2016
DOI: 10.1021/acs.analchem.6b00502
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Bioassay Development for Ultrasensitive Detection of Influenza A Nucleoprotein Using Digital ELISA

Abstract: Flu is caused by the influenza virus that, due to mutations, keeps our body vulnerable for infections, making early diagnosis essential. Although immuno-based diagnostic tests are available, they have low sensitivity and reproducibility. In this paper, the prospect of detecting influenza A virus using digital ELISA has been studied. To appropriately select bioreceptors for this bioassay, seven commercial antibodies against influenza A nucleoprotein were methodically tested for their reactivity and binding affi… Show more

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Cited by 93 publications
(85 citation statements)
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“…Instead, it is the off rate of detection antibodies targeting the analyte bound on a bead that determines the sensing performance and detection limits for the SiMoA. This has also been supported by other studies investigating the dependence of analytical performance of digital single molecule sensors upon the binding kinetics (on‐ and off‐rates) of capture and detection antibodies . When one factors the confinement in the well in the SiMoA experiment and the pore in the nanopore blockade experiment, where the analyte molecule may not be able to diffuse away at all, then the apparent unlimited lifetime of single molecule binding pairs becomes understandable.…”
Section: How Nanoparticles Solve the Challenges Faced By Single Molecmentioning
confidence: 85%
“…Instead, it is the off rate of detection antibodies targeting the analyte bound on a bead that determines the sensing performance and detection limits for the SiMoA. This has also been supported by other studies investigating the dependence of analytical performance of digital single molecule sensors upon the binding kinetics (on‐ and off‐rates) of capture and detection antibodies . When one factors the confinement in the well in the SiMoA experiment and the pore in the nanopore blockade experiment, where the analyte molecule may not be able to diffuse away at all, then the apparent unlimited lifetime of single molecule binding pairs becomes understandable.…”
Section: How Nanoparticles Solve the Challenges Faced By Single Molecmentioning
confidence: 85%
“…Only one antibody was found to capture the nucleoprotein directly in the lysis buffer. Results provide another alternative for the ultrasensitive immunoassay-based detection of influenza virus (Leirs et al, 2016).…”
Section: Spr and Elisa Combined Ultrasensitive Detection Of Influenza Amentioning
confidence: 99%
“…Although rapid detection of this virus is mandatory, present influenza diagnosis techniques cannot compete with the mutagenic behavior of the influenza virus. For example, viral culture, which was accepted as a golden standard method, needs two or three days to retrieve the results which is a kind of long time for the diagnosis of influenza virus [18][19][20] . On the other hand, quantitative polymerase chain reaction (qPCR), which is chosen frequently by the health personnel can be described as sensitive but relatively slow (took about 2 h).…”
Section: Introductionmentioning
confidence: 99%
“…On the other hand, quantitative polymerase chain reaction (qPCR), which is chosen frequently by the health personnel can be described as sensitive but relatively slow (took about 2 h). qPCR is also expensive and can be performed only with the specialists [20][21][22] . Apart from these methods, various immune based rapid tests have been fabricated recently to compensate the fast diagnosis need of the virus.…”
Section: Introductionmentioning
confidence: 99%
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