Analysis of the association of clinical factors and IL4 gene polymorphisms with root resorption in avulsed teeth after 1 year of replantation

Abstract

Roskamp L, Trevilatto PC, Souza CM, Silva Neto UX, Carneiro E, Fariniuk LF, Westphalen VPD. Aim To investigate the association of clinical variables and polymorphisms (tag SNPs) in the interleukin 4 (IL4) gene, with the prognosis of avulsed and replanted teeth. Methodology Ninety-four patients who suffered avulsion and had their teeth replanted and endodontically treated were included. Periapical radiographs were obtained soon after tooth replantation and after 1 year. For genotypic IL4 gene analysis, the DNA from oral mucosa cells was collected, and polymorphisms were investigated by real-time PCR. Univariate and multivariate analyses were performed to verify the association of clinical and genetic variables and the outcome of the replanted teeth (P < 0.05).Results After multivariate analysis, extra-alveolar time longer than 1 h was significantly associated with external root resorption. No significant association was observed between IL4 gene polymorphisms and root resorption. Conclusion No association between root resorption and IL4 gene polymorphisms was observed. An extraalveolar time of more than 1 h was associated with a susceptibility for external root resorption. Replanting the tooth in its socket immediately is the most important factor to maintain a healthy root surface.

The effect of soy and whole milk as a means to store avulsed teeth: A histometric study

“…It does not contain cholesterol or lactose and has little saturated fat 23,30 . Whole milk contains carbohydrates, proteins, fats, calcium, vitamins, and growth factors 4,5,32 . The similar composition of both substances may be the variable that has provided a similar impact on cell repair, making soy milk a potential storage medium, similar to whole milk.…”

“…In these cases, the use of an adequate storage medium is extremely important for cell preservation and for the prognosis of the tooth, because when the tooth is stored in an appropriate medium, it can be kept longer, extending the extra‐alveolar time. In contrast, with dry storage, time is crucial for cell preservation, and the extra‐alveolar time should be as short as possible 4‐6 …”

“…In contrast, with dry storage, time is crucial for cell preservation, and the extra-alveolar time should be as short as possible. [4][5][6] Studies have shown that the ideal storage vehicle should be a substance easily accessible at the time of trauma. It should also preserve cell viability, increase the osteoclastogenic potential of the periodontal ligament cells, 7 possess fixation capacity and cell clonogenicity, 8,9 in addition to having physiologically compatible pH and osmolarity.…”

Anti‐inflammatory and anti‐resorptive efficacy of adrenergic blockers on late replanted rat incisors

“…Therefore, the hard dental tissues (cementum and dentin) will be incorporated into the continuous physiological remodeling process. Unlike bone tissue that is constantly remodeled and undergoes a process of resorption and deposition, the roots of permanent teeth do not have this homeostatic mechanism, and if the process of resorption is initiated, it can lead to the total loss of the root . It is estimated that 60 to 85% of replanted teeth are resorbed and lost 4‐6 years after injury .…”

The role of polymorphism of the IL6 gene in tooth replantation

“…The genetic factors that predispose patients to hard tissue resorption have been investigated in the fields of orthodontics, periodontics and endodontics (14–16). Several studies have investigated the association between the genetic polymorphisms of IL‐1, IL‐4 and external root resorption (15,17–19). However, this association was not established in apical periodontitis repair or in external root resorption, where the participation of bacteria occurs as an additional stimulus to the inflammatory response (17).…”