Is really endogenous ghrelin a hunger signal in chickens? Association of GHSR SNPs with increase appetite, growth traits, expression and serum level of GHRL, and GH

El-Magd, Mohammed A.; Saleh, Ayman A.; Abdel-Hamid, Tamer M.; Saleh, Rasha M.; Afifi, Mohamed

doi:10.1016/j.ygcen.2016.08.016

Cited by 26 publications

(16 citation statements)

References 43 publications

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“…Gen GHSR pada lokasi rs16675844 mempunyai efek yang signifikan pada sifat konsumsi pakan dan konversi pakan, sedangkan gen GHSR pada lokasi rs14678932 menunjukkan adanya asosiasi yang signifikan pada sifat berat dada dan konsumsi pakan (Jin et al 2014). Gen GHSR pada ayam adalah reseptor ghrelin (GHRL), adapun gen GHSR adalah hormon peptida yang diproduksi oleh proventriculus ayam, yang merangsang pelepasan hormon pertumbuhan (GH) dan asupan makanan (El-Magd et al 2016). Gen GHSR dapat mempengaruhi pertumbuhan dan bobot karkas ayam karena gen tersebut mempunyai peranan penting dalam proses respon biologis, seperti perkembangan sel, proliferasi, diferensiasi, konsumsi pakan, dan keseimbangan energi (Tschop et al 2000).…”

Section: Genunclassified

“…Mutasi gen GHSR secara signifikan (P <0,05) berasoiasi dengan beberapa sifat perlemakan seperti kandungan protein kasar, bobot otot paha dan ketebalan lemak subkutan pada ayam dan bebek (Nie et al 2009). Pada penelitian yang dilakukan oleh El-Magd et al (2016) ditemukan mutasi lain gen GHSR pada exon 2 (G244A) yang berupa mutasi non-synonymous, adanya perubahan produk asam amino dari lisin menjadi arginin. Keragaman pada gen GHSR juga ditemukan pada penelitian yang dilakukan oleh Kaczor et al (2016), gen GHSR mempengaruhi bobot potong dan pertambahan bobot harian pada ayam broiler.…”

Section: Genunclassified

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Asosiasi Keragaman Growth Hormone Secretagogue Receptor (GHSR | Hin6i) terhadap Bobot Karkas Ayam Kampung pada Umur 12 Minggu

Syaikhullah¹,

Darwati²,

Sumantri³

2017

JIPTHP

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This study aimed to identify the GHSR genes polymorphism in chickens and to analyze the association of these genes on carcass weight and commercial cuts in chicken. A total of 60 local chickens (33 hen and 27 roosters) was used in this study. The chickens were bred in the colony cages and given full time access (ad libitum) to the commercial feed. The PCR-RFLP method was used to analyzed the gene polymorphism. The Hin6I was used as a restriction enzyme. The genotype frequency, allele frequency and Hardy Weinberg equilibrium were analyzed in this study. The phenotypic characteristics (body weight, carcass weight, breast weight, thigh weight, drumstick weight, wing weight and back weight) were analyzed using T test. The result showed that there were two genotypes (CT and TT) found in kampung chickens. The frequency of CT and TT genotype were 0.317 and 0.683, respectively. The value of Ho and He were 0.317 and 0.267 respectively. This population was not equal according to Hardy Weinberg equilibrium analysis. The GHSR gene polymorphism was significantly associated (P<0.05) with body weight, carcass weight, breast weight, thigh weight, drumstick weight, wing weight and back weight. The TT genotype was higher than CT genotype on all traits. The locus of GHSR|Hin6I could be used as a marker associated selection (MAS).

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Section: Genunclassified

Asosiasi Keragaman Growth Hormone Secretagogue Receptor (GHSR | Hin6i) terhadap Bobot Karkas Ayam Kampung pada Umur 12 Minggu

Syaikhullah¹,

Darwati²,

Sumantri³

2017

JIPTHP

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“…The poultry production is the quickest way to produce protein of high quality for human consumption. 1,2 Broiler chickens require about 3 kg of ration to create 1 kg of meat, but animals need 7 kg of food tom produce of red meat. 3 So in this inquiry, we enhance growth and immunity of broiler chickens to enhance meat production of good quality.…”

Section: Introductionmentioning

confidence: 99%

Effects of calf thymus extract and L-carnitine on immunity and growth performance of broiler chickens

2020

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Background: Increasing the bird’s wealth and increasing the growth rate are among the goals of increasing the animal wealth so; we studied the effects of L-carnitine and calf thymus extract on growth performance and immunity in broiler chickens.Methods: Eighty broiler chicks were divided into four groups, each group included twenty chicks. Group 1, was negative control with no treatment of feed or water. Group 2, the regular drinking water was replaced by L-carnitine-infused water (1 gram per liter). Group 3, the regular drinking water was replaced by calf thymus extract- infused water (1 ml per liter). Group 4, the regular drinking water was replaced by both L-carnitine and calf thymus extract- infused water. This treatment was administered from day 1 to day 30 of the cycle. Body weight, feed intake and feed conversion were assessed. The hemogram, leuckogram, total protein, globulin, albumin, phagocytic activity, phagocytic index and interleukin 2 were measured. Results: The hemogram and leuckogram parameters recorded a significant increase in treated groups compared to non treated group. The final body weight for all treatments was nearly the same, but slightly higher with application of L-carnitine. Phagocytic activity, index, TP, globulin and IL2 were significantly increased in treated groups.Conclusions: Both L-carnitine and thymus extract have significantly improved the general health condition, in addition, calf thymus extract improved not only the general body condition but also act has immunomodulatory effect which require more further studies.

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“…SNPs genotyping was done by SSCP which was carried out as previously described [18,19]. Animals were genotyped based on the pattern of SSCP bands.…”

Section: Snps Detection and Genotypingmentioning

confidence: 99%

Association of GHR Polymorphisms with Milk Production in Buffaloes

El-Komy

Saleh

Abdel-Hamid

et al. 2020

Animals

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For its role in the mediation of growth hormone (GH) galactopoietic effect, growth hormone receptor (GHR) was considered a functional candidate gene for milk performance in cattle. However, its genetic variation and potential effect have not been investigated in Egyptian buffaloes. This study aimed to screen GHR for polymorphisms and study their associations with milk traits in Egyptian buffaloes. Polymerase chain reaction, single-strand conformation polymorphism, and sequencing were used to identify mutations in 4 exons (E4–E6 and E8) of the GHR gene in 400 Egyptian buffaloes. No polymorphisms were found in E4, while 2 SNPs (c.380G>A/p.Arg127Lys and c.387C>T/p.Gly129) in E5, one silent mutation (c.435A>G/p.Pro145) in E6, and another missense mutation (c.836T>A/p.Phe279Tyr) in E8 were detected. The c.380G>A SNP in the extracellular domain was associated with milk yield, fat %, protein %, and 305-day milk, fat and protein yield, with higher levels in animals carrying the mutant A allele. The c.836T>A SNP in the transmembrane domain was associated with milk yield, fat %, protein %, and 305-day milk, fat and protein yield, with higher milk yield and lower fat %, protein %, fat and protein yield in the mutant A allele-animals. Interestingly, animals with the two mutant AA alleles produced higher milk yield, fat %, protein %, fat and protein yield, accompanied with upregulated expressions of GHR, GH, insulin-like growth factor 1 (IGF1), prolactin (PRL), prolactin receptor (PRLR), β-casein (encoded by CSN2 gene), and diacylglycerol acyltransferase-1 (DGAT1) genes and proteins in milk somatic cells. Therefore, selection of Egyptian buffaloes with mutant AA haplotypes for the novel c.380G>A SNP and the well-known c.836T>A SNP could improve milk yield and quality in buffaloes.

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Is really endogenous ghrelin a hunger signal in chickens? Association of GHSR SNPs with increase appetite, growth traits, expression and serum level of GHRL, and GH

Cited by 26 publications

References 43 publications

Asosiasi Keragaman Growth Hormone Secretagogue Receptor (GHSR | Hin6i) terhadap Bobot Karkas Ayam Kampung pada Umur 12 Minggu

Asosiasi Keragaman Growth Hormone Secretagogue Receptor (GHSR | Hin6i) terhadap Bobot Karkas Ayam Kampung pada Umur 12 Minggu

Effects of calf thymus extract and L-carnitine on immunity and growth performance of broiler chickens

Association of GHR Polymorphisms with Milk Production in Buffaloes

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