Global analysis of AGO2-bound RNAs reveals that miRNAs induce cleavage of target RNAs with limited complementarity

Jung, Eunsun; Seong, Youngmo; Jeon, Bohyun; Song, Hoseok; Kwon, Young Soo

doi:10.1016/j.bbagrm.2017.10.001

Cited by 11 publications

(14 citation statements)

References 55 publications

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“…The argonaute protein (Ago) family functions as a central factor in RNA silencing processes. It can combine with different classes of small non‐coding RNAs, including miRNAs, to participate in mRNA cleavage or translation inhibition (Go & Mani, ; Jung, Seong, Jeon, Song, & Kwon, ). And a number of circRNAs have been reported to reduce the ability of miRNA to bind to target mRNAs by serving as a binding platform for Ago2 and miRNAs (Hansen et al, ; Memczak et al, ).…”

Section: Introductionmentioning

confidence: 99%

The interaction of circRNAs and RNA binding proteins: An important part of circRNA maintenance and function

Zang

2018

J of Neuroscience Research

457

285

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The widespread expression of circular RNAs (circRNAs) is regarded as a feature of gene expression in highly diverged eukaryotes. Recent studies have shown that circRNAs can act as a miRNA sponge to repress miRNA function, participate in splicing of target genes, translate genes into protein and interact with RNA binding proteins (RBPs). RBPs are a broad class of proteins involved in gene transcription and translation, and interaction with RBPs is considered an important part of circRNA function, which can serve as an essential element underlying the functions of circRNAs, including genesis, translation, transcriptional regulation of target genes, and extracellular transport. In this mini‐review, we attempt to explore in detail the relationship between circRNAs and RBPs, and the interactions between the two factors. The goal of this review is to investigate the emerging studies of RBPs and circRNAs to better understand how their interaction alters cellular function.

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Section: Introductionmentioning

confidence: 99%

The interaction of circRNAs and RNA binding proteins: An important part of circRNA maintenance and function

Zang

2018

J of Neuroscience Research

457

285

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“…On the contrary, siRNAs which are fully complementary to the target, trigger mRNA cleavage by Ago proteins [ 10 ]. Nevertheless, even a near-perfect base pairing of miRNA to its target has been shown to induce mRNA cleavage [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

The RNA–RNA base pairing potential of human Dicer and Ago2 proteins

Pokornowska

Milewski

Ciechanowska

et al. 2019

Cell. Mol. Life Sci.

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The ribonuclease Dicer produces microRNAs (miRNAs) and small interfering RNAs that are handed over to Ago proteins to control gene expression by targeting complementary sequences within transcripts. Interestingly, a growing number of reports have demonstrated that the activity of Dicer may extend beyond the biogenesis of small regulatory RNAs. Among them, a report from our latest studies revealed that human Dicer facilitates base pairing of complementary sequences present in two nucleic acids, thus acting as a nucleic acid annealer. Accordingly, in this manuscript, we address how RNA structure influences the annealing activity of human Dicer. We show that Dicer supports hybridization between a small RNA and a complementary sequence of a longer RNA in vitro, even when both complementary sequences are trapped within secondary structures. Moreover, we show that under applied conditions, human Ago2, a core component of RNA-induced silencing complex, displays very limited annealing activity. Based on the available data from new-generation sequencing experiments regarding the RNA pool bound to Dicer in vivo, we show that multiple Dicer-binding sites within mRNAs also contain miRNA targets. Subsequently, we demonstrate in vitro that Dicer but not Ago2 can anneal miRNA to its target present within mRNA. We hypothesize that not all miRNA duplexes are handed over to Ago proteins. Instead, miRNA-Dicer complexes could target specific sequences within transcripts and either compete or cooperate for binding sites with miRNA-Ago complexes. Thus, not only Ago but also Dicer might be directly involved in the posttranscriptional control of gene expression.

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“…The argonaute 2 (AGO2) protein family is a pivotal component of the RNA silencing complex and plays a prominent role in cancer aggressiveness (Jung et al, 2017; Meister et al, 2004). Studies revealed that circAGO2 (hsa_circ_0135889), consisting of the first intron of AGO2, is upregulated in esophageal cancer, gastric cancer (GC), colorectal cancer (CRC) and PCa, suggesting a compelling role of tumorigenesis and development (Papachristou et al, 2011; Yoo et al, 2010; Zhang et al, 2013).…”

Section: Circrna Binds To Rbps and Regulates Cancer Progressionmentioning

confidence: 99%

“…The argonaute 2 (AGO2) protein family is a pivotal component of the RNA silencing complex and plays a prominent role in cancer aggressiveness (Jung et al, 2017;Meister et al, 2004).…”

Section: Circrna Binds To Rbps and Regulates Cancer Progressionmentioning

confidence: 99%

Regulation of cancer progression by circRNA and functional proteins

Chen

Tang

et al. 2021

Journal Cellular Physiology

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Circular RNAs (circRNAs) are closed back‐splicing products of precursor mRNA in eukaryotes. Compared with linear mRNAs, circRNAs have a special structure and stable expression. A large number of studies have provided different regulatory mechanisms of circRNAs in tumors. Challenges exist in understanding the control of circRNAs because of their sequence overlap with linear mRNA. Here, we survey the most recent progress regarding the regulation of circRNA biogenesis by RNA‐binding proteins, one of the vital functional proteins. Furthermore, substantial circRNAs exert compelling biological roles by acting as protein sponges, by being translated themselves or regulating posttranslational modifications of proteins. This review will help further explore more types of functional proteins that interact with circRNA in cancer and reveal other unknown mechanisms of circRNA regulation.

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Global analysis of AGO2-bound RNAs reveals that miRNAs induce cleavage of target RNAs with limited complementarity

Cited by 11 publications

References 55 publications

The interaction of circRNAs and RNA binding proteins: An important part of circRNA maintenance and function

The interaction of circRNAs and RNA binding proteins: An important part of circRNA maintenance and function

The RNA–RNA base pairing potential of human Dicer and Ago2 proteins

Regulation of cancer progression by circRNA and functional proteins

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