Siccirubricoccus deserti gen. nov., sp. nov., a proteobacterium isolated from a desert sample

Abstract

Strain SYSU D8009 was isolated from a desert sample collected from Saudi Arabia. The taxonomic position of the isolate was investigated by a polyphasic approach. The novel isolate was Gram-stain-negative, non-motile, aerobic and non-spore-forming. It was able to grow at 4-45 °C and pH 4.0-8.0, and exhibited NaCl tolerance of up to 1.5 % (w/v). Strain SYSU D8009 shared the closest 16S rRNA gene sequence similarities with members of the family Acetobacteraceae, with a value of less than 96.0 %. In the phylogenetic dendrograms, the strain clustered with the genera Paracraurococcus, Craurococcus and Crenalkalicoccus within the family Acetobacteraceae but with a distinct lineage, thereby demonstrating that the strain should be classified within the family Acetobacteraceae. The respiratory ubiquinone was found to be Q-10. The polar lipids of the strain comprised diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and four unidentified aminolipids. The predominant cellular fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C16 : 0. The genomic DNA G+C content of strain SYSU D8009 was determined to be 71.6 mol%. Based on the results of the phylogenetic analyses and differences in the physiological and biochemical characteristics, strain SYSU D8009 merits representation of a novel species of a new genus within the family Acetobacteraceae, for which the name Siccirubricoccus deserti gen. nov., sp. nov. is proposed. The type strain of Siccirubricoccus deserti sp. nov. is SYSU D8009 (=CGMCC 1.15936=KCTC 62088).

Vitreimonas flagellata gen. nov., sp. nov., a novel member of the family Hyphomonadaceae isolated from an activated sludge sample

“…[12]. Amplification of the 16S rRNA gene, purification, cloning, sequencing and assembly of the raw sequences were done as described earlier [13]. The determined clone sequence was submitted to the EzBioCloud server [14] to determine the closely related species.…”

Agilicoccus flavus gen. nov., sp. nov., a novel member of the family Dermatophilaceae isolated from the Pearl River

“…Genomic DNA of strain SYSU M44304 T was extracted as previously described [16]. Amplification of the 16S rRNA gene, purification, cloning, sequencing and assembly of the raw sequence data were performed as described earlier [17]. The sequence obtained was compared with the 16S rRNA gene sequences of species with validly published names in the EzBioCloud server database [18].…”

“…Genomic DNA of strain SYSU M44304 T was extracted as previously described [16]. Amplification of the 16S rRNA gene, purification, cloning, sequencing and assembly of the raw sequence data were performed as described earlier [17].…”

Roseomonas ponticola sp. nov., a novel bacterium isolated from Pearl River estuary

“…Genomic DNA of strain SYSU M41301 T was extracted as previously described [14]. Amplification of the 16S rRNA gene, purification, cloning, sequencing and assembly of the raw sequences were done as described earlier [15]. The sequences obtained were compared with the 16S rRNA gene sequences of species with validly published names in EzBioCloud server database [16].…”