by nano calcium-deficient hydroxyapatite-multi (amino acid) copolymer membrane tubes

Experimental study on repair of large segmental bone defects of goat femur by nano calcium-deficient hydroxyapatite-multi (amino acid) copolymer membrane tubes

Bioactive Synthetic Peptides for Oral Tissues Regeneration

In Vivo Biological Behavior of Polymer Scaffolds of Natural Origin in the Bone Repair Process

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