Antioxidative activities of 62 wild mushrooms from Nepal and the phenolic profile of some selected species

Tamrakar, Sonam; Tran, Hai Bang; Nishida, Marina; Kaifuchi, Satoru; Suhara, Hiroto; Doi, Kunio; Fukami, Katsuya; Parajuli, Gopal Prasad; Shimizu, Kuniyoshi

doi:10.1007/s11418-016-1013-1

Cited by 16 publications

(10 citation statements)

References 35 publications

(41 reference statements)

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“…Phenolic compounds are an important group of compounds responsible for the antibacterial activity of mushrooms [27]. Previous investigations of the total phenolic content of the ethanol extracts of Nepalese wild mushrooms [13,14] showed very high phenolic content in I. clemensiae, I. andersonii, I. cuticularis, C. setiporus and Pseudomerulius curtisii. However, the very low phenolic content in P. stiptica, M. mucida, Gymnopus sp.…”

Section: Discussionmentioning

confidence: 99%

“…Ninety different samples were investigated in this study. The botanical origin, location, habitat, and identification of the samples have been described in previously published reports [13,14].…”

Section: Mushroom Samplesmentioning

confidence: 99%

“…The exploration of the therapeutic potential and the cultivation techniques of the indigenous wild mushrooms could add a new dimension to the Nepalese mushroom market and functional food industry. However, apart from some studies on the antioxidant activities [13,14], other biological activities of Nepalese mushrooms remain largely unstudied. Therefore, in an attempt to fill this gap, the current research explores the antibacterial property of the ethanol extracts of 90 Nepalese wild mushrooms samples against S. aureus and P. acnes.…”

Section: Introductionmentioning

confidence: 99%

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Antibacterial activity of Nepalese wild mushrooms against Staphylococcus aureus and Propionibacterium acnes

et al. 2017

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The present study aims to reveal the antibacterial potential of the wild mushrooms of Nepal. Despite the recognition of the medicinal potential of the natural resources in this country, a systematic study on the bioactivities of the wild mushrooms is still lacking. Therefore, in an attempt to fill this gap, ethanol extracts of 90 Nepalese wild mushroom samples were tested for antibacterial activity against Staphylococcus aureus and Propionibacterium acnes. Staphylococcus aureus was comparatively more susceptible with Inonotus clemensiae exhibiting the least minimum bactericidal concentration (MBC) of 100 lg/mL. The major compound in I. clemensiae was identified to be hispidin using high resolution liquid chromatography-electron spray ionization mass spectrometry (LC-ESI-MS) and nuclear magnetic resonance (NMR) analyses. Furthermore, the minimum inhibitory concentration (MIC) and MBC values of hispidin were determined to be 25 and 100 lg/mL for S. aureus and P. acnes, respectively. These findings show that the Nepalese wild mushrooms have the potential to be a novel addition to the functional ingredients industry due to their strong antibacterial potential. Keywords Wild mushrooms Á Nepal Á Antibacterial activity Á Inonotus clemensiae Á Hispidin Electronic supplementary material The online version of this article (

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Section: Discussionmentioning

confidence: 99%

Section: Mushroom Samplesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antibacterial activity of Nepalese wild mushrooms against Staphylococcus aureus and Propionibacterium acnes

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“…There are also reducing agents like ascorbic acid and flavonoids contributing in lower values [88, 89]. Phenolic components are potential antioxidants which can donate hydrogen to free radicals to terminate the chain reaction of lipid oxidation at the initial stage.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Potential, Identification and Phylogenetic Affiliation of Wild Mushrooms from Two Sub-Tropical Semi-Evergreen Indian Forest Ecosystems

Lallawmsanga¹,

Passari²,

Mishra³

et al. 2016

PLoS ONE

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The diversity of wild mushrooms was investigated from two protected forest areas in India and 231 mushroom specimens were morphologically identified. Among them, 76 isolates were screened for their antimicrobial potential against seven bacterial and fungal pathogens. Out of 76 isolates, 45 isolates which displayed significant antimicrobial activities were identified using ITS rRNA gene amplification and subsequently phylogenetically characterized using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. Sequencing of the ITS rRNA region classified the isolates into 16 genera belonging to 11 families. In total, 11 RAPD and 10 ISSR primers were selected to evaluate genetic diversity based on their banding profile produced. In total 337 RAPD and 312 ISSR bands were detected, among which percentage of polymorphism ranges from 34.2% to 78.8% and 38.6% to 92.4% by using RAPD and ISSR primers respectively. Unweighted Pair-Group Method with Arithmetic Mean (UPGMA) trees of selected two methods were structured similarly, grouping the 46 isolates into two clusters which clearly showed a significant genetic distance among the different strains of wild mushroom, with an similarity coefficient ranges from 0.58 to 1.00 and 0.59 to 1.00 with RAPD and ISSR analysis respectively. This reporthas highlighted both DTR and MNP forests provide a habitat for diverse macrofungal species, therefore having the potential to be used for the discovery of antimicrobials. The report has also demonstrated that both RAPD and ISSR could efficiently differentiate wild mushrooms and could thus be considered as efficient markers for surveying genetic diversity. Additionally, selected six wild edible mushroom strains (Schizophyllum commune BPSM01, Panusgiganteus BPSM27, Pleurotussp. BPSM34, Lentinussp. BPSM37, Pleurotusdjamor BPSM41 and Lentinula sp. BPSM45) were analysed for their nutritional (proteins, carbohydrates, fat and ash content), antioxidant potential. The present findings also suggested that the wild edible mushroom strains do not have only nutritional values but also can be used as an accessible source of natural antioxidants.

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“…It has been previously reported that constituent compounds of macrofungi have cytotoxic, antinociceptic, and immunomodulatory properties. 2,3,4 Alternative antibacterials and antioxidants have been found in compounds from the environment. The growing potentials of mushrooms have also encompassed such properties as it has been confirmed that its chemical components also have antibacterial 5 and antioxidant activities by radical and metal-scavenging.…”

Section: Introductionmentioning

confidence: 99%

Anti-Staphylococcal and Antioxidant Properties of Crude Ethanolic Extracts of Macrofungi Collected from the Philippines

Gaylan¹,

Estebal²,

Tantengco³

et al. 2017

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Introduction:Macrofungi have been used in the Philippines as source of food and traditional medicines. However, these macrofungi in the Philippines have not yet been studied for different biological activities. Thus, this research determined the potential antibacterial and antioxidant activities of crude ethanolic extracts of seven macrofungi collected in Bataan, Philippines. Methods: Kirby-Bauer disk diffusion assay and broth microdilution method were used to screen for the antibacterial activity and DPPH scavenging assay for the determination of antioxidant activity. Results: F. rosea, G. applanatum, G. lucidum and P. pinisitus exhibited zones of inhibition ranging from 6.55 ± 0.23 mm to 7.43 ± 0.29 mm against S. aureus, D. confragosa, F. rosea, G. lucidum, M. xanthopus and P. pinisitus showed antimicrobial activities against S. aureus with an MIC 50 ranging from 1250 μg/mL to 10000 μg/mL. F. rosea, G. applanatum, G. lucidum, M. xanthopus exhibited excellent antioxidant activity with F. rosea having the highest antioxidant activity among all the extracts tested (3.0 μg/mL). Conclusion: Based on the results, these Philippine macrofungi showed antistaphylococcal activity independent of the antioxidant activity. These can be further studied as potential sources of antibacterial and antioxidant compounds. Key words: Antimicrobial, Antioxidant, Mushrooms, Philippines, Staphylococcus aureus. 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging were performed using the mushroom extracts to determine the species with potential bioactive molecules of pharmaceutical importance. MATERIALS AND METHODS Collection of mushroom speciesThe mushroom species were collected from three sites Sitio Pastolan, Hermosa from July to December of 2016. The map coordinate of the study site is 14°50'26.29''N and 120°20'47.17''E. Collected macrofungi were wrapped in newspaper. Each were labeled accordingly and voucher specimen were submitted to the National Museum of the Philippines for taxonomic identification. Preparation of ethanol mushroom extractsMacrofungi were weighed separately using a toploading balance. They were dried in the oven overnight at 40 o C and were air-dried after for seven days. They were washed with distilled water and cut into small pieces. About 150 grams of each macrofungus were soaked in 500 mL of 95% ethanol for five days. Filtration was done using Whatman filter paper No. 107Where Ac is the absorbance of the control and As is the absorbance with the extract. Tests were done in triplicates and a calibration curve was constructed with ascorbic acid as the standard. Data analysisData were analyzed using the SPSS program and GraphPad Prism. Half maximal effective concentration (EC 50 ) was also computed from the SA values of each ethanolic extract. Shapiro-Wilk test was performed to determine if the data were in normal distribution. Mann-Whitney's test was done to determine which sample extract has significantly different antimicrobial activity when paired with ciprofloxacin. The test was also done to assess d...

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Antioxidative activities of 62 wild mushrooms from Nepal and the phenolic profile of some selected species

Cited by 16 publications

References 35 publications

Antibacterial activity of Nepalese wild mushrooms against Staphylococcus aureus and Propionibacterium acnes

Antibacterial activity of Nepalese wild mushrooms against Staphylococcus aureus and Propionibacterium acnes

Antimicrobial Potential, Identification and Phylogenetic Affiliation of Wild Mushrooms from Two Sub-Tropical Semi-Evergreen Indian Forest Ecosystems

Anti-Staphylococcal and Antioxidant Properties of Crude Ethanolic Extracts of Macrofungi Collected from the Philippines

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