Dual-axis illumination for virtually augmenting the detection view of optical-resolution photoacoustic microscopy

Hsu, Hsun‐Chia; Li, Lei; Yao, Junjie; Wong, Terence T. W.; Shi, Junhui; Chen, Ruimin; Zhou, Qifa; Wang, Lihong V.

doi:10.1117/1.jbo.23.7.076001

Cited by 7 publications

(2 citation statements)

References 34 publications

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“…Simultaneous recordings of genetically encoded fluorescent calcium indicators and PAI of hemodynamic parameters could shed light on individual contributions of neurons and glial cells to CBF increases (Muñoz et al, 2015). To achieve these results, OR-PAM techniques usually still necessitate scalp and sometimes also skull removal and are limited to a penetration depth of about a millimeter (Hsu et al, 2018).…”

Section: Imaging Of Brain Hemodynamicsmentioning

confidence: 99%

Photoacoustic Neuroimaging - Perspectives on a Maturing Imaging Technique and its Applications in Neuroscience

Bodea

Westmeyer

2021

Front. Neurosci.

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A prominent goal of neuroscience is to improve our understanding of how brain structure and activity interact to produce perception, emotion, behavior, and cognition. The brain’s network activity is inherently organized in distinct spatiotemporal patterns that span scales from nanometer-sized synapses to meter-long nerve fibers and millisecond intervals between electrical signals to decades of memory storage. There is currently no single imaging method that alone can provide all the relevant information, but intelligent combinations of complementary techniques can be effective. Here, we thus present the latest advances in biomedical and biological engineering on photoacoustic neuroimaging in the context of complementary imaging techniques. A particular focus is placed on recent advances in whole-brain photoacoustic imaging in rodent models and its influential role in bridging the gap between fluorescence microscopy and more non-invasive techniques such as magnetic resonance imaging (MRI). We consider current strategies to address persistent challenges, particularly in developing molecular contrast agents, and conclude with an overview of potential future directions for photoacoustic neuroimaging to provide deeper insights into healthy and pathological brain processes.

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Section: Imaging Of Brain Hemodynamicsmentioning

confidence: 99%

Photoacoustic Neuroimaging - Perspectives on a Maturing Imaging Technique and its Applications in Neuroscience

Bodea

Westmeyer

2021

Front. Neurosci.

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“…Moreover, fPAM still cannot image all of the cerebral vessels due to insufficient resolution and the limited-view issue. 41 Innovation on the optical illumination and ultrasonic detection designs to address this limitation is also an important future exploration.…”

Section: Temperal Profile Of the Initial Dipmentioning

confidence: 99%

Wave of single-impulse-stimulated fast initial dip in single vessels of mouse brains imaged by high-speed functional photoacoustic microscopy

Shi

Maslov

et al. 2020

J. Biomed. Opt.

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Significance: The initial dip in hemoglobin-oxygenation response to stimulations is a spatially confined endogenous indicator that is faster than the blood flow response, making it a desired label-free contrast to map the neural activity. A fundamental question is whether a single-impulse stimulus, much shorter than the response delay, could produce an observable initial dip without repeated stimulation. Aim: To answer this question, we report high-speed functional photoacoustic (PA) microscopy to investigate the initial dip in mouse brains. Approach: We developed a Raman-laser-based dual-wavelength functional PA microscope that can image capillary-level blood oxygenation at a 1-MHz one-dimensional imaging rate. This technology was applied to monitor the hemodynamics of mouse cerebral vasculature after applying an impulse stimulus to the forepaw. Results: We observed a transient initial dip in cerebral microvessels starting as early as 0.13 s after the onset of the stimulus. The initial dip and the subsequent overshoot manifested a wave pattern propagating across different microvascular compartments. Conclusions: We quantified both spatially and temporally the single-impulse-stimulated microvascular hemodynamics in mouse brains at single-vessel resolution. Fast label-free imaging of single-impulse response holds promise for real-time brain-computer interfaces.

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Detection of weak optical absorption by optical-resolution photoacoustic microscopy

et al. 2022

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Dual-axis illumination for virtually augmenting the detection view of optical-resolution photoacoustic microscopy

Cited by 7 publications

References 34 publications

Photoacoustic Neuroimaging - Perspectives on a Maturing Imaging Technique and its Applications in Neuroscience

Photoacoustic Neuroimaging - Perspectives on a Maturing Imaging Technique and its Applications in Neuroscience

Wave of single-impulse-stimulated fast initial dip in single vessels of mouse brains imaged by high-speed functional photoacoustic microscopy

Detection of weak optical absorption by optical-resolution photoacoustic microscopy

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