MicroRNA-126 inhibits tumor cell invasion and metastasis by downregulating ROCK1 in renal cell carcinoma

Abstract

MicroRNAs (miRNAs) are involved in cancer development and progression. Renal cell carcinoma (RCC) frequently undergoes metastasis and has a high mortality rate. The current study measured miRNA-126 (miR-126) expression levels in 128 pairs of clear cell RCC and adjacent normal kidney tissue samples by reverse transcription-quantitative polymerase chain reaction, and analyzed the association between miR-126 and various clinicopathological parameters. In addition, cell proliferation, wound healing and cell invasion assays were conducted using RCC cells overexpressing miR-126. Potential miR-126 target genes and the signaling pathways that may be regulated by miR-126 were then examined. miR-126 expression was significantly reduced in patients with metastatic RCC compared with patients without metastasis. Consistently, overexpression of miR-126 in RCC cells significantly inhibited cell proliferation, migration and invasion in vitro compared with negative control miRNA. A luciferase reporter assay demonstrated that miR-126 targets Rho associated coiled-coil containing protein kinase 1 (ROCK1) by directly binding the 3′-untranslated region. Furthermore, western blotting identified miR-126 as an important regulator of the AKT and extracellular signal-regulated 1/2 signaling pathways. The results of the present study indicate that miR-126 inhibits RCC cell proliferation, migration and invasion by downregulating ROCK1. These findings suggest that miR-126 may be valuable as a potential target for therapeutic intervention in RCC.

Exosomes Derived From Mesenchymal Stem Cells Modulate miR-126 to Ameliorate Hyperglycemia-Induced Retinal Inflammation Via Targeting HMGB1

“…MiR-126 is considered an endothelial cellrestricted miRNA that mediates inflammation and vascular development and has been reported as an important therapeutic treatment for various diseases involving aberrant inflammation and vascular leakage. 16 A report from our laboratory has demonstrated that the miR-126 pathway may contribute to ameliorating DR through its ability to enhance vascular repair and reduce inflammatory effects. 17 Previous research also showed that miR-126 plays a key role in modulating the expression of inflammatory factors after hyperglycemia-induced retinal inflammation.…”

Estrogen affects cell growth and IGF-1 receptor expression in renal cell carcinoma

“…Western blotting analysis was performed as described previously. 15 Briefly, cells were harvested using the CelLytic extraction kit supplemented with protease inhibitors. Protein concentration was measured using the BCA Protein Assay reagent kit (Thermo Fisher Scientific) according to the manufacturer’s instructions.…”

Research Article Expression of microRNAs miR-126 and miR-873 and genes CASPASE-8 and C-FLIP in neurospheres of Glioblastoma lines U-343 subjected to treatment with ionizing radiation and temozolomide.

“…Jiang et al (2014) concluded that miR-126 inhibits cell growth, invasion and migration of osteosarcoma cells. Zhang et al (2016) concluded in their study an association of miR-126 with CRC progression and metastasis, thus suggesting that this microRNA may function as a tumor suppressor involved in the development of CRC, being a potential biomarker in the diagnosis and therapeutic act.…”