Preparation of micro-porous bioceramic containing silicon-substituted hydroxyapatite and beta-tricalcium phosphate

Fuh, Lih‐Jyh; Huang, Yajing; Chen, Wen‐Cheng; Lin, Dao‐Hong

doi:10.1016/j.msec.2017.02.065

Cited by 36 publications

(8 citation statements)

References 47 publications

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“… 16 On the other hand, the antibacterial capability of HAp is another significant property for bone grafts and dental implant applications to prevent infections after surgery. 17 Doping with metal ions is the most powerful strategy to improve the antibacterial activity of HAp. 18 Several metal doping ions had been used in the previous reports to enhance the antibacterial activity of HAp, such as silver (Ag + ), zinc (Zn 2+ ), magnesium (Mg 2+ ), and copper (Cu 2+ ) ions.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and characterizations of natural limestone-derived nano-hydroxyapatite (HAp): a comparison study of different metals doped HAps on antibacterial activity

et al. 2021

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Section: Introductionmentioning

confidence: 99%

Synthesis and characterizations of natural limestone-derived nano-hydroxyapatite (HAp): a comparison study of different metals doped HAps on antibacterial activity

et al. 2021

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“…The use of Si and its result as a stabilizing agent in calcium phosphates (CaPs) has been well documented [27,28]. XRD results shown in Figure 1 confirm that α-TCP phase formation is significantly reduced in samples containing Si and Zn.…”

Section: Discussionmentioning

confidence: 73%

Regulation of Osteogenic Markers at Late Stage of Osteoblast Differentiation in Silicon and Zinc Doped Porous TCP

Fielding

Sarkar

Vahabzadeh

et al. 2019

JFB

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Calcium phosphates (CaPs) are one of the most widely used synthetic materials for bone grafting applications in the orthopedic industry. Recent trends in synthetic bone graft applications have shifted towards the incorporation of metal trace elements that extend the performance of CaPs to have osteoinductive properties. The objective of this study is to investigate the effects of silicon (Si) and zinc (Zn) dopants in highly porous tricalcium phosphate (TCP) scaffolds on late-stage osteoblast cell differentiation markers. In this study, an oil emulsion method is utilized to fabricate highly porous SiO2 doped β-TCP (Si-TCP) and ZnO doped β-TCP (Zn-TCP) scaffolds through the incorporation of 0.5 wt.% SiO2 and 0.25 wt.% ZnO, respectively, to the β-TCP scaffold. Reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) is utilized to analyze the mRNA expression of osteoprotegerin (OPG), receptor activator of nuclear kappa beta ligand (RANKL), bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (Runx2) at the later stage of osteoblast differentiation, day 21 and day 28. Results show that the addition of Si and Zn to the β-TCP structure inhibited the β to α-TCP phase transformation and enhance the density without affecting the dissolution properties. Normal BMP-2 and Runx2 transcriptions are observed in both Si-TCP and Zn-TCP scaffolds at the initial time point, as demonstrated by RT-qPCR. Moreover, the addition of both Si and Zn positively regulate the osteoprotegerin: receptor activator of nuclear factor k-β ligand (OPG:RANKL) ratio at 21-days for Si-TCP and Zn-TCP scaffolds. These results demonstrate the effects of Si and Zn doped porous β-TCP scaffolds on the upregulation of osteoblast marker gene expression including OPG, RANKL, BMP-2, and Runx2, indicating the role of trace elements on the effective regulation of late-stage osteoblast cell differentiation markers.

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“…The decrease in the vertical axis is caused by the fact that the amount of N 2 absorbed by the composite reduces by decreasing pressure. A standard BET multi-point was used to calculate the HA structure modification usually affects the adsorption-desorption behavior, surface area, total pore volume, and average pore size [28][29][30]. Table 2 shows that HASiO 2 and HASiO 2 _PE have a specific surface area of 59.295 and 43.350 m²/g, respectively.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…The combined mapping showed that the composite surface is uneven and that the PE (arrows) agglomerates on some of the surface's area. Such a surface supports the attachment of cells and ions when the composite is in a physiological environment [29].…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Calcium Phosphate Cement Composed of Hydroxyapatite Modified Silica and Polyeugenol as a Bone Filler Material

et al. 2023

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A composite of hydroxyapatite modified silica (HASiO2) and 10% (w/w) polyeugenol (PE) was synthesized to produce a calcium phosphate cement with antibacterial activity. The compatibility of the composite (HASiO2_PE) with bone filler requirements was determined due to its crystal, surface, antibacterial, and cytocompatibility properties. The results showed that compositing HASiO2 and PE did not affect HA's chemical dan crystal properties. The presence of PE changed HASiO2 morphology to be coarser and denser than before composited. PE tends to agglomerate but does not affect the hydrophilicity of HASiO2. The presence of PE increased the surface area and total pore volume but lowered the average pore size. Different from pure PE, the composite of HASiO2_PE that contains of 10% PE has higher antibacterial activity toward Escherichia coli than Staphylococcus aureus. The composite is biocompatible because the cytotoxicity test toward pre-osteoblast cells resulted in an IC50 of 2092 μg/mL. Thus, due to its chemical, surface, antibacterial, and cytocompatibility properties, the HASiO2_PE composite can be recommended as a bone filler material.

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Preparation of micro-porous bioceramic containing silicon-substituted hydroxyapatite and beta-tricalcium phosphate

Cited by 36 publications

References 47 publications

Synthesis and characterizations of natural limestone-derived nano-hydroxyapatite (HAp): a comparison study of different metals doped HAps on antibacterial activity

Synthesis and characterizations of natural limestone-derived nano-hydroxyapatite (HAp): a comparison study of different metals doped HAps on antibacterial activity

Regulation of Osteogenic Markers at Late Stage of Osteoblast Differentiation in Silicon and Zinc Doped Porous TCP

Calcium Phosphate Cement Composed of Hydroxyapatite Modified Silica and Polyeugenol as a Bone Filler Material

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