In vivo-like 3-D model for sodium nitrite- and acrylamide-induced hepatotoxicity tests utilizing HepG2 cells entrapped in micro-hollow fibers

Chu, Qiang; Zhao, Yiying; Shi, Xuer; Han, Wen; Zhang, Yanzhen; Zheng, Xiaodong; Zhu, Jing

doi:10.1038/s41598-017-13147-z

Cited by 18 publications

(16 citation statements)

References 41 publications

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“…All these characteristics combined confirmed the feasibility of using sterilized PVDF material to construct the bioreactor according to its high permeability and capacity for maintaining cell growths (de Bartolo et al, 2006). In this section, we conducted the same type of experiment presented by Chu et al (2017) to assure the integrity and effectiveness of this particular batch of materials.…”

Section: Characterization Of the Hollow Fiber Materialsmentioning

confidence: 99%

“…The absorbance was measured at 570 nm using a spectrophotometer. This method is referred to the one described by Chu et al (2017).…”

Section: Cell Viability Assaysmentioning

confidence: 99%

“…The cells were observed under the phase contrast fluorescent microscope. This method is referred to the one described by Chu et al (2017).…”

Section: Fluorescent Stain Of Nucleimentioning

confidence: 99%

“…In this section, we used very similar methods with the ones depicted by Chu et al (2017). Yet with different types of cells lines (HepG2 cells in Chu et al (2017) and LO2 cells in this study), we have the rationale to establish new conditions for further testing.…”

Section: Construction and Optimization Of The Modelmentioning

confidence: 99%

“…The cell were cultured according to the methods described by Chu et al (2017). Briefly, for the monolayer culture, LO2 cells were cultured in Dulbecoo's modified Eagle medium (DMEM, Gibco) containing 100 U/ml streptomycin, 100 U/ml penicillin, and 10% (v/v) FBS, and were later incubated at 5% (v/v) CO 2 , 37 °C.…”

Section: Cell Culture and Treatmentsmentioning

confidence: 99%

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Toxicity testing of four silver nanoparticle-coated dental castings in 3-D LO2 cell cultures

Zhao¹,

Chu²,

Shi³

et al. 2018

J. Zhejiang Univ. Sci. B

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Abstract:To address the controversial issue of the toxicity of dental alloys and silver nanoparticles in medical applications, an in vivo-like LO2 3-D model was constructed within polyvinylidene fluoride hollow fiber materials to mimic the microenvironment of liver tissue. The use of microscopy methods and the measurement of liver-specific functions optimized the model for best cell performances and also proved the superiority of the 3-D LO2 model when compared with the traditional monolayer model. Toxicity tests were conducted using the newly constructed model, finding that four dental castings coated with silver nanoparticles were toxic to human hepatocytes after cell viability assays. In general, the toxicity of both the castings and the coated silver nanoparticles aggravated as time increased, yet the nanoparticles attenuated the general toxicity by preventing metal ion release, especially at high concentrations.

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Section: Characterization Of the Hollow Fiber Materialsmentioning

confidence: 99%

“…The absorbance was measured at 570 nm using a spectrophotometer. This method is referred to the one described by Chu et al (2017).…”

Section: Cell Viability Assaysmentioning

confidence: 99%

“…The cells were observed under the phase contrast fluorescent microscope. This method is referred to the one described by Chu et al (2017).…”

Section: Fluorescent Stain Of Nucleimentioning

confidence: 99%

Section: Construction and Optimization Of The Modelmentioning

confidence: 99%

Section: Cell Culture and Treatmentsmentioning

confidence: 99%

See 3 more Smart Citations

Toxicity testing of four silver nanoparticle-coated dental castings in 3-D LO2 cell cultures

Zhao¹,

Chu²,

Shi³

et al. 2018

J. Zhejiang Univ. Sci. B

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Progression of conventional hepatic cell culture models to bioengineered HepG2 cells for evaluation of herbal bioactivities

et al. 2018

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Cancer cell lines of human tissue origin have been extensively used to investigate antiproliferative activity and toxicity of herbal extracts, isolated compounds, and anticancer drugs. These cell lines are genetically and/or epigenetically well characterized to determine the altered expression of proteins within given cellular pathways and critical genes in cancer. Human derived hepatoma (HepG2) cell line has been extensively exploited to examine cytoprotective, antioxidative, hepatoprotective, anti-hepatoma, hypocholesterolemic, anti-steatosis, bioenergetic homeostatic and anti-insulin resistant properties. Moreover, mechanism of action of various botanicals and bioactive constituents has been reported using these cells. HepG2 cells have significant differences as compared to primary hepatocytes with respect to expression of cytochrome P450 enzymes and xenobiotic receptors in conventional in vitro culture conditions. Therefore, strategies have been employed to overcome limitations of two dimensional (2D) in vitro HepG2 cell culture in order to recognize functional biomarkers more accurately and to boost its predictive value in clinical research. In consequence, three dimensional (3D) human hepatoma cell culture models are being developed as a resource to achieve these goals of simulating the in vivo tumor microenvironment. It is assumed that bioengineered 3D hepatoma cell culture models can provide significant assistance in scrutinizing the molecular response of herbal natural products to recognize novel prognostic targets and crucial biomarkers in treatment strategies for cancer patients in near future.

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Introducing CELLBLOKS®: a novel organ-on-a-chip platform allowing a plug-and-play approach towards building organotypic models

Llabjani¹,

Siddique²,

Macos³

et al. 2022

In vitro models

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Human organs are structurally and functionally complex systems. Their function is driven by the interactions between many specialised cell types, which is difficult to unravel on a standard Petri dish format. Conventional "Petri dish" approaches to culturing cells are static and self-limiting. However, current organ-on-a-chip technologies are difficult to use, have a limited throughput and lack compatibility with standard workflow conditions. We developed CELLBLOKS ® as a novel "plug-andplay" organ-on-a-chip platform that enables straightforward creation of multiple cell-type organ-specific microenvironments. Herein, we demonstrate its advantages by building a liver model representative of live tissue function. CELLBLOKS ® allows one to systematically test and identify various cell combinations that replicate optimal hepatic relevance. The combined interactions of fibroblasts, endothelial cells and hepatocytes were analysed using hepatic biochemistry (CYP3A4 and urea), cellular proliferation indices and transporter activities (albumin). The results demonstrate that optimal liver function can be achieved by exploiting crosstalk in co-culture combinations compared to conventional mono-culture. The optimised CELLBLOKS ® liver model was tested to analyse drug-induced liver toxicity using tamoxifen. The data suggests that our CELLBLOKS ® liver model is highly sensitive to toxic insult compared to mono-culture liver models. In summary, CELLBLOKS ® provides a novel cell culture technology for creating human-relevant organotypic models that are easy and straightforward to establish in laboratory settings.

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In vivo-like 3-D model for sodium nitrite- and acrylamide-induced hepatotoxicity tests utilizing HepG2 cells entrapped in micro-hollow fibers

Cited by 18 publications

References 41 publications

Toxicity testing of four silver nanoparticle-coated dental castings in 3-D LO2 cell cultures

Toxicity testing of four silver nanoparticle-coated dental castings in 3-D LO2 cell cultures

Progression of conventional hepatic cell culture models to bioengineered HepG2 cells for evaluation of herbal bioactivities

Introducing CELLBLOKS®: a novel organ-on-a-chip platform allowing a plug-and-play approach towards building organotypic models

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