Asosiasi Keragaman Growth Hormone Secretagogue Receptor (GHSR | Hin6i) terhadap Bobot Karkas Ayam Kampung pada Umur 12 Minggu

Abstract

This study aimed to identify the GHSR genes polymorphism in chickens and to analyze the association of these genes on carcass weight and commercial cuts in chicken. A total of 60 local chickens (33 hen and 27 roosters) was used in this study. The chickens were bred in the colony cages and given full time access (ad libitum) to the commercial feed. The PCR-RFLP method was used to analyzed the gene polymorphism. The Hin6I was used as a restriction enzyme. The genotype frequency, allele frequency and Hardy Weinberg equilibrium were analyzed in this study. The phenotypic characteristics (body weight, carcass weight, breast weight, thigh weight, drumstick weight, wing weight and back weight) were analyzed using T test. The result showed that there were two genotypes (CT and TT) found in kampung chickens. The frequency of CT and TT genotype were 0.317 and 0.683, respectively. The value of Ho and He were 0.317 and 0.267 respectively. This population was not equal according to Hardy Weinberg equilibrium analysis. The GHSR gene polymorphism was significantly associated (P<0.05) with body weight, carcass weight, breast weight, thigh weight, drumstick weight, wing weight and back weight. The TT genotype was higher than CT genotype on all traits. The locus of GHSR|Hin6I could be used as a marker associated selection (MAS).

Exploring the Relationship Between Growth Hormone Secretagogue Receptor (GHSR) Gene and Body Proportions in Bangkok Chickens: Insights from DNA Sequencing and Hin6I Enzyme-Restricted PCR-RFLP Analysis

“…These results follow the study of Syaikhullah et al (2017), who found that the TT genotype was more dominant than the CT genotype in the body weight and carcass weight parameters of Kampung chickens. The GHSR gene at location rs16675844 had a significant effect on the characteristics of feed consumption and feed conversion, while the GHSR gene at location rs14678932 showed a significant association with the characteristics of breast weight and feed consumption (Jin et al, 2014;Kartikasari et al, 2019).…”

“…The GHSR gene amplification process produces a product of 470 bp (Figure 2). The results of this amplification were similar to the amplification process of the GHSR gene in Kampung chickens in the study of Syaikhullah et al (2017). The results of the amplification were then cut using the restriction enzyme Hin6I and produced 2 types of genotypes, namely CT and TT.…”

“…The intron also contains DNA sequences that bind enhancers that might affect the transcription process. Introns also contain regulatory RNA sequences that can affect the translation process and mRNA stability (Syaikhullah et al, 2017). When a mutation occurs, RNA can change the product of a gene.…”

“…The growth hormone secretagogue receptor (GHSR), or growth hormone secretagogue receptor, is involved in many physiological functions, including pituitary growth hormone secretion, food intake, and energy expenditure (Fang et al, 2010). The GHSR gene was identified as being associated with growth performance in kampung chickens (Syaikhullah et al, 2017). The GHSR gene is a gene that plays an important role as the main receptor in the process of GH secretion, or what is often called growth hormone (Kojima and Kangawa, 2005).…”