DNA Barcoding of genus Hexacentrus in China reveals cryptic diversity within Hexacentrus japonicus (Orthoptera, Tettigoniidae)

Abstract

DNA barcoding has been proved successful to provide resolution beyond the boundaries of morphological information. Hence, a study was undertaken to establish DNA barcodes for all morphologically determined Hexacentrus species in China collections. In total, 83 specimens of five Hexacentrus species were barcoded using standard mitochondrial cytochrome c oxidase subunit I (COI) gene. Except for Hexacentrus japonicus, barcode gaps were present in the remaining Hexacentrus species. Taxon ID tree generated seven BOLD’s barcode index numbers (BINs), four of which were in agreement with the morphological species. For Hexacentrus japonicus, the maximum intraspecific divergence (4.43%) produced a minimal overlap (0.64%), and 19 specimens were divided into three different BINs. There may be cryptic species within the current Hexacentrus japonicus. This study adds to a growing body of DNA barcodes that have become available for katydids, and shows that a DNA barcoding approach enables the identification of known Hexacentrus species with a very high resolution.

DNA barcoding and a new taxonomic status of the Triaenodes ochreellus lefkas Malicky, 1974 (Insecta, Trichoptera) with new distribution data

“…DNA barcoding is a very useful method in the study of biodiversity, taxonomy, phylogeny and phylogeography of different groups of organisms (e.g. Brehm et al 2019;Cárdenas et al 2013;Elías-Gutiérrez et al 2008;Guo et al 2016;Huemer et al 2020;Léger et al 2020;Tyagi et al 2017;Yang et al 2016), including Trichoptera (Kučinić et al 2016(Kučinić et al , 2017(Kučinić et al , 2019aSzivák et al 2017;Santos et al 2016;Valladolid et al 2018Valladolid et al , 2019Vitecek et al 2020). In many studies, DNA barcoding has aided morphological identification of different taxa (Ćukušić et al 2017;Kučinić et al 2019bKučinić et al , 2020, but also enabled the discovery of new species (Brehm et al 2019;Dela Cruz et al 2016;Graf et al 2012;Kučinić et al 2013;Léger et al 2020;Tyagi et al 2017;Vaglia et al 2008).…”

Springs: DNA barcoding of caddisflies (Insecta, Trichoptera) in Croatia with notes on taxonomy and conservation biology

“…Each year thousands of new species within various groups of organisms are described, and the introduction of DNA barcoding based on the cytochrome c oxidase subunit 1 mitochondrial gene (mtCOI), along with the establishment of the Barcode of Life Data Systems (BOLD) (Hebert et al, 2003a(Hebert et al, , 2003bRatnasingham & Hebert, 2007) resulted in new aspects of global biodiversity on Earth. DNA barcoding has proved to be a useful method in studies of the taxonomy, phylogenesis, phylogeography and biodiversity of different groups of organisms (for example Amora et al 2015;Brehm et al, 2019;Cárdenas et al, 2013;De Barros Machado et al, 2017;Dela Cruz et al, 2016;Elías-Gutiérrez et al, 2008;Guo et al, 2016;Huemer et al, 2020;Kučinić et al, 2019aKučinić et al, , 2019bLéger et al, 2020;Pauls et al, 2009;Santos et al 2016;Tyagi et al, 2017;Vaglia et al 2008;Vijayan & Tsou, 2010;Yang et al, 2015).…”