Protective effect of alpha-lipoic acid in methotrexate-induced ovarian oxidative injury and decreased ovarian reserve in rats

Karapınar, Oya Soylu; Pınar, Neslihan; Özcan, Oğuzhan; Özgür, Tümay; Dolapçıoğlu, Kenan

doi:10.1080/09513590.2017.1306847

Cited by 25 publications

(15 citation statements)

References 33 publications

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“…The etiology and pathogenesis of DOR remain unclear so far, but it has been reported that DOR may be related to age (Fan et al, 2019), immunity (Silva et al, 2014), heredity, environment, radiotherapy and chemotherapy (Smith et al, 2013), surgery, psychological stress (Nicoloro-SantaBarbara et al, 2017), and infectious factors (Soylu Karapinar et al, 2017). A prospective cohort study found that poor ovarian response was related to age and apoptosis of ovarian granulosa cells (Fan et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Protective Effects and Possible Mechanisms of Actions of Bushen Cuyun Recipe on Diminished Ovarian Reserve Induced by Cyclophosphamide in Rats

et al. 2020

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Backgrounds: Diminished ovarian reserve (DOR) contributes significantly to female infertility. Bushen Cuyun Recipe (BCR, Tradename Yueliang Yin), a product marketed in China, has shown effects in the treatment of female infertility in clinical practices of traditional Chinese medicine (TCM). In this study, we aimed to investigate the chemical compositions of BCR and its efficacy based on scientific evidence and pharmacological mechanisms in DOR treatments. Methods: The chemical compositions of BCR were determined by the UHPLC-LTQ-Orbitrap MS method. DOR was induced in a rat model by intraperitoneal injection of cyclophosphamide (CTX) 90 mg/kg once. After the CTX treatment for 14 days, rats were intragastrically administrated deionized water, dehydroepiandrosterone (DHEA), or BCR in low, middle, and high doses for 30 days. Ovarian index, ovarian morphology, follicle number, and anti-Müllerian hormone (AMH) in serum were determined to assess the effects of BCR. To investigate possible action mechanisms, network pharmacological analysis was used to predict possible pathways in the effects of BCR on female infertility. In experimental studies, the contents of hormones in the hypothalamic-pituitary-ovarian axis (HPOA, including estradiol (E 2), follicle-stimulating hormone (FSH), and gonadotropinreleasing hormone (GnRH)) and pyroptosis-related proteins, including gasdermin D (GSDMD), caspase-1, and interleukin-18 (IL-18), in ovarian were detected by ELISA, immunofluorescence and Western blot. Results: Chemical studies revealed a total 84 components in BCR, which included 43 flavonoids, 13 triterpenoids, 11 phenolic acids, 8 alkaloids, 1 coumarin, 1 anthraquinone, and 7 other components. After treatments with BCR, the ovarian morphology, ovarian index, estrous cycle, growing follicles and corpus luteum from last ovulation, and serum AMH in DOR rats were significantly improved. Network pharmacological analysis suggested that the NOD-like receptor signaling pathway ranked No. 1 among the mechanisms by which BCR affects female infertility. Experimental results demonstrated

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Section: Introductionmentioning

confidence: 99%

Protective Effects and Possible Mechanisms of Actions of Bushen Cuyun Recipe on Diminished Ovarian Reserve Induced by Cyclophosphamide in Rats

et al. 2020

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“…Similar to ours, in a study where instead of DHEA, alpha-lipoic acid utilized; it reported that in rats that received MTX, alpha-lipoic acid has reversed the AMH levels and the total follicle counts, and thus healing the oxidative damage to the ovaries. Karapınar and colleagues have concluded that the oxidative effects of MTX on the ovaries may be inhibited by alpha-lipoic acid supplementation which is an antioxidant (Soylu Karapinar, Pinar, Özcan, Özgür, & Dolapçıoğlu, 2017).…”

Section: Resultsmentioning

confidence: 99%

The Effect of Dehydroepiandrosterone on Ovarian Reserve in Ovarian Damage Caused by Methotrexate

et al. 2020

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To determine the possible detrimental effects of multiple methotrexate doses has on the ovarian reserve and to determine the beneficial effects of dehydroepiandrosterone supplementation. The rats (n:24) divided into three groups; Group 1: control group, Group 2: dehydroepiandrosterone and methotrexate group (6mg/kg dehydroepiandrosterone dissolved in 0.1 ml sesame seed oil subcutaneously for ten days and 1mg/kg intramuscular methotrexate at the 1st, 3rd, 5th and 7th days) and Group 3: methotrexate group (1mg/kg intramuscular methotrexate at the 1st, 3rd, 5th and 7th day). The groups compared in regards to their histopathological ovarian damage scores and AMH values. It established that multiple methotrexate applications had a considerable effect on reducing vascular congestion in the ovarian tissue. Both in groups 2 and 3 AMH values found to be significantly lower. When this decline in the ovarian reserve examined comparatively; while both the group 2 and 3 reported having a considerable and continuous reduction in the AMH levels correlative to the control group; the primordial, primary and total follicle counts shown to stay statistically the same in the group 2 (p<0.0167). The dehydroepiandrosterone supplementation, while did not have a significant effect on reducing ovarian damage induced by methotrexate, also was not shown to provide any considerable increase in AMH levels. However, prolonged usage of dehydroepiandrosterone by contributing to the antral follicle pool considered promising in increasing the AMH levels conceivably

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“…The oxidative stress is deemed to be one of the dominating mechanisms of ovarian failure (Devine et al., 2012; Luderer, 2014). It has been reported that intragastric or intraperitoneal administration of ALA improves oxidative damage to the ovaries (Avci, Bahadir, Bahadir, Tuncel, & Bilgici, 2016; Soylu Karapinar, Pinar, Pinar, Ozcan, Ozgur, & Dolapcioglu, 2017). In this study, 600 mg/kg ALA‐treated group decreased the level of MDA and improved the activity of TAOC and SOD in ovary.…”

Section: Discussionmentioning

confidence: 99%

Alpha‐lipoic acid improves the reproduction performance of breeder hens during the late egg‐laying period

Dai

et al. 2020

Animal Physiology Nutrition

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Breeder hens in the late egg-laying period have lower reproductive performance, manifesting as degraded egg-laying rate, quality and hatching performance. The production performance of laying hens is influenced by breeder hens (Gong et al., 2019; Lang et al., 2019). The Qiling hen, used in the present study, is a Chinese native chicken. It is worth mentioning that, the reproductive performance of these local hens decreases rapidly in the late egg-laying period. Therefore, it is of great significance to find a solution to this problem from the perspective of nutritional regulation. In poultry, many factors are in connection with fertility.

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Protective effect of alpha-lipoic acid in methotrexate-induced ovarian oxidative injury and decreased ovarian reserve in rats

Cited by 25 publications

References 33 publications

Protective Effects and Possible Mechanisms of Actions of Bushen Cuyun Recipe on Diminished Ovarian Reserve Induced by Cyclophosphamide in Rats

Protective Effects and Possible Mechanisms of Actions of Bushen Cuyun Recipe on Diminished Ovarian Reserve Induced by Cyclophosphamide in Rats

The Effect of Dehydroepiandrosterone on Ovarian Reserve in Ovarian Damage Caused by Methotrexate

Alpha‐lipoic acid improves the reproduction performance of breeder hens during the late egg‐laying period

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