The effect of soy and whole milk as a means to store avulsed teeth: A histometric study

Abstract

Background/Aim: An adequate storage medium can help preserve the viability of periodontal ligament cells, favoring the periodontal ligament repair process. The aim of this study was to evaluate the repair process following the delayed replantation of teeth stored in soy milk and whole milk. Material and Methods: The maxillary right incisors of 27 rats were divided into three equally sized groups: Group SM (delayed replantation with storage in soy milk), whose teeth were immersed in 50 mL of soy milk for 60 minutes; Group WM (delayed replantation with whole milk), teeth were kept for 60 minutes in 50 mL of whole milk; and Group D (delayed replantation with dry storage), teeth were kept in dry conditions for the same period (negative control). Next, the teeth of the animals of all groups were replanted into their sockets. Systemic antibiotics were given to all the animals, and then, they were euthanized after 60 days. Sections were obtained and stained with hematoxylin and eosin for histomorphometric study. The presence and location of acute and chronic inflammatory processes in the epithelial insertion of the protective and insertion periodontium were evaluated, as well as the presence, extension, depth, and repair of root resorption, and the resorption and ankylosis with bone. Statistical differences between groups were analyzed using the Dunn test. Results: Groups SM and WM showed similar patterns in the repair of the periodontal ligament following delayed replantation, in relation to the inflammatory process in the proximities of the epithelial insertion, inflammation and organization of the periodontal ligament, plus root and bone resorption. However, in group WM, more ankylosis was observed than in group SM. Conclusion: The teeth stored in soy milk and whole milk had similar periodontal repair, which shows the potential of soy milk as a storage medium before tooth replantation.

Knowledge and Attitude of Guardians in Management of Avulsion of Primary and Permanent Teeth of Children: A Questionnaire-Based Study

“…Milk (27.3%) and Hanks Balanced Salt Solution (24.7%) were the commonly chosen options. These results correspond with the studies in the literature which reports similar findings as milk being commonly used transport medium [15], [16]. Milk is available nearly in every household due to its low cost, high rate of consumption, and easy availability along with its properties to preserve PDL cells viability [17].…”

Evaluation of the Efficacy of Propolis as Storage Media for Avulsed Teeth

“…Propolis has certain characteristics that encouraged its testing as an effective storage media as it is rich in flavonoids, the most essential pharmacologically active element and potent antioxidant in propolis which explains its potential to maintain cell viability (11)(12)(13) . (15) reported that milk is the most recommended physiologic storage solution for an avulsed tooth so it was selected as a comparison medium in this study.…”

Effect of Di̇fferent Avulsed Tooth Storage Medi̇a on i̇n Vi̇tro Cell Vi̇abi̇li̇ty of Human Peri̇odontal Li̇gament Fi̇broblasts

“…In the study by Oikarinen and Seppā, no difference was found between milk and saliva after a 60 minute incubation. [9][10][11] The reason can be explained by the proliferation capacity of PDL cells, which does not decrease more significantly in saliva than in milk during shorter incubation periods. In this study, milk can be suggested as a transport medium for short-term preservation because of its low bacterial content, physiological osmolarity, neutral pH, and nutrients and factors that will ensure PDL cell viability.…”