Determination of p16 overexpression as an indicator of human papillomavirus infection in oral dysplasia and carcinoma

Pathak, Adrija; Singh, Mahendra; Agarwal, Asha; Amit, Sonal

doi:10.4103/ijdr.ijdr_79_15

Cited by 7 publications

(7 citation statements)

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“…In line with this, we found that p16 was more often overexpressed in OL with dysplasia. The sensitivity was 29% and the specificity 84%, which differs from the 83% sensitivity and 40% specificity reported by Pathak et al (50).…”

Section: Discussioncontrasting

confidence: 81%

Combined Testing of p16 Tumour-suppressor Protein and Human Papillomavirus in Patients With Oral Leukoplakia and Oral Squamous Cell Carcinoma

et al. 2019

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Background: Oral leukoplakia (OL) is a potentially malignant oral mucosal disorder. A casual association between OL, oral squamous cell carcinoma (OSCC) and human papillomavirus (HPV) infection has been suggested, but no conclusive evidence has been presented. p16, a tumoursuppressor protein, is used as a surrogate marker for HPV infection. The aim of this study was to investigate how overexpression of p16 correlates with HPV infection in OL and in OSCC. Patients and Methods: Seventy-four patients with OL and 13 with OSCC with p16 overexpressed, were analyzed by immunohistochemistry visualizing p16 and a real-time polymerase chain reaction (

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Section: Discussioncontrasting

confidence: 81%

Combined Testing of p16 Tumour-suppressor Protein and Human Papillomavirus in Patients With Oral Leukoplakia and Oral Squamous Cell Carcinoma

et al. 2019

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“…p16 INK4a expression detection by IHC was performed in five serial sections of 5 μm FFPE tissue as per Klaes et al and detection was performed as per BioGenex manufacturer's protocol (Catalog # QD400, BioGenex, CA). All the sections showing either strong nuclear and/or cytoplasmic immunostaining were considered positive and graded qualitatively according to the following arbitrary scale: 0 (no positive staining), +1 (<10% cells showing positive staining), +2 (>10% but <50% positive staining), and +3 (>50% positive staining) …”

Section: Methodsmentioning

confidence: 99%

“…Of several genotypes, HPV 16 is the most prevalent subtype of HPV, commonly affects the younger age groups with male predisposition . p16 immunostaining has been considered as the first‐line assay in the detection of HPV‐induced cancers . However, polymerase chain reaction (PCR) amplification has been considered as the gold standard technique in the detection of HPV DNA in OSCC cases; it offers advantages such as high sensitivity and type‐specific and formalin‐fixed paraffin‐embedded (FFPE) tissue acceptances .…”

Section: Introductionmentioning

confidence: 99%

“…6 p16 immunostaining has been considered as the first-line assay in the detection of HPV-induced cancers. 7,8 However, polymerase chain reaction (PCR) amplification has been considered as the gold standard technique in the detection of HPV DNA in OSCC cases; it offers advantages such as high sensitivity and typespecific and formalin-fixed paraffin-embedded (FFPE) tissue acceptances. 9 However, HPV DNA alone is insufficient evidence to drive a causal association from a molecular perspective.…”

Section: Introductionmentioning

confidence: 99%

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Quantitative polymerase chain reaction‐based detection of HPV 16 E6 and E7 DNA in oral squamous cell carcinoma

Thammaiah¹,

Venkobarao

Hemavathy

et al. 2018

J Oral Pathology Medicine

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“…(13) Several studies have validated high degree of association of HPV infection and p16 expression suggesting that p16 can be used as a surrogate marker for high risk HPV in oral squamous cell carcinomas. (14)(15)(16) In this study, we aimed to determine the prevalence of HPV in patients with oral and oropharyngeal carcinoma at a tertiary care hospital in Central India using p-16 as a surrogate marker of high-risk HPV infection. Additionally, association of p-16 immunoexpression with clinicopathological characteristics, namely, age, sex and histological type/grade of cancer was established.…”

mentioning

confidence: 99%

PREVALENCE OF HUMAN PAPILLOMA VIRUS IN A TERTIARY CARE CENTRE IN CENTRAL INDIA USING p16 IMMUNOEXPRESSION AS A SURROGATE MARKER

Yadav¹,

Tandon²

2019

jemds

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BACKGROUND High risk Human Papilloma Virus (HR-HPV) has now emerged as one of the major etiological factors in oral and oropharyngeal cancer. Knowing HPV status is crucial as it has a significant impact on therapeutic front as well as on prognosis. As p16 immunoexpression can serve as surrogate marker for HR-HPV infection, in the current study we used p16 immunoexpression to determine the prevalence of HPV in a tertiary care centre in Central India. MATERIALS AND METHODSThis is hospital based cross sectional study conducted in oral biopsy tissues (for neoplastic lesions) received for histopathological evaluation over a period of one and half years. The sections were processed for H & E staining, and 112 cases were chosen for immunohistochemical study. The data was analysed by chi-square and Z-tests using software SPSS. RESULTSPositive diffuse p16 immunoexpression was present in 23.2% of the cases irrespective of histological type/grade of the lesion. We found significant correlation between degree of dysplasia and p16 immunoexpression with 16.7% of cases showing positivity in OIN I cases as compared to 25% in OIN II and 77.8 % in OIN III. Poorly differentiated tumours had high proportion of cases with positive p16 immunoexpression (75%) compared to well differentiated (29.3%) and moderately differentiated squamous cell carcinomas (35.5%). Additionally, higher proportion of female patients (43.8%) had diffuse p16 positivity than males (17%). CONCLUSIONWe conclude that HPV prevalence in our tertiary care centre is approximately 23.2%. This subset of cases was found to be associated more with oropharyngeal lesions and was present in higher proportions in female patients. Apart from serving as a surrogate marker for HR-HPV, p16 immunoexpression also correlates with the degree of dysplasia in oral and oropharyngeal lesions.

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Determination of p16 overexpression as an indicator of human papillomavirus infection in oral dysplasia and carcinoma

Abstract: p16 immunostaining is a good first-line assay for eliminating HPV-negative cases from additional analysis, but other causes of p16 overexpression in oral tumorigenesis related to tobacco consumption in keratinizing squamous cell carcinoma needs to be explored further.

Cited by 7 publications

References 0 publications

Combined Testing of p16 Tumour-suppressor Protein and Human Papillomavirus in Patients With Oral Leukoplakia and Oral Squamous Cell Carcinoma

Combined Testing of p16 Tumour-suppressor Protein and Human Papillomavirus in Patients With Oral Leukoplakia and Oral Squamous Cell Carcinoma

Quantitative polymerase chain reaction‐based detection of HPV 16 E6 and E7 DNA in oral squamous cell carcinoma

PREVALENCE OF HUMAN PAPILLOMA VIRUS IN A TERTIARY CARE CENTRE IN CENTRAL INDIA USING p16 IMMUNOEXPRESSION AS A SURROGATE MARKER

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