iTRAQ-based proteomic analysis of defence responses triggered by the necrotrophic pathogen Rhizoctonia solani in cotton

Zhang, Min; Cheng, Shou-Ting; Wang, Haiyun; Wu, Jiahe; Luo, Yuanming; Wang, Qian; Wang, Fuxin; Xia, Gui-Xian

doi:10.1016/j.jprot.2016.11.011

Cited by 27 publications

(22 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We also noted that the VIGS- CYP71BE79 plants grown in the greenhouse frequently developed disease phenotypes (brown sunken lesions covering the hypocotyl–root junction) ( SI Appendix , Fig. S3 A and B ), similar to the symptoms caused by the soilborne necrotrophic fungus Rhizoctonia solani ( 29 ), whereas the control and other VIGS-treated plants did not. As PGF silencing blocked the whole gossypol biosynthesis pathway ( 25 ), the decreased amount of sesquiterpene phytoalexins in VIGS- CYP71BE79 plants was unlikely responsible for the enhanced susceptibility.…”

Section: Resultsmentioning

confidence: 80%

Characterization of gossypol biosynthetic pathway

Tian

Ruan

Huang

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

107

View full text Add to dashboard Cite

SignificanceCotton is an important crop, and terpenoids form the largest group of natural products. Gossypol and related sesquiterpene aldehydes in cotton function as phytoalexins against pathogens and pests but pose human health concerns, as cotton oil is still widely used as vegetable oil. We report the isolation and identification of four enzymes and the recharacterization of one previously reported P450. We are now close to the completion of the gossypol pathway, an important progress in agricultural and plant sciences, and the data are beneficial to improving food safety. Among the six compounds (intermediates) isolated following gene silencing, one affected plant disease resistance significantly. Thus, these “hidden natural products” harbor interesting biological activities worthy of exploration.

show abstract

Section: Resultsmentioning

confidence: 80%

Characterization of gossypol biosynthetic pathway

Tian

Ruan

Huang

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

107

View full text Add to dashboard Cite

show abstract

“…JAs are a class of lipid-derived hormones that regulate various defense responses against pathogens and insects (Wasternack and Hause, 2013 ; Zhang et al, 2017a ). Perception of a pathogen or insect invasion induces the synthesis of jasmonoyl-L-isoleucine (JA-Ile), which binds to the COI1-JAZ receptor, triggering the degradation of JAZ repressors and activates transcriptional reprogramming associated with plant defense (Zhang et al, 2017b ). In our study, two predicted WD-repeat proteins, Achn228601 and Achn388771, exhibited increased levels of accumulation in response to B. cinerea .…”

Section: Resultsmentioning

confidence: 99%

“…In general, proteomic analyses of plant hosts in response to fungal pathogens have been widely reported in recent years. For instance, Zhang et al ( 2017b ) employed an iTRAQ-based proteomic analysis of cotton to Rhizoctonia solani infection and reported that ROS homeostasis, epigenetic regulation, and phenylpropanoid biosynthesis were closely associated with innate immune responses in cotton. Kumar et al ( 2016 ) used a combined proteomic and metabolomic approach to characterize Fusarium oxysporum mediated metabolic reprogramming of chickpea roots.…”

Section: Introductionmentioning

confidence: 99%

Proteomic Analysis of Kiwifruit in Response to the Postharvest Pathogen, Botrytis cinerea

et al. 2018

View full text Add to dashboard Cite

Gray mold, caused by the fungus Botrytis cinerea, is the most significant postharvest disease of kiwifruit. In the present study, iTRAQ with LC-ESI-MS/MS was used to identify the kiwifruit proteins associated with the response to B. cinerea. A total of 2,487 proteins in kiwifruit were identified. Among them, 292 represented differentially accumulated proteins (DAPs), with 196 DAPs having increased, and 96 DAPs having decreased in accumulation in B. cinerea-inoculated vs. water-inoculated, control kiwifruits. DAPs were associated with penetration site reorganization, cell wall degradation, MAPK cascades, ROS signaling, and PR proteins. In order to examine the corresponding transcriptional levels of the DAPs, RT-qPCR was conducted on a subset of 9 DAPs. In addition, virus-induced gene silencing was used to examine the role of myosin 10 in kiwifruit, a gene modulating host penetration resistance to fungal infection, in response to B. cinerea infection. The present study provides new insight on the understanding of the interaction between kiwifruit and B. cinerea.

show abstract

“…This technique has been widely applied in proteome sequencing studies with high accuracy and reproducibility in recent years. Moreover, there have been many reports on the utilization of the iTRAQ technique to study changes in plant physiological metabolisms in response to abiotic stresses, such as cold [15][16][17], heat [18], and salt [19], and biotic stresses, including fungi [20,21], bacteria [22,23], and viruses [24,25].…”

Section: Introductionmentioning

confidence: 99%

iTRAQ-Based Proteomic Analysis of Watermelon Fruits in Response to Cucumber green mottle mosaic virus Infection

et al. 2020

IJMS

View full text Add to dashboard Cite

Cucumber green mottle mosaic virus (CGMMV) is an important viral pathogen on cucurbit plants worldwide, which can cause severe fruit decay symptoms on infected watermelon (usually called “watermelon blood flesh”). However, the molecular mechanism of this disease has not been well understood. In this study, we employed the isobaric tags for relative and absolute quantitation (iTRAQ) technique to analyze the proteomic profiles of watermelon fruits in response to CGMMV infection. A total of 595 differentially accumulated proteins (DAPs) were identified, of which 404 were upregulated and 191 were downregulated. Functional annotation analysis showed that these DAPs were mainly involved in photosynthesis, carbohydrate metabolism, secondary metabolite biosynthesis, plant–pathogen interaction, and protein synthesis and turnover. The accumulation levels of several proteins related to chlorophyll metabolism, pyruvate metabolism, TCA cycle, heat shock proteins, thioredoxins, ribosomal proteins, translation initiation factors, and elongation factors were strongly affected by CGMMV infection. Furthermore, a correlation analysis was performed between CGMMV-responsive proteome and transcriptome data of watermelon fruits obtained in our previous study, which could contribute to comprehensively elucidating the molecular mechanism of “watermelon blood flesh”. To confirm the iTRAQ-based proteome data, the corresponding transcripts of ten DAPs were validated by determining their abundance via quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). These results could provide a scientific basis for in-depth understanding of the pathogenic mechanisms underlying CGMMV-induced “watermelon blood flesh”, and lay the foundation for further functional exploration and verification of related genes and proteins.

show abstract

iTRAQ-based proteomic analysis of defence responses triggered by the necrotrophic pathogen Rhizoctonia solani in cotton

Cited by 27 publications

References 55 publications

Characterization of gossypol biosynthetic pathway

Characterization of gossypol biosynthetic pathway

Proteomic Analysis of Kiwifruit in Response to the Postharvest Pathogen, Botrytis cinerea

iTRAQ-Based Proteomic Analysis of Watermelon Fruits in Response to Cucumber green mottle mosaic virus Infection

Contact Info

Product

Resources

About