Endowing iPSC-Derived MSCs with Angiogenic and Keratinogenic Differentiation Potential: A Promising Cell Source for Skin Tissue Engineering

Lin, Weimin; Miao, Chang; Hu, Chen; Qin, Siyu; Chu, Chenyu; Xiang, Lin; Man, Yi; Qu, Yang

doi:10.1155/2018/8459503

Cited by 14 publications

(8 citation statements)

References 34 publications

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“…We speculated that this is because the concentration of released growth factors increases as the concentration of PRP and LyPRP increased. With prolonged culturing time, the cells tended to proliferate rather than differentiate, so the expression of osteogenic differentiation related genes is slightly reduced (W. Lin et al, ; Shirai, Ishisaki, Kaku, Tamura, & Furuichi, ).…”

Section: Discussionmentioning

confidence: 99%

Effects of PRP and LyPRP on osteogenic differentiation of MSCs

Xie

Chen

et al. 2019

J Biomedical Materials Res

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Platelet-rich plasma (PRP) is rich in a variety of growth factors and plays an important role in the proliferation and differentiation of mesenchymal stem cells (MSCs). It has been reported that the preparation of freeze-dried platelets (lyophilized platelets [LyPRP]) from platelets could be an effective strategy to preserve the bioactivity of platelets for a long time. In this study, the osteogenic induction effects of PRP and LyPRP on MSCs were evaluated. The rabbit arterial blood was drawing to preparation of PRP by secondary centrifugation. Whole blood was prepared by lyophilization buffer to prepare LyPRP, which were activated by chloride and their surface morphology was observed. It was observed using a scanning electron microscope that platelets were evenly distributed on the surface of PRP and LyPRP. Growth factors were slowly released from PRP and LyPRP during the first 7 days and detected by the enzyme-linked immunosorbent assay kit. Cell proliferation assays and fluoresceindiacetate/propidium iodide (FDA/PI) staining demonstrated that PRP and LyPRP could promote cell proliferation. PRP and LyPRP were also shown to promote osteogenic differentiation of MSCs in vitro by osteogenesis characteristic staining and qPCR quantitative detection of osteogenic related gene expression. Both PRP and LyPRP could promote the proliferation and osteogenic differentiation of MSCs effectively. Moreover, PRP exhibited a better osteogenic induction effect on MSC than LyPRP. K E Y W O R D S BMSCs, LyPRP, osteogenic induction, PRP

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Section: Discussionmentioning

confidence: 99%

Effects of PRP and LyPRP on osteogenic differentiation of MSCs

Xie

Chen

et al. 2019

J Biomedical Materials Res

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“…iPSCs are a new class of pluripotent stem cells which can differentiate into cells of all three germ layers 58 . These cells are generated by reprogramming somatic cells (e.g., dermal fibroblasts) via exposure to four specific genes encoding transcription factors, that is, octamer 3/4 (Oct3), sex‐determining region Y‐box (Sox2), Kruppel‐like factor 4 (Klf4), and v‐Myc myelocytomatosis viral oncogene homologue (c‐Myc) 59 .…”

Section: Induced Pluripotent Stem Cellsmentioning

confidence: 99%

“…iPSCs are a new class of pluripotent stem cells which can differentiate into cells of all three germ layers. 58 These cells are generated by reprogramming somatic cells (e.g., dermal fibroblasts) via exposure to four specific genes encoding transcription factors, that is, T A B L E 2 A short list of experimental studies in which different stem cells were used for promoting angiogenesis and wound healing. oped iPSCs from postnatal somatic cells.…”

Section: Induced Pluripotent Stem Cellsmentioning

confidence: 99%

Stem cell‐mediated angiogenesis in skin tissue engineering and wound healing

Azari

Nazarnezhad

Webster

et al. 2022

Wound Repair Regeneration

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The timely management of skin wounds has been an unmet clinical need for centuries.While there have been several attempts to accelerate wound healing and reduce the cost of hospitalisation and the healthcare burden, there remains a lack of efficient and effective wound healing approaches. In this regard, stem cell-based therapies have garnered an outstanding position for the treatment of both acute and chronic skin wounds. Stem cells of different origins (e.g., embryo-derived stem cells) have been utilised for managing cutaneous lesions; specifically, mesenchymal stem cells (MSCs) isolated from foetal (umbilical cord) and adult (bone marrow) tissues paved the way to more satisfactory outcomes. Since angiogenesis plays a critical role in all four stages of normal wound healing, recent therapeutic approaches have focused on utilising stem cells for inducing neovascularisation. In fact, stem cells can promote angiogenesis via either differentiation into endothelial lineages or secreting pro-angiogenic exosomes.Furthermore, particular conditions (e.g., hypoxic environments) can be applied in order to boost the pro-angiogenic capability of stem cells before transplantation. For tissue engineering and regenerative medicine applications, stem cells can be combined with specific types of pro-angiogenic biocompatible materials (e.g., bioactive glasses) to enhance the neovascularisation process and subsequently accelerate wound healing.As such, this review article summarises such efforts emphasising the bright future that is conceivable when using pro-angiogenic stem cells for treating acute and chronic skin wounds.

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“…Cell‐based therapy offers a promising strategy for tissue regeneration (Lin et al, ; Mount, Ward, Kefalas, & Hyllner, ). Compared with cells alone, 3D biomaterials can serve as ideal scaffolds for cell delivery, protecting cells from injection shear, providing structural support, and controlling ideal cell fate, thereby improving the outcome of cell‐based therapy (Khademhosseini & Langer, ).…”

Section: Scaffolds With Encapsulated Cellsmentioning

confidence: 99%

Three‐dimensional electrospun nanofibrous scaffolds for bone tissue engineering

et al. 2019

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Electrospinning technology has been widely used in the past few decades to prepare nanofibrous scaffolds that mimic extracellular matrices. However, traditional two‐dimensional (2D) electrospun nanofibrous mats still have some inherent disadvantages for bone tissue engineering, such as limited cell infiltration and lack of three‐dimensional (3D) structure. The development of 3D electrospun scaffolds with larger pore sizes and porosity provides new perspectives for electrospinning‐based tissue engineering scaffolds. However, there are still some challenges and areas for improvement. In this review, the applications of 3D electrospun nanofibrous scaffolds in the field of bone tissue engineering from its fabrication methods to bio‐functionalization are summarized, with the aim of providing new insights into the design of electrospinning‐based bone tissue engineering scaffolds.

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Endowing iPSC-Derived MSCs with Angiogenic and Keratinogenic Differentiation Potential: A Promising Cell Source for Skin Tissue Engineering

Cited by 14 publications

References 34 publications

Effects of PRP and LyPRP on osteogenic differentiation of MSCs

Effects of PRP and LyPRP on osteogenic differentiation of MSCs

Stem cell‐mediated angiogenesis in skin tissue engineering and wound healing

Three‐dimensional electrospun nanofibrous scaffolds for bone tissue engineering

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