Modulatory effect of lycopene against carbofuran toxicity in African catfish, Clarias gariepinus

Abstract

In the current study, African catfish, Clarias gariepinus, was exposed to a sublethal concentration of carbofuran (CF) to investigate its negative effects on biochemical and oxidative stress biomarkers. Also, the putative role of lycopene (LYC) administration in alleviating these negative effects was evaluated. Fish were divided into six groups in triplicates as follows: group I was without treatment, group II was orally administered corn oil, group III was orally administered 18 mg LYC/kg body weight, group IV was exposed to 0.121 mg CF/L, group V was orally administered 9 mg LYC/kg body weight and exposed to 0.121 mg CF/L, and group VI was orally administered 18 mg LYC/kg body weight and exposed to 0.121 mg CF/L for 4 weeks. At the end of this period, blood and tissue (liver and kidney) samples were collected and biochemical and oxidative stress biomarkers were analysed. Also, histopathological changes were determined. Carbofuran caused significant increments of glucose, cortisol, aspartic amino transferase, alanine amino transferase, cholesterol, urea, and creatinine; meanwhile, serum acetylcholinesterase, total protein, albumin, and total lipids were significantly reduced. Significant increments in hepatic and renal malondialdehyde (MDA) and superoxide dismutase (SOD) levels and marked reduction in hepatic and renal catalase (CAT), glutathione (GSH), and total antioxidant capacity (TAC) levels were observed in CF-exposed fish comparing to the control group. Treatment with LYC attenuated the CF-induced oxidative stress, and this improvement was more pronounced in fish received the high LYC dose (18 mg/kg body weight). Further, congestion of the central vein with infiltration of mononuclear inflammatory cells, vacuolar necrosis, and haemorrhage was observed in the livers of CF-exposed fish. Oral administration of LYC reduced behavioural changes and histopathological alterations. All the altered biochemical parameters and antioxidant biomarkers were also restored to be near the normal levels. The obtained results evoked that LYC administration alleviated the destructive effects of carbofuran and reduced its toxicity effect on African catfish.

Lycopene reduces the impacts of aquatic environmental pollutants and physical stressors in fish

“…African catfish were exposed to 0.121 mg CF L −1 and fed lycopene at a level of 18 mg and 9 mg per kg body weight for 1 month (Hamed & Osman, 2017). The results revealed that fish fed on 18 mg lycopene per kg body weight showed a pronounced alleviation of oxidative stress induced by CF via boosting the activities of CAT and SOD and reducing the MDA, GSH and TAC levels.…”

“…It can alleviate the oxidative stress resulted from exposure of fish to sublethal levels of cadmium chloride (Mekkawy et al, 2011; Mekkawy et al, 2012), diazinon (DZN) (Ibrahim & Banaee, 2014; Ibrahim, 2015), zinc oxide nanoparticles (ZnONPs) (Abdel‐Daim et al, 2019), and endosulfan toxicity (Hussein et al, 2019). Lycopene also improved the hepatorenal functions and the haematological picture and reduced the serum oxidative stress biomarkers in African catfish ( Clarias gariepinus ) exposed to lead nitrate toxicity (Mahmoud et al, 2013), sublethal doses of atrazine (Mekkawy et al, 2013) and carbofuran (CF) (Hamed & Osman, 2017). Lycopene attenuates the oxidative stress responses and haemato‐immunological alterations of common carp ( Cyprinus carpio ) exposed to deltamethrin (DLM) (Yonar & Sakin, 2011), chlorpyrifos (CPF) (Ural, 2013), cypermethrin (CYP) (Yonar, 2013a), malathion (Yonar, 2013b) and trichlorfon (Yonar et al, 2020).…”

Antagonistic effects of dietaryMoringa oleiferaon hemato‐biochemical and oxidative stress of lead nitrate intoxicated Nile tilapia,Oreochromis niloticus

“…Studies have shown that plants having medicinal properties can be used for mitigating metal toxicity (Abdel-Tawwab & Hamed, 2020;Hamed & Abdel-Tawwab, 2021). Similar studies also revealed that several plant-derived or chitosan-derived nanocomposite application in fish culture could enhance the survivability and safeguard from several water pollutants such as heavy metals, carbofuran Hamed & Osman, 2017), bisphenol-A (Hamed & Abdel-Tawwab, 2017;Hussein et al, 2021), ochratoxin-A (Abdel-Wahhab et al, 2016), etc.…”

“…AST and ALT are primarily liver enzymes associated with the metabolism of protein and amino acids. An elevated level of these enzymes in serum indicates liver damage (Hamed & Osman, 2017).…”

Состояние биомониторного вида черноморской скорпены (Scorpaena Porcus Linnaeus, 1758) в прибрежных акваториях Севастополя

“…2). Следует указать, что в экспериментальных условиях, при кратковременных токсических нагрузках на рыб, другие исследователи наблюдали противоположный эффект [Hamed, Osman, 2017]. Для бухты Балаклавской повышенные величины изученных биохимических маркеров объясняются отличным от других акваторий рационом скорпены [Papoutsoglou, Papaparaskeva-Papoutsoglou, 1979].…”