WRKY70 prevents axenic activation of plant immunity by direct repression of <i>SARD1</i>

Zhou, Man; Lu, You; Bethke, Gerit; Harrison, Brian T.; Hatsugai, Noriyuki; Katagiri, Fumiaki; Glazebrook, Jane

doi:10.1111/nph.14846

Cited by 69 publications

(69 citation statements)

References 50 publications

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“…In turn, the expression of SARD1 and CBP60g is regulated by PCRK1 (AT3G09830) and PCRK2 who also interact with FLS2 (Kong et al 2016). SARD1 expression is also regulated by WRKY70 (Zhou et al 2018). Here, we found that IOS1, SARD1 and WRKY70 were expressed higher in Col-0 than in pad4-1 sid2-1 (Additional file 6: Table S5 and Table S6).…”

Section: Discussionmentioning

confidence: 67%

Transcriptome analysis reveals pathways facilitating the growth of tobacco powdery mildew in Arabidopsis

Zhang

Yang

et al. 2019

Phytopathol Res

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Powdery mildew (PM) fungi are biotrophic pathogens that rely on living hosts to survive and thrive. However, their colonization is restricted by host defenses at both the penetration and post-penetration stages. The tobacco PM strain Golovinomyces cichoracearum (Gc) SICAU1 has overcome penetration resistance of Arabidopsis but its growth is arrested by post-penetration resistance. While Gc SICAU1 only poorly grows in Arabidopsis Col-0 wild-type plants, it can sustainably grow for more than 20 days on the same infected leaves of the double mutant pad4-1 sid2-1 that is defective in both the synthesis and signaling of salicylic acid (SA). To understand the underlying molecular mechanisms, we conducted a comparative transcriptome analysis between Col-0 and pad4-1 sid2-1 in response to Gc SICAU1. We found that 4811 genes were differentially expressed more than four-fold between any two of the measured seven time points (0, 1, 3, 6, 8, 10 and 12 days post-inoculation). Gene expression pattern analysis suggests that differential expression of 348 genes and 190 genes may explain resistance in Col-0 and susceptibility in pad4-1 sid2-1, respectively. Gene Ontology (GO) analysis suggests that Gc SICAU1 might be arrested in Col-0 by both pattern-triggered immunity and SA-dependent defense. By contrast, its sustained growth in pad4-1 sid2-1 may be attributable to the activation of a detoxification pathway that is normally repressed by the SA-signaling pathway. Taken together, our results suggest that multiple distinct, yet interconnected pathways control the growth of tobacco powdery mildew in Arabidopsis.

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Section: Discussionmentioning

confidence: 67%

Transcriptome analysis reveals pathways facilitating the growth of tobacco powdery mildew in Arabidopsis

Zhang

Yang

et al. 2019

Phytopathol Res

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“…TaWRKY44 may act as a positive regulator in drought, salt and osmotic stress responses (Xiatian et al, ). The WRKY transcription factors, which specifically bind to cis‐acting elements, can regulate the expression of the corresponding genes, and the W‐box is mainly present in metabolism‐ and environmental stress‐related gene promoter regions (Gong et al, ; Zhou et al, ). TaWRKY33 might be involved in the interaction of the ABA signalling pathway and might be associated with abiotic stress tolerance in plants (He et al, ).…”

Section: Discussionmentioning

confidence: 99%

Retracted: Cytosolic glyceraldehyde‐3‐phosphate dehydrogenase 2/5/6 increase drought tolerance via stomatal movement and reactive oxygen species scavenging in wheat

Zhang

Lei

Deng

et al. 2020

Plant Cell & Environment

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Drought is a major threat to wheat growth and crop productivity. However, there has been only limited success in developing drought‐hardy cultivars. This lack of progress is due, at least in part, to a lack of understanding of the molecular mechanisms of drought tolerance in wheat. Here, we evaluated the potential role of three cytosolic glyceraldehyde‐3‐phosphate dehydrogenases (TaGAPC2/5/6) under drought stress in wheat and Arabidopsis. We found that TaGAPC2/5/6 all positively responded to drought stress via reactive oxygen species (ROS) scavenging and stomatal movement. The results of yeast co‐transformation and electrophoretic mobility shift assay showed that TaWRKY33 acted as a direct regulator of TaGAPC2/5/6 genes. The dual luciferase reporter assay indicated that TaWRKY33 positively activated the expression of TaGAPC2/5/6. The results of bimolecular fluorescence complementation and yeast two‐hybrid system demonstrated that TaGAPC2/5/6 interacted with phospholipase Dδ (PLDδ). We then demonstrated that TaGAPC2/5/6 positively promoted the activity of TaPLDδ in vitro and in vivo. Furthermore, lower PLDδ activity in RNAi wheat could lead to less PA accumulation, causing higher stomatal aperture sizes under drought stress. In summary, our results establish a new positive regulatory mechanism of TaGAPCs which helps wheat fine‐tune their drought responses.

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“…The expression of ALD1 and SARD4 is positively regulated by the transcription factors SARD1 and CALMODULIN BINDING PROTEIN 60g (CBP60g) (Sun et al, 2015), which also regulate expression of SALICYLIC ACID INDUCTION DEFICIENT2 (SID2), encoding an SA biosynthesis enzyme that is required for SA production upon pathogen infection in Arabidopsis (Wildermuth et al, 2001;Zhang et al, 2010;Wang et al, 2011). It was recently found that expression of SARD1 and CBP60g is regulated by the transcription factors TGA1, TGA4, and WRKY70 (Sun et al, 2018;Zhou et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

A MPK3/6-WRKY33-ALD1-Pipecolic Acid Regulatory Loop Contributes to Systemic Acquired Resistance

et al. 2018

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Plants induce systemic acquired resistance (SAR) upon localized exposure to pathogens. Pipecolic acid (Pip) production via AGD2-LIKE DEFENSE RESPONSE PROTEIN1 (ALD1) is key for SAR establishment. Here, we report a positive feedback loop important for SAR induction in We showed that local activation of the MAP kinases MPK3 and MPK6 is sufficient to trigger Pip production and mount SAR. Consistent with this, mutations in or led to compromised Pip accumulation upon inoculation with the bacterial pathogen pv DC3000 () AvrRpt2, which triggers strong sustained MAPK activation. By contrast, pv and , which induce transient MAPK activation, trigger Pip biosynthesis and SAR independently of MPK3/6. expression, Pip accumulation, and SAR were compromised in mutants defective in the MPK3/6-regulated transcription factor WRKY33. Chromatin immunoprecipitation showed that WRKY33 binds to the promoter. We found that Pip triggers activation of MPK3 and MPK6 and that MAPK activation after AvrRpt2 inoculation is compromised in and mutants. Collectively, our results reveal a positive regulatory loop consisting of MPK3/MPK6, WRKY33, ALD1, and Pip in SAR induction and suggest the existence of distinct SAR activation pathways that converge at the level of Pip biosynthesis.

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WRKY70 prevents axenic activation of plant immunity by direct repression of SARD1

Cited by 69 publications

References 50 publications

Transcriptome analysis reveals pathways facilitating the growth of tobacco powdery mildew in Arabidopsis

Transcriptome analysis reveals pathways facilitating the growth of tobacco powdery mildew in Arabidopsis

Retracted: Cytosolic glyceraldehyde‐3‐phosphate dehydrogenase 2/5/6 increase drought tolerance via stomatal movement and reactive oxygen species scavenging in wheat

A MPK3/6-WRKY33-ALD1-Pipecolic Acid Regulatory Loop Contributes to Systemic Acquired Resistance

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