Fast axial-scanning photoacoustic microscopy using tunable acoustic gradient lens

Yang, Xiaoquan; Jiang, Bowen; Song, Xianlin; Wei, Jianshuang; Luo, Qingming

doi:10.1364/oe.25.007349

Cited by 38 publications

(19 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This can be implemented using the geometry of dual-beam illumination on a fast-scanning imaging head or by applying a tunable acoustic gradient lens. 32 Moreover, the degradation of lateral resolution due to optical scattering may be partially mitigated within a certain depth range by implementing PAM with adaptive optics. 33 DAI-PAM could also benefit quantitative multiwavelength PAM for measuring blood oxygenation in the cortex, such as the oxygen saturation in cerebral diving vessels demonstrated in this study.…”

Section: Discussionmentioning

confidence: 99%

Dual-axis illumination for virtually augmenting the detection view of optical-resolution photoacoustic microscopy

Hsu

Yao

et al. 2018

J. Biomed. Opt.

View full text Add to dashboard Cite

Abstract. Optical-resolution photoacoustic microscopy (OR-PAM) has demonstrated fast, label-free volumetric imaging of optical-absorption contrast within the quasiballistic regime of photon scattering. However, the limited numerical aperture of the ultrasonic transducer restricts the detectability of the photoacoustic waves, thus resulting in incomplete reconstructed features. To tackle the limited-view problem, we added an oblique illumination beam to the original coaxial optical-acoustic scheme to provide a complementary detection view. The virtual augmentation of the detection view was validated through numerical simulations and tissue-phantom experiments. More importantly, the combination of top and oblique illumination successfully imaged a mouse brain in vivo down to 1 mm in depth, showing detailed brain vasculature. Of special note, it clearly revealed the diving vessels that were long missing in images from original OR-PAM.

show abstract

Section: Discussionmentioning

confidence: 99%

Dual-axis illumination for virtually augmenting the detection view of optical-resolution photoacoustic microscopy

Hsu

Yao

et al. 2018

J. Biomed. Opt.

View full text Add to dashboard Cite

show abstract

“…When tumor volume of H22 tumor-bearing mice grew up to 100-150 mm 3 , the mice were intratumorally injected with AuNCs or CAuNCs at Au dose of 10 mg/kg. At different time intervals, the mice were scanned on a homemade photoacoustic system to collect the photoacoustic signals 37 , 38 . The wavelength of laser was 744 nm and the laser energy per pulse was 200 nJ.…”

Section: Methodsmentioning

confidence: 99%

A Biomimetic Gold Nanocages-Based Nanoplatform for Efficient Tumor Ablation and Reduced Inflammation

Wan

Bie

et al. 2018

Theranostics

Self Cite

View full text Add to dashboard Cite

Gold nanocages (AuNCs), with high photothermal conversion efficiency and unique hollow interiors, have become a promising nanoplatform for synergistic phototheraml therapy (PTT)-chemotherapy. However, the insufficient tumor targeting, in vivo premature drug leakage and low drug loading efficiency responsible for the spatial-temporal un-synchronization of PTT-chemotherapy, as well as inflammatory response might compromise the anticancer treatment of AuNCs-based drug delivery systems.Methods: Cancer cell membrane (CCM)-coated AuNCs were developed to load anticancer drug doxorubicin (DOX@CAuNCs) by transmembrane ammonium sulfate gradient method. In vitro and in vivo analysis, including characterization, macrophage phagocytosis and tumor targeting capacity, near-infrared (NIR) laser-induced drug release, antitumor efficacy and inflammation response were systematically performed.Results: DOX@CAuNCs showed a high DOX loading capacity and on-demand NIR laser-triggered DOX release compared with CAuNCs passively loading DOX by electrostatic adsorption, a commonly used method to load drug to AuNCs. Meanwhile, in view of the properties of CCM coated on AuNCs, DOX@CAuNCs exhibited decreased macrophage phagocytosis, prolonged blood circulation and enhanced internalization by cancer cells, generating preferable tumor targeting ability. With these integrated advantages, DOX@CAuNCs demonstrated highly efficient and precise spatial-temporal synchronization of PTT-chemotherapy, achieving complete tumor ablation with no obvious side effects. Besides, coating with CCM significantly alleviated AuNCs-induced inflammatory response.Conclusion: This biomimetic AuNCs-based platform might be a prospective drug delivery system for precision PTT and chemotherapy, acquiring desired cancer treatment efficacy and low inflammatory response.

show abstract

“…The TAG lens is an adaptive lens that can change its effective focal length in a few microseconds or less [64], so it has been widely used for varifocal imaging [[65], [66], [67]]. Yang et al in 2017 reported an OR-PAM system using a TAG lens to extend the DoF [68]. The TAG lens consisted of a cylindrical piezoelectric shell filled with silicone oil and its focal length can be shifted according to the applied sinusoidal signal.…”

Section: Image Enhancement In Out-of-focus Regionsmentioning

confidence: 99%

Review on practical photoacoustic microscopy

et al. 2019

View full text Add to dashboard Cite

Photoacoustic imaging (PAI) has many interesting advantages, such as deep imaging depth, high image resolution, and high contrast to intrinsic and extrinsic chromophores, enabling morphological, functional, and molecular imaging of living subjects. Photoacoustic microscopy (PAM) is one form of the PAI inheriting its characteristics and is useful in both preclinical and clinical research. Over the years, PAM systems have been evolved in several forms and each form has its relative advantages and disadvantages. Thus, to maximize the benefits of PAM for a specific application, it is important to configure the PAM system optimally by targeting a specific application. In this review, we provide practical methods for implementing a PAM system to improve the resolution, signal-to-noise ratio (SNR), and imaging speed. In addition, we review the preclinical and the clinical applications of PAM and discuss the current challenges and the scope for future developments.

show abstract

Fast axial-scanning photoacoustic microscopy using tunable acoustic gradient lens

Cited by 38 publications

References 18 publications

Dual-axis illumination for virtually augmenting the detection view of optical-resolution photoacoustic microscopy

Dual-axis illumination for virtually augmenting the detection view of optical-resolution photoacoustic microscopy

A Biomimetic Gold Nanocages-Based Nanoplatform for Efficient Tumor Ablation and Reduced Inflammation

Review on practical photoacoustic microscopy

Contact Info

Product

Resources

About