Characterization of gossypol biosynthetic pathway

Tian, Xiu; Ruan, Ju-Xin; Huang, Jinquan; Yang, Changqing; Fang, Xin; Chen, Zhiwen; Hong, Hui; Wang, Ling-Jian; Mao, Ying‐Bo; Lu, Shan; Zhang, Tianzhen; Chen, Xiaoya

doi:10.1073/pnas.1805085115

Cited by 107 publications

(106 citation statements)

References 51 publications

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“…As plant metabolites provide indispensable resources for human nutrition, energy and medicine (Butelli et al ., ; Chen et al ., ), dissecting the mechanism of metabolite biosynthesis in plants draws extreme interest (Saito and Matsuda, ; Cardoso et al ., ; Quadrana et al ., ; Zhao et al ., ; Fernie and Tohge, ; Perchat et al ., ; Tian et al ., ). In recent years, the rapid development of analysis approaches for metabolomes and multiomics techniques have greatly improved our knowledge of the naturally occurring metabolic variation in plants and its underlying genetic determinants in several species (Keurentjes et al ., ; Shang et al ., ; Sadre et al ., ; Tohge et al ., ; Wen et al ., ; Fernie and Tohge, ; Rai et al ., ; Westhues et al ., ; Xiao et al ., ; Zhu et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Comparative analysis of metabolome of rice seeds at three developmental stages using a recombinant inbred line population

Wang

Gong

et al. 2019

The Plant Journal

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Plants are considered an important food and nutrition source for humans. Despite advances in plant seed metabolomics, knowledge about the genetic and molecular bases of rice seed metabolomes at different developmental stages is still limited. Here, using Zhenshan 97 (ZS97) and Minghui 63 (MH63), we performed a widely targeted metabolic profiling in seeds during grain filling, mature seeds and germinating seeds. The diversity between MH63 and ZS97 was characterized in terms of the content of metabolites and the metabolic shifting across developmental stages. Taking advantage of the ultra-high-density genetic map of a population of 210 recombinant inbred lines (RILs) derived from a cross between ZS97 and MH63, we identified 4681 putative metabolic quantitative trait loci (mQTLs) in seeds across the three stages. Further analysis of the mQTLs for the codetected metabolites across the three stages revealed that the genetic regulation of metabolite accumulation was closely related to developmental stage. Using in silico analyses, we characterized 35 candidate genes responsible for 30 structurally identified or annotated compounds, among which LOC_Os07g04970 and LOC_Os06g03990 were identified to be responsible for feruloylserotonin and L-asparagine content variation across populations, respectively. MetaboliteÀagronomic trait association and colocation between mQTLs and phenotypic quantitative trait loci (pQTLs) revealed the complexity of the metaboliteÀagronomic trait relationship and the corresponding genetic basis.

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Section: Discussionmentioning

confidence: 97%

Comparative analysis of metabolome of rice seeds at three developmental stages using a recombinant inbred line population

Wang

Gong

et al. 2019

The Plant Journal

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“…High‐resolution P 2p XPS spectra show two narrow peaks located at 129.6 and 130.5 eV, which are assigned to elemental P 2p 3/2 and 2p 1/2 (Figure S7 b) . A weak and broad sub‐band for oxidized phosphorus species (P x O y ) is apparent at around 133.2 eV, which is also confirmed by the corresponding FT‐IR spectra (Figure S8) . These results imply that partial oxidation happened on the surface of BP/RP, in accordance with its low zeta potential value of −39.2 mV (Table S2).…”

Section: Figurementioning

confidence: 99%

Direct Z‐Scheme Hetero‐phase Junction of Black/Red Phosphorus for Photocatalytic Water Splitting

et al. 2019

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Black phosphorus (BP) has recently drawn attention in photocatalysis for its optical properties. However, limited by the rapid recombination of photogenerated carriers, the use of BP for photocatalytic water splitting still remains a huge challenge. Herein, we prepare a black/red phosphorus (BP/RP) hetero‐phase junction photocatalyst by a wet‐chemistry method to promote the interfacial charge separation and thus achieve Z‐scheme photocatalytic water splitting without using sacrificial agents. The Z‐scheme mechanism was confirmed by time‐resolved transient absorption spectroscopy. This work provides a novel insight into the interface design of hetero‐phase junction with atomic precision.

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“…), the presence of gossypol can contribute to pathogen defense (Sunilkumar et al, 2006;Mao et al, 2007). Gossypol is synthesized via the mevalonate (MVA) and gossypol pathways (Heinstein et al, 1970;Chen et al, 1995Chen et al, , 1996Liu et al, 1999;Luo et al, 2001;Tian et al, 2018). Although the MVA pathway that converts acetyl-CoA to farnesyl pyrophosphate (FPP) is found in almost all plants, the conversion of FPP to hemigossypol in the gossypol pathway is exclusive to Gossypium.…”

Section: Introductionmentioning

confidence: 99%

Melatonin enhances cotton immunity to Verticillium wilt via manipulating lignin and gossypol biosynthesis

Zhang

et al. 2019

The Plant Journal

141

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SummaryPlants endure challenging environments in which they are constantly threatened by diverse pathogens. The soil‐borne fungus Verticillium dahliae is a devastating pathogen affecting many plant species including cotton, in which it significantly reduces crop yield and fiber quality. Melatonin involvement in plant immunity to pathogens has been reported, but the mechanisms of melatonin‐induced plant resistance are unclear. In this study, the role of melatonin in enhancing cotton resistance to V. dahliae was investigated. At the transcriptome level, exogenous melatonin increased the expression of genes in phenylpropanoid, mevalonate (MVA), and gossypol pathways after V. dahliae inoculation. As a result, lignin and gossypol, the products of these metabolic pathways, significantly increased. Silencing the serotonin N‐acetyltransferase 1 (GhSNAT1) and caffeic acid O‐methyltransferase (GhCOMT) melatonin biosynthesis genes compromised cotton resistance, with reduced lignin and gossypol levels after V. dahliae inoculation. Exogenous melatonin pre‐treatment prior to V. dahliae inoculation restored the level of cotton resistance reduced by the above gene silencing effects. Melatonin levels were higher in resistant cotton cultivars than in susceptible cultivars after V. dahliae inoculation. The findings indicate that melatonin affects lignin and gossypol synthesis genes in phenylpropanoid, MVA, and gossypol pathways, thereby enhancing cotton resistance to V. dahliae.

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Characterization of gossypol biosynthetic pathway

Cited by 107 publications

References 51 publications

Comparative analysis of metabolome of rice seeds at three developmental stages using a recombinant inbred line population

Comparative analysis of metabolome of rice seeds at three developmental stages using a recombinant inbred line population

Direct Z‐Scheme Hetero‐phase Junction of Black/Red Phosphorus for Photocatalytic Water Splitting

Melatonin enhances cotton immunity to Verticillium wilt via manipulating lignin and gossypol biosynthesis

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