Vitreimonas flagellata gen. nov., sp. nov., a novel member of the family Hyphomonadaceae isolated from an activated sludge sample

Asem, Mipeshwaree Devi; Salam, Nimaichand; Zheng, Wei; Liu, Lihua; Zhang, Xiaotong; Han, Ming-Xian; Jiao, Jian‐Yu; Guo, Feng; Li, Wenjun

doi:10.1099/ijsem.0.004082

“…The fatty acid profile was composed of iso-C 16 : 0 (28.2 %), C 18 : 0 10-methyl TBSA (20.1 %), anteiso-C 17 : 0 (16.1 %), iso-C 18 : 0 (6.9 %), C 18 : 0 (6.7 %), C 17 : 0 10-methyl (5.8 %), C 12 : 0 (2.4 %), C 18 : 1 ω8c (1.6 %), C 17 : 0 (1.6 %), summed feature 9 (C 16 : 0 10-methyl and/or iso-C 17 : 1 ω9c, 1.5 %), C 16 : 0 (1.5 %), iso-C 14 : 0 (1.1 %), anteiso-C 15 : 0 (1.1 %) and minor contents (<1 %) of iso-C 10 : 0 , iso-C 12 : 0 , anteiso-C 13 : 0 , iso-C 15 : 0 , C 14 : 0 2OH, iso-C 17 : 0 , C 17 : 1 ω8c, anteiso-C 19 : 0 , summed feature 7 (C 19 : 1 ω7c and/or C 19 : 1 ω6c), C 19 : 0 , C 18 : 1 2OH and iso-C 20 : 0 . Growth at 4, 15, 20, 28, 37, 45, 55, 60 and 65 °C and from pH 4 to 13.0 (at intervals of 1.0 pH unit) were observed by culturing in TSB for 7 and 14 days; the pH of the media were maintained as described by Asem et al [33]. Salt tolerance was observed by supplementing TSA with up to 20 % (w/v) NaCl.…”

mentioning

confidence: 99%

Nocardiopsis deserti sp. nov., isolated from a high altitude Atacama Desert soil

Asem

¹

,

Salam

²

,

Idris

³

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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The taxonomic status of a Nocardiopsis strain, designated H13T, isolated from a high altitude Atacama Desert soil, was established by using a polyphasic approach. The strain was found to have chemotaxonomic, cultural and morphological characteristics consistent with its classification within the genus Nocardiopsis and formed a well-supported clade in the Nocardiopsis phylogenomic tree together with the type strains of Nocardiopsis alborubida , Nocardiopsis dassonvillei and Nocardiopsis synnematoformans. Strain H13T was distinguished from its closest relatives by low average nucleotide identity (93.2–94.9 %) and in silico DNA–DNA hybridization (52.5–62.4 %) values calculated from draft genome assemblies and by a range of phenotypic properties. On the basis of these results, it is proposed that the isolate be assigned to the genus Nocardiopsis as Nocardiopsis deserti sp. nov. with isolate H13T (=CGMCC 4.7585T=KCTC 49249T) as the type strain.

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“…The almost-complete 16S rRNA gene sequence of strain SZ9 T (1430 bp; GenBank accession number MZ092877), which was consistent with that of the whole genome, had highest similarity values to Aquidulcibacter paucihalophilus TH1-2 T (92.44 %) [16], followed by members of other genera: Vitreimonas flagellata SYSU XM001 T (89.61 %) [17], Asprobacter aquaticus DRW22-8 T (89.49 %) [18] and Hyphobacterium vulgare WM6 T (89.49 %) [19]. The sequence similarity values of all type strains available in GenBank to strain SZ9 T were less than 93 %, which was far below the threshold of 95 % for genus delineation, indicating that strain SZ9 T represented a novel genus [20].…”

Section: Resultsmentioning

confidence: 83%

“…Differential characteristics of strain SZ9 T and related type strains within the family Caulobacteraceae and Hyphomonadaceae Taxa: 1, strain SZ9 T (this study); 2, Aquidulcibacter [16]; 3. Terricaulis [22]; 4, Phenylobacterium [23][24][25][26]; 5, Vitreimonas [17]; 6, Asprobacter [18]; 7, Hirschia [27,28]; 8, Maribaculum [29]; 9, Ponticaulis [30,31]; 10, Hyphomonas [32,33]; 11, Henriciella [34][35][36]. +, Positive; w, weakly positive; −, negative; nd, no data.…”

Section: Morphological Physiological and Biochemical Characteristicsmentioning

confidence: 99%

“…Cellular fatty acid compositions (>1.0 %) of strain SZ9 T and related type strains within the family Caulobacteraceae and Hyphomonadaceae Strain: 1, SZ9 T (from this study); 2, Aquidulcibacter paucihalophilus TH1-2 T [16]; 3, Terricaulis silvestris 0127_4 T[22]; 4, Vitreimonas flagellata SYSU XM001 T[17]; 5, Asprobacter aquaticus DRW22-8 T[18]; 6, Hyphobacterium vulgare WM6 T[19]. Values are percentages of total fatty acids.…”

mentioning

confidence: 99%

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Pseudaquidulcibacter saccharophilus gen. nov., sp. nov., a novel member of family Caulobacteraceae, isolated from a water purification facility with supplement of starch as a carbon source

Liu

¹

,

Li

²

,

Lin

³

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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During a survey of microbial communities in the influent (ambient water) and effluent of a water purification facility with aeration and supplement of starch as carbon source, a novel bacterial strain, designated SZ9T, was isolated from the effluent sample. Colonies of strain SZ9T were small (approximately 0.5–1.0 mm in diameter), creamy-white, circular, smooth, translucent and convex. Cells were facultative anaerobic, motile by means of a single polar flagellum, rod-shaped, multiplied by binary fission, Gram-stain-negative, oxidase-positive and catalase-negative. Growth occurred at 10–40 °C (optimum, 28 °C) and pH 5.5–8.0 (optimum, pH 7.5). The range of NaCl concentration for growth was 0–1.0 % (w/v), with an optimum of 0–0.5 % (w/v). Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain SZ9T formed a lineage within the family Caulobacteraceae of the class Alphaproteobacteria and showed the highest 16S rRNA gene sequence similarities to Aquidulcibacter paucihalophilus TH1-2T (92.44%), followed by Vitreimonas flagellata SYSU XM001T (89.61 %), Asprobacter aquaticus DRW22-8T (89.49 %) and Hyphobacterium vulgare WM6T (89.49%). The predominant fatty acids (>10 % of the total fatty acids) of strain SZ9T was summed feature 3 (comprising C16 : 1 ω6c and/or C16 : 1 ω7c), summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c) and C16 : 0. The sole respiratory quinone was ubiquinone-10, and the major polar lipids were phosphatidylcholine and two unidentified glycolipids. The whole genome of strain SZ9T was 2 842 140 bp in size, including 2769 protein-coding genes, 37 tRNA genes and two rRNA genes, and the genomic G+C content was 41.4 mol%. The orthologous average nucleotide identity, average amino acid identity and digital DNA–DNA hybridization values between strain SZ9T and other genera within the family Caulobacteraceae were 64.50–66.62 %, 46.96–54.17 % and 27.70–31.70 %, respectively. Therefore, based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, the isolated strain SZ9T could be distinguished from other genera, suggesting that it represents a novel species of a novel genus in the family Caulobacteraceae , for which the name Pseudaquidulcibacter saccharophilus gen. nov., sp. nov is proposed. The type strain is SZ9T (=CCTCC AB2021029T=KCTC 82788T).

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“…A relevant emendation is given below. Also, we were unable to confirm that Vitreimonas flagellata belonged to the Hyphomonadaceae cluster [56]. Our extended 16S rRNA tree (Fig.…”

Section: Full-textmentioning

confidence: 96%

Alkalicaulis satelles gen. nov., sp. nov., a novel haloalkaliphile isolated from a laboratory culture cyanobacterium Geitlerinema species and proposals of Maricaulaceae fam. nov., Robiginitomaculaceae fam. nov., Maricaulales ord. nov. and Hyphomonadales ord. nov.

Kevbrin

¹

,

Boltyanskaya

²

,

Koziaeva

³

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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A prosthecate bacterial strain, designated G-192T, was isolated from decaying biomass of a haloalkaliphilic cyanobacterium Geitlerinema sp. Z-T0701. The cells were aerobic, Gram-negative, non-endospore-forming and dimorphic, occurring either as sessile bacteria with a characteristic stalk or as motile flagellated cells. The strain utilized a limited range of substrates, mostly peptonaceous, but was able to degrade whole proteins. Growth occurred at 5–46 °C (optimum, 35–40 °C), pH 7.3–10.3 (optimum, pH 8.0–9.0), 0–14 % NaCl (v/w; optimum, 2.0–6.0 %, v/w). The G+C content of the genomic DNA of strain G-192T was 66.8%. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain G-192T formed a distinct evolutionary lineage within the family Hyphomonadaceae . Strain G-192T showed the highest 16S rRNA sequence similarity to Glycocaulis profundi ZYF765T (95.2%), Oceanicaulis stylophorae GISW-4T (94.2%) and Marinicauda salina WD6-1T (95.5%). The major cellular fatty acids (>5% of the total) were C18:1 ω9c, C18:0 and 11-methyl-C18:1 ω7c. The major polar lipids were glycolipids and phospholipids. The only respiratory quinone was ubiquinone-10 (Q-10). Based on polyphasic results including phylogenomic data, the novel strain could be distinguished from other genera, which suggests that strain G-192T represents a novel species of a new genus, for which the name Alkalicaulis satelles gen. nov., sp. nov. is proposed. The type strain is G-192T (=VKM B-3306T=KCTC 72746T). The strain is the first representative of the stalked bacteria associated with a haloalkaliphilic cyanobacterium. Based on phylogenomic indices and phenotypic data, it is proposed to evolve two novel families Maricaulaceae fam. nov. and Robiginitomaculaceae fam. nov. out of the current family Hyphomonadaceae . In addition, it is proposed to place the first two families in the novel order Maricaulales ord. nov. and novel order Hyphomonadales ord. nov. is proposed to accommodate the family Hyphomonadaceae .

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Vitreimonas flagellata gen. nov., sp. nov., a novel member of the family Hyphomonadaceae isolated from an activated sludge sample

Cited by 10 publications

References 51 publications

Nocardiopsis deserti sp. nov., isolated from a high altitude Atacama Desert soil

Nocardiopsis deserti sp. nov., isolated from a high altitude Atacama Desert soil

Pseudaquidulcibacter saccharophilus gen. nov., sp. nov., a novel member of family Caulobacteraceae, isolated from a water purification facility with supplement of starch as a carbon source

Alkalicaulis satelles gen. nov., sp. nov., a novel haloalkaliphile isolated from a laboratory culture cyanobacterium Geitlerinema species and proposals of Maricaulaceae fam. nov., Robiginitomaculaceae fam. nov., Maricaulales ord. nov. and Hyphomonadales ord. nov.

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