Purification, characterization and procoagulant activity of polysaccharides from Angelica dahurice roots

Wang, Jinmei; Lian, Pengli; Yu, Qi; Wei, Jinfeng; Kang, Wenyi

doi:10.1186/s13065-017-0243-y

Cited by 26 publications

(10 citation statements)

References 1 publication

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“…First, the derivatives (hydrolysis and acetylation) of PCp-I and PCp-II were carried out, and monosaccharide compositions were then analyzed by Gas Chromatography (GC, GC-2010, Shimadzu, Japan) equipped with a HP capillary column (30 m × 0.35 mm × 0.25 μm, Aglient Technologies, Inc., PaloAlto, Santa Clara, CA, USA) and a FID detector using the published method [49]. The standard monosaccharides (glucose, xylose, arabinose, rhamnose, mannose, ribose, fructose, and galactose) were derivatized and subjected to GC analysis in the same way.…”

Section: Methodsmentioning

confidence: 99%

Two Novel Polysaccharides in Psoralea corylifolia L and anti-A549 Lung Cancer Cells Activity In Vitro

Yin

Zhang

et al. 2019

Molecules

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Two novel water soluble heteroglycan (PCp-I and PCp-II) with anti-A549 lung cancer cells activity were isolated from Psoralea corylifolia L. Their average molecular weights were 2.721 × 104 and 2.850 × 104. PCp-I and PCp-II had the same monosaccharide composition, but their molar ratios were different. Based on methylation and NMR spectroscopy, the part structure of PCp-I was identified. The results of scanning electron microscope (SEM) showed that PCp-I had an irregular porous structure and PCp-II was flaky and irregularly curved. The results of thermogravimetry-differential scanning calorimetry (TG-DSC) showed that PCp-I and PCp-II had good thermal stability. Furthermore, PCp-I and PCp-II exhibited significant anti-A549 lung cancer cells activity (IC50 = 64.84 and 126.30 μM) in vitro.

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Section: Methodsmentioning

confidence: 99%

Two Novel Polysaccharides in Psoralea corylifolia L and anti-A549 Lung Cancer Cells Activity In Vitro

Yin

Zhang

et al. 2019

Molecules

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“…In our previous study, we found that several polysaccharides have coagulation or anticoagulation activities [9][10][11][12]. Up to date, there is no research on the coagulation effect of the F. velutipes polysaccharides.…”

Section: Introductionmentioning

confidence: 97%

Structural Identification and Coagulation Effect of Flammulina velutipes Polysaccharides

et al. 2021

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Two polysaccharides were isolated successfully from Flammulina velutipes and identified as CHFVP-1 (24.44 kDa) and CHFVP-2 (1497 kDa). Based on the results of Fourier transform-infrared spectroscopy (FT-IR), gas chromatography (GC), gas chromatography–mass spectrometry (GC–MS), and nuclear magnetic resonance (NMR) spectroscopy regarding the structure of CHFVP-1 and CHFVP-2, CHFVP-1 was constructed with the backbone of→6)-α-D-Galp-(1→ and the branch of Galp by an →3,6)-α-D-Manp-(1→attached with T-β-D-Glcp or t-α-L-Fucp side chains. Meanwhile, the CHFVP-2 was a glucan with the construction of →6)-β-D-Glcp-(1→ and T-β-D-Glcp. Moreover, the coagulant activity in vitro of CHFVP-1 and CHFVP-2 was evaluated, and the results showed that CHFVP-1 exerts procoagulant activity by shortening the activated partial thromboplastin time (APTT) and thrombin time (TT), while CHFVP-2 did not reveal a definite coagulant activity. The finding would benefit the further application of F. velutipes in the field of medicine.

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“…The four polysaccharide fractions were further purified by Toyopearl-H65F gel resin (1.6 × 80 cm) and eluted with 0.2 mol/l NaCl at a flow rate of 0.5 ml/min [16, 17]. Then the solution was collected in accordance with four elution peaks (DOP-40, DOP-50, DOP-60, and DOP-70), concentrated, and dialyzed against distilled water for 48 h, fresh distilled water was replaced every 4 h, respectively.…”

Section: Methodsmentioning

confidence: 99%

Physicochemical properties of polysaccharides from Dendrobium officinale by fractional precipitation and their preliminary antioxidant and anti-HepG2 cells activities in vitro

Xing

Zhang

et al. 2018

Chemistry Central Journal

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BackgroundDendrobium officinale as a precious traditional Chinese herb is widely used in medicines and health supplements. Thus the extraction, purification and biological activities of polysaccharides from the stem of Dendrobium officinale have significant meaning on theory and application value.MethodsThe crude Dendrobium officinale polysaccharide (DOP) was obtained by hot water extraction- ethanol precipitation method, and four new polysaccharide fractions (DOP-40, DOP-50, DOP-60, and DOP-70) were further obtained from the crude DOP by fractional precipitation with ethanol method, then four fractions were further purified by Toyopearl-H65F gel resin. The molecular weight and monosaccharide composition of four purified fractions were determined by high performance anion exchange chromatography and high performance liquid chromatography. The antioxidant activities of them were evaluated by the reducing power assay, and the superoxide anion, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and hydroxyl free radicals scavenging assays, respectively. Finally, the anticancer activities of them were investigated via the MTT assay and the western blot analysis using HepG2 cells.ResultsAmong these four purified fractions were mainly composed of d-mannose and d-glucose with different molar ratios, and their average molecular weights were 999, 657, 243 and 50.3 kDa, respectively. What’s more, DOP-70 always exhibited the strongest antioxidant and anticancer activities, while DOP-40 and DOP-60 showed very close antioxidant and anticancer activities which were better than that of DOP-50. The western blotting analysis also showed that DOP-40, DOP-60, and DOP-70 induced apoptosis in HepG2 human liver cancer cells through the Bcl-2 and Bax-dependent pathway.ConclusionsFractional precipitation with ethanol could successfully apply to extract four new polysaccharide fractions from Dendrobium officinale stems, and the polysaccharide fractions possessed efficient antioxidant and anticancer activities, especially DOP-70.

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Purification, characterization and procoagulant activity of polysaccharides from Angelica dahurice roots

Cited by 26 publications

References 1 publication

Two Novel Polysaccharides in Psoralea corylifolia L and anti-A549 Lung Cancer Cells Activity In Vitro

Two Novel Polysaccharides in Psoralea corylifolia L and anti-A549 Lung Cancer Cells Activity In Vitro

Structural Identification and Coagulation Effect of Flammulina velutipes Polysaccharides

Physicochemical properties of polysaccharides from Dendrobium officinale by fractional precipitation and their preliminary antioxidant and anti-HepG2 cells activities in vitro

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