Two Novel Polysaccharides in Psoralea corylifolia L and anti-A549 Lung Cancer Cells Activity In Vitro

Abstract

Two novel water soluble heteroglycan (PCp-I and PCp-II) with anti-A549 lung cancer cells activity were isolated from Psoralea corylifolia L. Their average molecular weights were 2.721 × 104 and 2.850 × 104. PCp-I and PCp-II had the same monosaccharide composition, but their molar ratios were different. Based on methylation and NMR spectroscopy, the part structure of PCp-I was identified. The results of scanning electron microscope (SEM) showed that PCp-I had an irregular porous structure and PCp-II was flaky and irregularly curved. The results of thermogravimetry-differential scanning calorimetry (TG-DSC) showed that PCp-I and PCp-II had good thermal stability. Furthermore, PCp-I and PCp-II exhibited significant anti-A549 lung cancer cells activity (IC50 = 64.84 and 126.30 μM) in vitro.

Pharmacokinetic study on the interaction between pachymic acid and bavachin and its potential mechanism

“… 2015 ; Yin et al. 2019 ). Combination therapy of different drugs might induce adverse interaction that leads to treatment failure and even toxicity (Hu et al.…”

“…Co-administration of various drugs possesses multi-target and multi-level function characteristics, especially in intractable diseases with complications (Huang et al 2018). Pachymic acid and bavachin are major active ingredients of P. cocos and P. corylifolia, respectively, which are commonly combined in the therapeutic prescription of lung cancer due to their similar anti-tumor effect (Ling et al 2010;Ma et al 2015;Yin et al 2019). Combination therapy of different drugs might induce adverse interaction that leads to treatment failure and even toxicity (Hu et al 2005).…”

“…In the therapy of lung cancer, Psoralea corylifolia Linn. (Fabaceae) is also a commonly used herbal medicine, which can inhibit cell activity of lung cancer (Yin et al 2019). Bavachin is the main flavonoid in P. corylifolia that has been reported to have antibacterial, antiinflammation, and antidiabetic activities (Yin et al 2004;Alam et al 2018;Hung et al 2019).…”

Activation of RAW264.7 cells by PCp-I, a polysaccharide from Psoralea corylifolia L, through NF-κB/MAPK signalling pathway

“…The PCp-I was composed of rhamnose, arabinose, xylose, mannose, glucose and galactose. 2 Polysaccharide has a wide range of biological activities, such as immunomodulation, [3][4][5] anti-tumor, 6 anti-oxidation 7,8 and anti-virus. 9 Immunomodulatory activity is recognized as the most important effective biological effects of polysaccharide and some scholars have found that the structure of polysaccharide including the monosaccharide composition, glycosidic-linkage position, molecular weight and branching are closely Activation of RAW264.7 cells by PCp-I, a polysaccharide from Psoralea corylifolia L, through NF-κB/MAPK signalling pathway related to its immunostimulatory activity.…”

“…11 Inhibiting effect of PCp-I on the A549 lung cancer cells was confirmed in our previous research while the immune activity has not been studied. 2 The main targets responding to immune molecules are monocytes, macrophages and neutrophils. 11 In this research, the mouse macrophage RAW264.7 was selected to study the immunomodulatory effects of PCp-I.…”