Research Article Expression of microRNAs miR-126 and miR-873 and genes CASPASE-8 and C-FLIP in neurospheres of Glioblastoma lines U-343 subjected to treatment with ionizing radiation and temozolomide.

Abstract

Glioblastoma is considered incurable, even with a combination of therapies (chemo and radiotherapy), and surgical resection. New therapeutic approaches are needed to improve the prognosis of patients with glioblastoma. In recent decades, research has focused on molecular biology of brain tumors. We examined the role of programmed cell death, apoptosis, through two microRNAs that act as possible pro and anti-apoptotic gene regulators. We evaluated the expression of the genes CASPASE-8 and C-FLIP and microRNAs miR-126-5p and miR-873-5p in adhered cells (AC) and neurospheres (NS) from cell line U343, which were submitted to temozolomide (TMZ) and ionizing radiation (IR), isolated and associated (TMZ + IR). We concluded that microRNA-126 and 873 were differentially expressed as as a function of treatment regime in glioblastomas. The higher expression of the caspase-8 gene in adhered cells in the group treated with IR when compared to the other groups at time 48 h suggests that the ionizing radiation in the adhered cells of the glioblastoma cell line culture has apoptosis-inducing properties.