Prevalence of plasmid-mediated quinolone resistance in &lt;i&gt;Escherichia coli&lt;/i&gt; isolated from diseased animals in Taiwan

Yeh, Jih-Ching; Lo, Dan-Yuan; Chang, Su-Wei; Chou, Chi‐Chung; Kuo, Hung-Chih

doi:10.1292/jvms.16-0463

Cited by 16 publications

(9 citation statements)

References 30 publications

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“…In this study, 11 FQ-resistant APEC isolates carried 2 types of PMQR genes, qnrS (3 isolates) and qnrA (8 isolates). This result is consistent with recent studies of APEC isolates from Egypt, Taiwan, and South Korea ( Ahmed et al., 2013 , Yeh et al., 2017 , Seo and Lee, 2019 ). The PMQR genes may contribute to the increased prevalence of resistant mutants by conferring a low resistance level in a population ( Varela et al., 2015 ).…”

Section: Discussionsupporting

confidence: 93%

Molecular characteristics of fluoroquinolone-resistant avian pathogenic Escherichia coli isolated from broiler chickens

Yoon¹,

Kim

Ha³

et al. 2020

Poultry Science

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Avian pathogenic Escherichia coli ( APEC ) is a major pathogen in the poultry industry worldwide including Korea. In this study, the phenotypic and genotypic characteristics of 33 fluoroquinolone ( FQ )-resistant APEC isolates from broilers were analyzed. All FQ-resistant APEC isolates showed amino acid exchanges at both gyrA and parC and high minimal inhibitory concentrations for FQs. A total of 11 (33.3%) isolates were positive for the plasmid-mediated quinolone resistance ( PMQR ) genes, qnrA (8 isolates) and qnrS (3 isolates), and showed multidrug resistance. Among the 11 PMQR-positive isolates, 1 and 2 isolates carried bla CTX-1 and bla CTX-15 , respectively, as extended-spectrum β-lactamase ( ESBL ) producers, and the non-ESBL gene, bla TEM-1 , was found in 4 isolates. Among 3 aminoglycoside-resistant isolates, aac(3)-II was only detected in 1 isolate. All 8 APEC isolates with resistance to tetracycline carried the tetA gene. Overall, 6 of the 7 trimethoprim-sulfamethoxazole-resistant isolates carried the sul1 or sul2 genes, while only 2 of the 8 chloramphenicol-resistant isolates carried the catA1 gene. Although 9 isolates carried class I integrons, only 4 isolates carried the gene cassettes dfr A12- aad A2 (2 isolates), dfr A17- aa dA5 (1 isolate), ext X- psp-aad A2 (1 isolate), and dfrA27 (1 isolate). The most common plasmid replicon was FIB (8 isolates, 72.7%), followed by K/B (4 isolates, 36.4%). Antimicrobial resistance monitoring and molecular analysis of APEC should be performed continuously to surveil the transmission between poultry farms.

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Section: Discussionsupporting

confidence: 93%

Molecular characteristics of fluoroquinolone-resistant avian pathogenic Escherichia coli isolated from broiler chickens

Yoon¹,

Kim

Ha³

et al. 2020

Poultry Science

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“…Yeh et al . [23] reported 50.6% ENR resistance and 42.3% CIP resistance of E. coli isolates from poultry in Taiwan. The resistance of quinolone in European countries in poultry was about 52% [7].…”

Section: Discussionmentioning

confidence: 99%

Molecular detection of genes encoding resistance to tetracycline and determination of plasmid-mediated resistance to quinolones in avian pathogenic Escherichia coli in Sukabumi, Indonesia

et al. 2018

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Aim:This study aimed to identify genes encoding resistance to tetracycline (TE) and plasmid-mediated resistance to quinolones in Escherichia coli isolates from clinical cases of avian colibacillosis in Sukabumi, Indonesia.Materials and Methods:A total of 25 E. coli archive isolates were collected in 2013-2017 from clinical cases of avian colibacillosis in Sukabumi, Indonesia. All isolates were tested for TE and quinolone resistance using the disk diffusion method. TE -resistant E. coli isolates were screened for the presence of tet(A) and tet(B) genes by single polymerase chain reaction (PCR). The qnr(A), qnr(B), and qnr(S) genes were detected by multiplex PCR in quinolone-resistant E. coli isolates.Results:Result of this study shows that 19 of 25 (76%) E. coli isolates are resistant to oxytetracycline and 64% are resistant to TE; among them, 63.2% and 31.5% were positive tet(A) and tet(B), respectively. 13 out of 25 (52%) are resistant to ciprofloxacin and 36% are resistant to enrofloxacin either norfloxacin; among them, 61.6% were positive qnr(A), 7.7% were positive qnr(B), 23% were positive qnr(S), and 7.7% were positive both of qnr(A) and qnr(S).Conclusion:This study shows that a few pathogens of E. coli are resistant to TE and quinolone. The frequency of tet and qnr genes that are responsible for this resistance among avian pathogenic E. coli isolates in Sukabumi, Indonesia, was high.

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“…Fluroquinolones (FQs) such as ciprofloxacin are widely used in the therapy of typhoid fever because of their high efficacy, lesser side effects and convenient oral dosages [6,7]. However, the extensive use of these agents has led to the development of bacterial resistance to quinolones over time [6,8,9] Fluoroquinolone resistance in now gram-negative bacteria is infact a global issue [10] and is known to be mediated in S. typhi by point mutations in quinolone resistance determining regions (QRDR) of the genes encoding DNA gyrase (gyrA and gyrB) and topoisomerase IV (parC and parE); and carriage of the plasmidmediated quinolone resistance (PMQR) genes [7,11,48]. Plasmid-mediated quinolone resistance was first reported in 1998 in the United States in a multiresistant urinary Klebsiella pneumoniae; but it is now being reported in clinical and environmental isolates of other gram-negative as well as and gram-positive bacteria, and appears to be spreading [11,12].…”

Section: Introductionmentioning

confidence: 99%

Plasmid-mediated Quinolone Resistance Genes in Salmonella typhi from Patients Attending Selected General Hospitals in Abuja Municipal, Nigeria

Fasema

Bassey

Ngwai

et al. 2020

AJBGMB

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This study investigated the antimicrobial resistance profile and presence of plasmid-mediated quinolone resistance (PMQR) genes in Salmonella typhi from patients attending selected general hospitals in Abuja municipal, Nigeria. Four hundred stool samples from patients with suspected typhoid fever were collected from Asokoro General Hospital Abuja (AGH), Garki Hospital Abuja (GHA), Maitama General Hospital Abuja (MGHA) and Wuse General Hospital Abuja (WGHA) and S. typhi was isolated and identified using standard microbiological methods. Antimicrobial susceptibility testing of the isolates was carried out using Clinical and Laboratory Standards Institute (CLSI) method. Molecular detection of PMQR genes in the ciprofloxacin-resistant isolates was carried out using Polymerase Chain Reaction (PCR) method. The overall occurrence of the isolates was 13.3% (53/400), with the highest hospital-related occurrence at WGHA (18.0%). The occurrence was highest at age 21-30yrs in AGHA (20.0%), GHA (33.3%) and WGHA (45.0%). The occurrence was higher in females at AGHA (12.7%) and GHA (16.0%); but higher in males at MGHA (11.4%) and WGHA (18.2%). Resistance to ciprofloxacin was the least at 30.2%, distributed as follows: AGHA (20.0%), GHA (35.7%), MGHA (36.4%) and WGHA (27.8%). The most common resistance phenotype was: NA-S-XT-AMC-TE-CRO-C-CN with overall occurrence of 9.4% (5/53) observed in AGH (10.0%), GHA (16.7%) and MGHA (18.2%) but not in WGHA. All (100%) isolates were multiple antibiotic resistance (MAR) isolates, with MAR indices above 0.2; and the commonest MAR index of 0.6 (30.0%) in AGHA, 0.8 (35.7%) in GHA; 0.8 (45.6%) in MGHA, and was 0.7 (38.9%) in WGHA. Multidrug resistance (MDR) was the commonest at 96.2% (51/53), with occurrences in the selected hospitals as follows: AGHA (90.0%), GHA (100.0%) and MGHA (100.0%) and WGHA (94.4%).The PMQR genes detected had overall frequency in the order: aac(6′)-Ib-cr (50.0%) >qnrB (37.5%) >qnrS (18.8%); qnrS was absent in AGHA and WGHA. The genes co-existed with one another with the qnrB+ aac(6′)-Ib-cr combination, present in isolates from all the hospitals, being the most common at (31.3%). Ciprofloxacin was the most effective antibiotic against the isolates; most isolates were MAR with prior exposure to antibiotics; most isolates were MDR and the ciprofloxacin-resistant isolatesharbored qnrS, qnrB and aac(6′)-Ib-cr PMQR genes, with aac(6′)-Ib-cr being the most prevalent.

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Prevalence of plasmid-mediated quinolone resistance in <i>Escherichia coli</i> isolated from diseased animals in Taiwan

Cited by 16 publications

References 30 publications

Molecular characteristics of fluoroquinolone-resistant avian pathogenic Escherichia coli isolated from broiler chickens

Molecular characteristics of fluoroquinolone-resistant avian pathogenic Escherichia coli isolated from broiler chickens

Molecular detection of genes encoding resistance to tetracycline and determination of plasmid-mediated resistance to quinolones in avian pathogenic Escherichia coli in Sukabumi, Indonesia

Plasmid-mediated Quinolone Resistance Genes in Salmonella typhi from Patients Attending Selected General Hospitals in Abuja Municipal, Nigeria

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