Long‐term skin regeneration in xenografts from <scp>iPSC</scp> teratoma‐derived human keratinocytes

García, Marta; Quintana-Bustamante, Óscar; Segovia, José C.; Bueren, Juan A.; Martínez-Santamaría, Lucía; Guerrero-Aspizua, Sara; Escámez, M.J.; Río, Marcela Del; Larcher, Fernando

doi:10.1111/exd.13049

Cited by 4 publications

(2 citation statements)

References 8 publications

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“…As source of KC, different approaches have been described: KC isolated from human skin, 30,33 KC derived from human embryonic stem cells (hESC) 34 or induced pluripotent stem cell (iPSC) teratoma-derived KC. 35 Moreover, it has been shown that the Rho kinase inhibitor Y-27632 is able to prolong the life span of adult NHEK while maintaining their ability to form HSEs. This indication could aid in generating large quantities of KC required to meet the increasing need for HSEs, 36 as the immortalized keratinocyte cell line HaCaT has been found inadequate to form a proper epidermis in skin equivalents.…”

Section: Development Of Epidermal Skin Equivalents: From Normal To Psoriatic Modelsmentioning

confidence: 99%

In vitro psoriasis models with focus on reconstructed skin models as promising tools in psoriasis research

Desmet

Ramadhas

Lambert

et al. 2017

Exp Biol Med (Maywood)

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Psoriasis is a complex chronic immune-mediated inflammatory cutaneous disease associated with the development of inflammatory plaques on the skin. Studies proved that the disease results from a deregulated interplay between skin keratinocytes, immune cells and the environment leading to a persisting inflammatory process modulated by pro-inflammatory cytokines and activation of T cells. However, a major hindrance to study the pathogenesis of psoriasis more in depth and subsequent development of novel therapies is the lack of suitable pre-clinical models mimicking the complex phenotype of this skin disorder. Recent advances in and optimization of three-dimensional skin equivalent models have made them attractive and promising alternatives to the simplistic monolayer cultures, immunological different in vivo models and scarce ex vivo skin explants. Moreover, human skin equivalents are increasing in complexity level to match human biology as closely as possible. Here, we critically review the different types of three-dimensional skin models of psoriasis with relevance to their application potential and advantages over other models. This will guide researchers in choosing the most suitable psoriasis skin model for therapeutic drug testing (including gene therapy via siRNA molecules), or to examine biological features contributing to the pathology of psoriasis. However, the addition of T cells (as recently applied to a de-epidermized dermis-based psoriatic skin model) or other immune cells would make them even more attractive models and broaden their application potential. Eventually, the ultimate goal would be to substitute animal models by three-dimensional psoriatic skin models in the pre-clinical phases of anti-psoriasis candidate drugs. Impact statement The continuous development of novel in vitro models mimicking the psoriasis phenotype is important in the field of psoriasis research, as currently no model exists that completely matches the in vivo psoriasis skin or the disease pathology. This work provides a complete overview of the different available in vitro psoriasis models and suggests improvements for future models. Moreover, a focus was given to psoriatic skin equivalent models, as they offer several advantages over the other models, including commercial availability and validity. The potential and reported applicability of these models in psoriasis pre-clinical research is extensively discussed. As such, this work offers a guide to researchers in their choice of pre-clinical psoriasis model depending on their type of research question.

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Section: Development Of Epidermal Skin Equivalents: From Normal To Psoriatic Modelsmentioning

confidence: 99%

In vitro psoriasis models with focus on reconstructed skin models as promising tools in psoriasis research

Desmet

Ramadhas

Lambert

et al. 2017

Exp Biol Med (Maywood)

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“…It has been proved that there is a possibility for iPSCs directly differentiated into endothelial cells [ 14 , 15 ] or keratinocytes [ 16 , 17 ]. For inducing the differentiation of MSCs into epidermoid cells, coculture with keratinocytes is a common method [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Endowing iPSC-Derived MSCs with Angiogenic and Keratinogenic Differentiation Potential: A Promising Cell Source for Skin Tissue Engineering

Lin

Miao

et al. 2018

BioMed Research International

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Induced pluripotent stem cells (iPSC) hold tremendous potential for personalized cell-based therapy for skin regeneration. Aiming to establish human iPSCs as a potential cell source for skin tissue engineering, we expect to obtain an epidermal-like cell line with angiogenic and keratinogenic differentiation potential via inducing iPSC-derived mesenchymal stem cells (iPSC-MSCs) with basic fibroblast growth factor (bFGF) and/or keratinocyte growth factor (KGF). The results show that iPSC-MSCs were successfully induced with a positive FGFR/KGFR expression on the cell surface. BFGF/KGF induction could significantly increase the expression of vascularization marker CD31 and keratinization marker CK10, respectively, while when combined together, although CD31 and CK10 were still positively expressed, their expressions were lower than that of the single induction group, suggesting that the effects of the two growth factors interfered with each other. This cell line with angiogenic and keratinogenic differentiation potential provides a promising new source of cells for the construction of well vascularized and keratinized tissue engineered skin, furthermore establishing an effective strategy for iPSC-based therapy in skin tissue engineering.

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Induced pluripotent stem cells to generate skin tissue models

Kashpur

Smith

Mukhamedshina

et al. 2018

Skin Tissue Models for Regenerative Medicine

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Long‐term skin regeneration in xenografts from iPSC teratoma‐derived human keratinocytes

Cited by 4 publications

References 8 publications

In vitro psoriasis models with focus on reconstructed skin models as promising tools in psoriasis research

In vitro psoriasis models with focus on reconstructed skin models as promising tools in psoriasis research

Endowing iPSC-Derived MSCs with Angiogenic and Keratinogenic Differentiation Potential: A Promising Cell Source for Skin Tissue Engineering

Induced pluripotent stem cells to generate skin tissue models

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