Toxicity testing of four silver nanoparticle-coated dental castings in 3-D LO2 cell cultures

Zhao, Yiying; Chu, Qiang; Shi, Xuer; Zheng, Xiaodong; Shen, Xiaojing; Zhang, Yanzhen

doi:10.1631/jzus.b1600482

Cited by 14 publications

(5 citation statements)

References 28 publications

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“…The following day, cells were treated with different concentrations of CACN for 24 h. At the end of the treatment, the medium was carefully discarded and fresh medium containing 0.5 mg/mL methyl-thiazolyl-tetrazolium (MTT) was subsequently added to each well followed by additional 4 h incubation at 37°C. Finally, the MTT formazan precipitate was dissolved in 150 μ L DMSO and the absorbance was measured at 570 nm using a microplate spectrophotometer [ 26 ].…”

Section: Methodsmentioning

confidence: 99%

Cherry Anthocyanins Regulate NAFLD by Promoting Autophagy Pathway

Chu

Zhang

Chen

et al. 2019

Oxidative Medicine and Cellular Longevity

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Nonalcoholic fatty liver disease (NAFLD) is a common chronic disease that threatens human health, and present therapies remain limited due to the lack of effective drugs. Lipid metabolic disturbance and oxidative stress have strong links to the development of NAFLD, while autophagy was generally accepted as a key regulatory mechanism on these steps. Our previous studies indicated that cherry anthocyanins (CACN) protected against high fat diet-induced obesity and NALFD in C57BL/6 mice, while the underlying molecule mechanism is still unclear. Thus, in this study, we show that CACN protect against oleic acid- (OA-) induced oxidative stress and attenuate lipid droplet accumulation in NAFLD cell models. According to the results of a transmission electron microscope (TEM), western blot, immunofluorescence (IF), and adenovirus transfection (Ad-mCherry-GFP-LC3B), autophagy is in accordance with the lipid-lowering effect induced by CACN. Further studies illustrate that CACN may activate autophagy via mTOR pathways. In addition, an autophagy inhibitor, 3-methyladenine (3-MA), was applied and the result suggested that autophagy indeed participates in the lipid clearance process in OA-induced lipid accumulation. All these results indicate that the positive effects of CACN on OA-induced hepatic lipid accumulation are mediated via activating autophagy, showing a potential target for the therapeutic strategy of NAFLD.

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Section: Methodsmentioning

confidence: 99%

Cherry Anthocyanins Regulate NAFLD by Promoting Autophagy Pathway

Chu

Zhang

Chen

et al. 2019

Oxidative Medicine and Cellular Longevity

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“…Long-term metal nanoparticles may cause cellular or systemic toxicity. These effects have been studied in different studies in laboratory and animal conditions in the short term (Akay et al, 2018;Chan et al, 2015;Garcia-Contreras et al, 2014;Hashimoto et al, 2016;Heravi et al, 2013;Laiteerapong et al, 2019;Tabari et al, 2017;Takamiya et al, 2016;Zand et al, 2016;Zhao et al, 2018) (Table 1).…”

Section: Discussionmentioning

confidence: 99%

“…After evaluating the full text of the remaining 29 articles, 18 more studies were deleted. Finally, the full text of the 10 main studies was included in this review (Akay, Cevik, Karakis, & Sevim, 2018;Chan, Zhang, & Cheung, 2015;Garcia-Contreras et al, 2014;Hashimoto et al, 2016;Heravi et al, 2013;Laiteerapong, Reichl, Hickel, & Högg, 2019;Tabari, Hosseinpour, Parashos, Khozestani, & Rahimi, 2017;Takamiya et al, 2016;Zand et al, 2016;Zhao et al, 2018).…”

Section: Data Extractionmentioning

confidence: 99%

“…In the present systematic study, 10 articles were reviewed. Eight in vitro studies and two in animal models evaluated the toxic effects of metal nanoparticles used in dentistry (Akay et al, 2018;Chan et al, 2015;Garcia-Contreras et al, 2014;Hashimoto et al, 2016;Heravi et al, 2013;Laiteerapong et al, 2019;Tabari et al, 2017;Takamiya et al, 2016;Zand et al, 2016;Zhao et al, 2018).…”

Section: Qualitative Analysismentioning

confidence: 99%

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systematic review on the applications of nanoparticles in dentistry

Barjoee¹,

Fathi

2022

ijhs

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Background: Nanotechnology plays a very important role in producing dental compounds. In recent years, a variety of nanoparticles have been used to enhance the properties of these compounds. However, less attention has been paid to the implications of the toxic effects of metallic nanoparticles. The purpose of this systematic review was to review studies evaluating the toxic effects of metal nanoparticles used in dentistry to determine whether long-term use of these compounds has toxic effects. Methods: Electronic search of studies in Pubmed, Scopus, Medline, and google scholar databases was performed until December 2020, according to PRISMA guidelines (preferential reporting items for systematic review and meta-analysis). Results: During the initial research, 325 papers were collected. After reviewing the texts, eliminating duplicates, and applying inclusion criteria, only 10 of these were selected for qualitative review. The results of the studies showed that the use of short-term metallic nanoparticles does not result in increased cytotoxicity. Conclusion: Dental compounds containing metallic nanoparticles had higher cytotoxic activity than unmodified raw materials, but overall, during the short-term review, these studies did not indicate a significant difference in cytotoxicity.

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“…A novel 3D culture in LOD2 cells which resemble an in vivo-like environment assessed the toxicity of dental castings coated with silver nanoparticle. Cell viability tests revealed the materials have a time-dependent toxicity(Zhao et al 2018).Moreover, other metal-based alloys such as Cu-based alloy (Thermobond), Ni-Cr alloys (Remanium CS, Heranium NA, Wiron 99, CB Soft) and Co-Cr alloy (Wirobond C) used commercially have been stated to cause toxicity in vitro in which Cu ions have more detrimental effects in cell viability. However, Bioherador N alloy had meaningfully less cytotoxicity than the other ones(Al-Hiyasat et al 2003).…”

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confidence: 99%

A review on potential toxicity of dental material and screening their biocompatibility

Shahi

Özcan

Dizaj

et al. 2019

Toxicology Mechanisms and Methods

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Objectives: A wide range of compounds are utilized in dentistry such as dental composites, resins and implants. The successful clinical use of dental materials rely on their physiochemical properties as well as biological and toxicological reliability. Different local and systemic toxicities of dental materials have been reported. Placement of these materials in oral cavity for a long time period might yield in unwanted reactions. An extensive variety of materials is used in dentistry including filling materials, restorative materials, intracanal medicines, prosthetic materials, different types of implants, liners and irrigants. The increasing rate in development of the novel materials with applications in the dental field has led to an increased consciousness of the biological risks and tempting restrictions of these materials. The biocompatibility of a biomaterial used for the replacement or filling of biological tissue like teeth always had a high concern within the health care disciplines for patients. Materials and Methods: Any material used in humans should be tested before clinical application. There are many tests evaluating biocompatibility of these materials at the point of in vitro, in vivo and clinical investigations. Results: The current review discusses the potential toxicity of dental material and screening of their biocompatibility. Clinical Relevance: It is essential to use healthy and safe materials medical approaches. In dentistry, application of different material in long-term oral usage demands low or non-toxic agents gains importance for both patients and the staff. Furthermore, screening tests should evaluate any potential toxicity before clinical application.

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Toxicity testing of four silver nanoparticle-coated dental castings in 3-D LO2 cell cultures

Cited by 14 publications

References 28 publications

Cherry Anthocyanins Regulate NAFLD by Promoting Autophagy Pathway

Cherry Anthocyanins Regulate NAFLD by Promoting Autophagy Pathway

systematic review on the applications of nanoparticles in dentistry

A review on potential toxicity of dental material and screening their biocompatibility

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