Transcriptome analysis reveals pathways facilitating the growth of tobacco powdery mildew in Arabidopsis

Li, Ran; Zhang, Lingli; Yang, Xue‐Mei; Cao, Xiaolong; Wang, Yingge; Ma, Xiao; Chandran, Viswanathan; Fan, Jing; Yang, Hui; Shang, Jing; Jun, Zhao; Xiao, Shaobo; Li, Yan; Wang, Wenming

doi:10.1186/s42483-019-0012-z

Cited by 6 publications

(6 citation statements)

References 66 publications

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“…In our experiment, this is also an intriguing biological phenomenon that highlights the complexity of obligate biotrophic plant-pathogen interactions who are direct target of the CmbHLH87 protein and how to interact in response to pathogen resistance need to be further research. PM symptoms herein were observed in WT tobacco seedlings at 7 dpi of G. cichoracearum, and the phenotypes were not more robust than those of previous reports: PM disease occurs abundantly in tobacco plants at 12 dpi of the PM strain G. cichoracearum SICAU1 (Li et al, 2019). Different tobacco accessions may show various susceptible PM disease phenotypes to different PM isolates.…”

Section: Discussioncontrasting

confidence: 75%

Expression of Pumpkin CmbHLH87 Gene Improves Powdery Mildew Resistance in Tobacco

et al. 2020

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Powdery mildew (PM), caused by Podosphaera xanthii, is a major threat to the global cucurbit yield. The molecular mechanisms underlying the PM resistance of pumpkin (Cucurbita moschata Duch.) are largely unknown. A homolog of the basic helix-loop-helix (bHLH) transcription factor was previously identified through a transcriptomic analysis of a PM-resistant pumpkin. In this study, this bHLH homolog in pumpkin has been functionally characterized. CmbHLH87 is present in the nucleus. CmbHLH87 expression in the PMresistant material was considerably downregulated by PM; and abscisic acid, methyl jasmonate, ethephon, and NaCl treatments induced CmbHLH87 expression. Ectopic expression of CmbHLH87 in tobacco plants alleviated the PM symptoms on the leaves, accelerated cell necrosis, and enhanced H 2 O 2 accumulation. The expression levels of PR1a, PR5, and NPR1 were higher in the PM-infected transgenic plants than in PMinfected wild-type plants. Additionally, the chlorosis and yellowing of plant materials were less extensive and the concentration of bacteria at infection sites was lower in the transgenic tobacco plants than in the wild-type plants in response to bacterial wilt and scab pathogens. CmbHLH87 may be useful for genetic engineering of novel pumpkin cultivars in the future.

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Section: Discussioncontrasting

confidence: 75%

Expression of Pumpkin CmbHLH87 Gene Improves Powdery Mildew Resistance in Tobacco

et al. 2020

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“…Two lines (TaSTP6-OE1 and TaSTP6-OE2) showing distinct TaSTP6 mRNA accumulation in leaves (Supplemental Fig. S10) were inoculated with powdery mildew (Li et al, 2019), which resulted in an enhanced susceptibility phenotype (Fig. 4A).…”

Section: Overexpression Of Tastp6 Promotes Arabidopsis Susceptibilitymentioning

confidence: 99%

ABA-Induced Sugar Transporter TaSTP6 Promotes Wheat Susceptibility to Stripe Rust

et al. 2019

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Biotrophic pathogens, such as wheat rust fungi, survive on nutrients derived from host cells. Sugar appears to be the major carbon source transferred from host cells to various fungal pathogens; however, the molecular mechanism by which host sugar transporters are manipulated by fungal pathogens for nutrient uptake is poorly understood. TaSTP6, a sugar transporter protein in wheat (Triticum aestivum), was previously shown to exhibit enhanced expression in leaves upon infection by Puccinia striiformis f. sp. tritici (Pst), the causal agent of wheat stripe rust. In this study, we found that Pst infection caused increased accumulation of abscisic acid (ABA) and that application of exogenous ABA significantly enhanced TaSTP6 expression. Moreover, knockdown of TaSTP6 expression by barley stripe mosaic virus-induced gene silencing reduced wheat susceptibility to the Pst pathotype CYR31, suggesting that TaSTP6 expression upregulation contributes to Pst host sugar acquisition. Consistent with this, TaSTP6 overexpression in Arabidopsis (Arabidopsis thaliana) promoted plant susceptibility to powdery mildew and led to increased Glc accumulation in the leaves. Functional complementation assays in Saccharomyces cerevisiae showed that TaSTP6 has broad substrate specificity, indicating that TaSTP6 is an active sugar transporter. Subcellular localization analysis indicated that TaSTP6 localizes to the plasma membrane. Yeast two-hybrid and bimolecular fluorescence complementation experiments revealed that TaSTP6 undergoes oligomerization. Taken together, our results suggest that Pst stimulates ABA biosynthesis in host cells and thereby upregulates TaSTP6 expression, which increases sugar supply and promotes fungal infection.

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“…A previous study on Arabidopsis has shown the involvement of MAPK signaling pathway against PM infection [25]. The KEGG pathway enrichment analysis also indicated that 101 DEGs were enriched in this pathway.…”

Section: B Mapk Signaling Pathway and Toll-like Receptor Signaling Pa...mentioning

confidence: 64%

Phytohormonal and Transcriptomic Response of Hulless Barley Leaf in Response to Powdery Mildew Infection

Sang

Zhang²,

Wang

et al. 2021

Agronomy

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Powdery mildew (PM) caused by Blumeria graminis (DC.) Golovin ex Speer f. sp. hordei Marchal (Bgh) is one of the major yield reducing diseases in hulless barley (Hordeum vulgare L. var. nudum Hook. f.). Genotypes with contrasting resistance to PM offer unique opportunities to explore the transcriptome in order to understand the expression changes in genes and pathways. In this study, we explored the phytohormone levels and transcriptome of a Bgh susceptible (XL19) and resistant (ZYM1288) hulless barley genotypes at 0, 5, 12, 24, and 36 h post infection (hpi) with Bgh. We found relatively higher levels of abscisic acid, jasmonic acid, salicylic acid, and cytokinins in ZYM1288. The transcriptome analyses identified 31,354 genes that were enriched in signaling, energy, and defense related pathways. Higher numbers of differentially expressed genes (DEGs) were found in XL19 as compared to ZYM1288 after 5 (3603 vs. 2341) and 12 hpi (3530 vs. 2416). However, after 24 and 36 hpi, the number of DEGs was higher in ZYM1288 as compared to XL19 i.e., 3625 vs. 3034 and 5855 vs. 2725, respectively. Changes in hormone levels drove downstream expression changes in plant-hormone signaling that helped ZYM1288 to perform better under Bgh infection. The expression of DEGs in MAPK-signaling and Toll-like receptor signaling pathways, glucosinolate biosynthesis, glutathione metabolism, brassinosteroid metabolism, and energy related pathways indicated their common roles in defense against PM. Key genes related to PM-resistance were upregulated in the resistant genotype. These genes provide key information towards differences in both genotypes towards resistance to PM. The transcriptomic signatures explored in this study will broaden our understanding towards molecular regulation of resistance to PM in hulless barley.

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Transcriptome analysis reveals pathways facilitating the growth of tobacco powdery mildew in Arabidopsis

Cited by 6 publications

References 66 publications

Expression of Pumpkin CmbHLH87 Gene Improves Powdery Mildew Resistance in Tobacco

Expression of Pumpkin CmbHLH87 Gene Improves Powdery Mildew Resistance in Tobacco

ABA-Induced Sugar Transporter TaSTP6 Promotes Wheat Susceptibility to Stripe Rust

Phytohormonal and Transcriptomic Response of Hulless Barley Leaf in Response to Powdery Mildew Infection

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