iTRAQ-Based Proteomic Analysis of Watermelon Fruits in Response to Cucumber green mottle mosaic virus Infection

Li, Xiaodong; Bi, Xinyue; An, Mengnan; Xia, Zihao; Wu, Yuanhua

doi:10.3390/ijms21072541

Cited by 17 publications

(18 citation statements)

References 57 publications

(69 reference statements)

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“…In terms of plant-virus interactions, plants have evolved immune systems that recognize the presence of viruses and initiate the expression of effective defense response factors. Recently, two studies have reported on transcriptome and proteomic profiles of CGMMV-infected and mock-inoculated watermelon leaves or fruit [ 19 , 29 ]. We compared them with our RNA-seq data and found that the number of DEGs identified in previous RNA-seq data (1641 DEGs) was similar to our study (1855 DEGs).…”

Section: Discussionmentioning

confidence: 99%

“…We compared them with our RNA-seq data and found that the number of DEGs identified in previous RNA-seq data (1641 DEGs) was similar to our study (1855 DEGs). Most of the DEGs or differentially accumulated proteins (DAPs) in the three studies were induced by CGMMV infection and functional analyses indicated they enriched in the pathways involved in stress response, photosynthesis, secondary metabolism, and so on [ 19 , 29 ]. These results further validated the common pathways in watermelon involved in CGMMV response.…”

Section: Discussionmentioning

confidence: 99%

“…Consequently, it is important to determine host response mechanisms of watermelon to CGMMV. Previously, the transcriptome, and proteomic, microRNA (miRNA), and virus derived small interfering RNAs (vsiRNAs) profiles via high-throughput sequencing have been performed, and some CGMMV-responsive genes, miRNAs, and vsiRNAs were identified in watermelon [ 17 – 19 ]. However, as the most important post-transcriptional mechanism, m 6 A modification profiles in watermelon following CGMMV infection have not been explored.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome-wide N6-methyladenosine (m6A) methylation in watermelon under CGMMV infection

Yao

et al. 2021

BMC Plant Biol

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Background Cucumber green mottle mosaic virus (CGMMV) causes substantial global losses in cucurbit crops, especially watermelon. N6-methyladenosine (m6A) methylation in RNA is one of the most important post-transcriptional modification mechanisms in eukaryotes. It has been shown to have important regulatory functions in some model plants, but there has been no research regarding m6A modifications in watermelon. Results We measured the global m6A level in resistant watermelon after CGMMV infection using a colorimetric method. And the results found that the global m6A level significantly decreased in resistant watermelon after CGMMV infection. Specifically, m6A libraries were constructed for the resistant watermelon leaves collected 48 h after CGMMV infection and the whole-genome m6A-seq were carried out. Numerous m6A modified peaks were identified from CGMMV-infected and control (uninfected) samples. The modification distributions and motifs of these m6A peaks were highly conserved in watermelon transcripts but the modification was more abundant than in other reported crop plants. In early response to CGMMV infection, 422 differentially methylated genes (DMGs) were identified, most of which were hypomethylated, and probably associated with the increased expression of watermelon m6A demethylase gene ClALKBH4B. Gene Ontology (GO) analysis indicated quite a few DMGs were involved in RNA biology and stress responsive pathways. Combined with RNA-seq analysis, there was generally a negative correlation between m6A RNA methylation and transcript level in the watermelon transcriptome. Both the m6A methylation and transcript levels of 59 modified genes significantly changed in response to CGMMV infection and some were involved in plant immunity. Conclusions Our study represents the first comprehensive characterization of m6A patterns in the watermelon transcriptome and helps to clarify the roles and regulatory mechanisms of m6A modification in watermelon in early responses to CGMMV.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transcriptome-wide N6-methyladenosine (m6A) methylation in watermelon under CGMMV infection

Yao

et al. 2021

BMC Plant Biol

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“…RPS proteins are linked to protein synthesis. RPS14 has been described to be up regulated in watermelon when challenged with cucumber green mottle mosaic virus [50]. It is known that a repression of protein synthesis is usually associated with the selective translation of mRNA that encode proteins that are crucial for stress recovery and thus cell survival.…”

Section: Several Daps Are Significantly Repressed At Both Time Pointsmentioning

confidence: 99%

Grapevine – Downy Mildew <em>Rendez-Vous</em>: Proteome Analysis of the First Hours of an Incompatible Interaction

Santos¹,

Nascimento²,

Coelho³

et al. 2020

Preprint

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Grapevine is one of the most relevant crops in the world being used for economically important products such as wine. Yet, relevant grapevine cultivars are heavily affected by diseases such as the downy mildew disease caused by Plasmopara viticola. Improvements on grapevine resistance are made mainly by breeding techniques where resistance traits are introgressed into cultivars with desired grape characteristics. However, there is still a lack of knowledge on how resistant or tolerant cultivars tackle the P. viticola pathogen. In this study, using a shotgun proteomics LC-MS/MS approach, we unravel the protein modulation of a highly tolerant grapevine cultivar, V. vinifera ‘Regent’, in the first hours post inoculation (hpi) with P. viticola. At 6 hpi, proteins related to defence and to response to stimuli are negatively modulated while at 12 hpi there is an accumulation of proteins belonging to both categories. The co-occurrence of effector-triggered susceptibility (ETS) and effector-triggered immunity (ETI) is detected at both time-points, showing that these defence processes present high plasticity. The results obtained in this study unravel the tolerant grapevine defence strategy towards P. viticola and may provide valuable insights on resistance associated candidates and mechanisms, which may play an important role in the definition of new strategies on breeding approaches.

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“…As powerful tools, proteomic technologies can also discern global protein expression changes during plant responses to biotic and abiotic stresses at the cellular and molecular levels. The Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) technology has been used in proteomics studies due to its high efficiency and accuracy in identifying differentially expressed proteins (DEPs) [ 32 , 33 , 34 ]. This technology is used to analyze proteomics in response to different biotic or abiotic stresses, including heat stress [ 35 ], salt stress [ 36 ], drought tolerance [ 37 ], rice response to Magnaporthe oryzae [ 38 ], defense response in cotton triggered by Rhizoctonia solani [ 39 ], and Arabidopsis response to Verticillium dahliae [ 40 ].…”

Section: Introductionmentioning

confidence: 99%

iTRAQ-Based Proteomics Analysis of Response to Solanum tuberosum Leaves Treated with the Plant Phytotoxin Thaxtomin A

Liu

Hao

Liu

et al. 2021

IJMS

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Thaxtomin A (TA) is a phytotoxin secreted by Streptomyces scabies that causes common scab in potatoes. However, the mechanism of potato proteomic changes in response to TA is barely known. In this study, the proteomic changes in potato leaves treated with TA were determined using the Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) technique. A total of 693 proteins were considered as differentially expressed proteins (DEPs) following a comparison of leaves treated with TA and sterile water (as a control). Among the identified DEPs, 460 and 233 were upregulated and downregulated, respectively. Based on Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses, many DEPs were found to be involved in defense and stress responses. Most DEPs were grouped in carbohydrate metabolism, amino acid metabolism, energy metabolism, and secondary metabolism including oxidation–reduction process, response to stress, plant–pathogen interaction, and plant hormone signal transduction. In this study, we analyzed the changes in proteins to elucidate the mechanism of potato response to TA, and we provided a molecular basis to further study the interaction between plant and TA. These results also offer the option for potato breeding through analysis of the resistant common scab.

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iTRAQ-Based Proteomic Analysis of Watermelon Fruits in Response to Cucumber green mottle mosaic virus Infection

Cited by 17 publications

References 57 publications

Transcriptome-wide N6-methyladenosine (m6A) methylation in watermelon under CGMMV infection

Transcriptome-wide N6-methyladenosine (m6A) methylation in watermelon under CGMMV infection

Grapevine – Downy Mildew <em>Rendez-Vous</em>: Proteome Analysis of the First Hours of an Incompatible Interaction

iTRAQ-Based Proteomics Analysis of Response to Solanum tuberosum Leaves Treated with the Plant Phytotoxin Thaxtomin A

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