Protection of Penaeus monodon against white spot syndrome by continuous oral administration of a low concentration of Bacillus subtilis spores expressing the VP28 antigen

Abstract

This study reports the protective efficacy of Bacillus subtilis CotB-VP28 spores on black tiger shrimps (Penaeus monodon) against white spot syndrome virus infection. Oral administration of pellets coated with CotB-VP28 spores (≥1 × 10 CFU per g) conferred 75% protection after white spot syndrome virus challenge. Even after reducing CotB-VP28 spores in feed pellets to ≥1 × 10 CFU per g, 67·5% protections was still obtained. These data indicate that supplementation of CotB-VP28 spores at a low dose of ≥1 × 10 CFU per g could be effective in prophylaxis against white spot syndrome in black tiger shrimps.

Vaccines as alternatives to antibiotics for food producing animals. Part 2: new approaches and potential solutions

“…For shrimp farming, viral diseases have devastated the industry and one of the most important shrimp pathogens is white spot syndrome virus (WSSV) that causes seasonal outbreaks of disease [ 45 ]. A number of groups have developed B. subtilis spores that display the VP28 capsid protein of WSSV and when administered in feed appears to protect against white spot disease [ 46 – 49 ]. The mechanism for protection is intriguing; even though shrimp are not thought to produce antibodies, it is clear that presentation of the viral antigens does produce some level of specific immunity.…”

Effect of mannan oligosaccharides on the microbiota and productivity parameters of Litopenaeus vannamei shrimp under intensive cultivation in Ecuador

“…Other studies have provided evidence about the of microbiota´s function in host nutrition, specific tissues proliferation and immune mechanism regulation [17][18][19][20] . For example, bacterial strains derived from intestinal microbiota such as Pediococcus acidilactici [21][22][23] and Bacillus subtilis [24][25][26] have been tested in various aquaculture species and shown beneficial effects Thes.e advances have fostered a growing interest in microbiota modulation and their consequences on the productivity performance of animals under cultivation 9,10,27 . These supplements may be (i) probiotics, defined by Merrifield et al 28 as any microorganism supplied through the feed or culture water that benefits the host; (ii) prebiotics, defined by Bindels et al 29 as an indigestible compound which, via its metabolic breakdown by microorganisms in the intestine, acts on the profile and functionality of the intestinal microbiota, giving it a positive action on the host as an improvement of food growth and efficiency; or iii) symbiotic, which are defined by Gibson & Roberfroid (1995) 30 as nutritional supplements containing a prebiotic and a probiotic that beneficially influence the host by acting on the survival and implantation of live microorganisms as dietary supplements in the gastrointestinal tract.…”

The effectiveness of methanol extract and fractinations fromleaves of mangroveSonneratia albaand Bruguiera gymnorrhizafor the prevention of white spot syndrome virus (WSSV) infection in black tiger shrimp Penaeus monodon

“…Tackling white spot disease attack can be with by using probiotics (Lakshami et al, 2013;Pham et al, 2016;Sivasankar et al, 2017), vaccines (Nguyen et al, 2010;Amar et al, 2011;Syed & Kwang, 2011;Valdez et al, 2014;Chen et al, 2016), immunostimulants (Chen et al, 2010;Ermantianingrum et al, 2013;Velmurugan et al, 2013), and the management quality of the environment (Bosma et al, 2014), but until now, the mortality of shrimp in pond and hatchery caused by the disease attack is still occurring continously. The use of natural materials including mangrove and others associated with mangroves that used to tackle the disease in fish have started though it was still in laboratory scale as an antibacterial and antivirus.…”