Dissemination of Extended-Spectrumβ-Lactamase- and AmpCβ-Lactamase-ProducingEscherichia coliwithin the Food Distribution System of Ho Chi Minh City, Vietnam

Nguyen, Do Phuc; Nguyen, Thi Anh Dao; Le, Thi Hien; Tran, Nguyen Minh Doan; Ngo, Thanh Phong; Dang, Van Chinh; Kawai, Takao; Kanki, Masashi; Kawahara, Ryuji; Jinnai, Michio; Yonogi, Shinya; Hirai, Yuji; Yamamoto, Yoshimasa; Kumeda, Yuko

doi:10.1155/2016/8182096

Cited by 49 publications

(35 citation statements)

References 34 publications

(44 reference statements)

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“…The prevalence of E. coli in chicken samples 12% was nearly similar with that was reported by Momtaz et al (2012) and Zende et al (2013) who reported that the incidence of E.coli in chicken meat was 11% and 16.67 %, respectively. On the other hand, the present results were lower than those reported by Hyun-jung et al (2015) and Nguyen et al (2016) who reported E.coli presented in chicken meat with percentage 75.9% and 92.7%, respectively. These differences could be attributed to the hygienic measures proceeded in different localities under investigation and health condition of the meat handlers.…”

Section: Discussioncontrasting

confidence: 95%

Detection of Shiga toxin produced by Escherichia coli in poultry and meat in Luxor city using multiplex PCR

Daoud

Karmi

Nasef

et al. 2016

Benha Veterinary Medical Journal

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Shiga toxins were widely spread in the meat especially in minced meat. These toxins have an important significance to human health because it is a major cause of food poisoning. About 150 meat samples purchased from a number of supermarkets and butcher shops in Luxor city were examined for presence of E. coli (50 raw meat samples-50 minced meat samples-50 sample of chicken meat). 62 samples were positive for E.coli spp. 26 isolates were confirmed serologically using O &H specific antisera as E.coli. Incidence of E. coli was in chicken meat 6/50 (12%) and raw meat 11/50 (22%) and minced meat 9/50 (18%). 26 E. coli isolates tested serology using special antisera (O & H) recognize that there are 12 genetic groups They are O26:

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Section: Discussioncontrasting

confidence: 95%

Detection of Shiga toxin produced by Escherichia coli in poultry and meat in Luxor city using multiplex PCR

Daoud

Karmi

Nasef

et al. 2016

Benha Veterinary Medical Journal

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“…A likely explanation to this high level of nonsusceptibility could be the extensive use of antimicrobial in chickens in Vietnam, often without veterinary prescription, as reported in many studies [17–19]. Other studies have reported similar levels of AMR in E. coli from poultry in Vietnam [26, 27]. In contrast, the level of nonsusceptibility to 3GC was very low (3.9%) in indicator isolates, agreeing with the prevalence of 3.1% of resistance reported in 3GC in Vietnam [27].…”

Section: Discussionmentioning

confidence: 97%

Pathogenic potential and the role of clones and plasmids in beta-lactamase-producing E. coli from chicken faeces in Vietnam

et al. 2019

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Background Antimicrobial resistance (AMR) in food-producing animals is a global public health issue. This study investigated AMR and virulence profiles of E. coli isolated from healthy chickens in Vietnam. E. coli were isolated from fecal samples collected in five chicken farms located in the provinces of Hoa Binh, Thai Nguyen and Bac Giang in the North of Vietnam. These isolates were examined by disk diffusion for their AMR, PCR for virulence and AMR genes, pulsed-field gel electrophoresis for relatedness between bla CMY-2 / bla CTX-M –positive isolates, electroporation for transferability of bla CMY-2 or bla CTX-M genes and sequencing for mutations responsible for ciprofloxacin resistance. Results Up to 99% of indicator isolates were multidrug resistant. Resistance to third-generation cephalosporins (3GC) was encoded by both bla CTX-M and bla CMY-2 genes; bla CTX-M genes being of genotypes bla CTX-M-1 , − 14 , − 15 , − 17 , − 57 and − 87 , whereas ciprofloxacin resistance was due to mutations in the gyrA and parC genes. Some isolates originating from farms located in different provinces of Vietnam were found to be closely related, suggesting they may have been disseminated from a same source of contamination. Plasmids may also have played a role in the diffusion of 3GC-resistance as the bla CMY-2 gene was located on plasmids A/C and I1, and the bla CTX-M gene variants were carried by I1, FIB, R and HI1. Plasmids carrying the bla CMY-2 / bla CTX-M genes also co-transferred resistance to other antimicrobials. In addition, isolates potentially capable of infecting humans, of which some produced bla CMY-2 / bla CTX-M , were identified in this study. Conclusions Both clones and plasmids could be involved in the dissemination of 3GC-resistant E. coli within and between chicken farms in Vietnam. These results demonstrate the necessity to monitor AMR and control antimicrobial use in poultry in Vietnam. Electronic supplementary material The online version of this article (10.1186/s12917-019-1849-1) co...

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“…The authors showed that all strains of the Enterobacteriaceae family showed to be AmpC enzyme carriers and showed resistance to at least 6 classes of antimicrobials, including beta-lactams. The presence of these multiresistant multi-resistant strains is a concern for public health and suggests that the use of antimicrobial agents in fish culture is to be strictly controlled 1 .…”

Section: Discussionmentioning

confidence: 99%

“…Enterobacteria are often associated with drug resistance and contamination of fish and shrimp 1 . Food contamination with multidrug-resistant bacteria, particularly extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae, is considered a potential source for the wide dissemination of ESBL-producing bacteria in communities 2 .…”

Section: Introductionmentioning

confidence: 99%

Drug resistance, AmpC-β-lactamase and extended-spectrum β-lactamase-producing Enterobacteriaceae isolated from fish and shrimp

Almeida

Cangussú

Carvalho³

et al. 2017

Rev. Inst. Med. trop. S. Paulo

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The present study aims to detect the production of extended-spectrum beta-lactamases (ESBL) by enterobacteria isolated from samples of fresh shrimp and fish obtained from the retail trade of the city of Sobral, Ceará State, Brazil. All bacterial isolates were submitted to identification and antimicrobial susceptibility testing using aminopenicillin, beta-lactamase inhibitors, carbapenem, 1st, 2nd, 3rd and 4th generation cephalosporins, and monobactam. Three types of beta-lactamases - ESBL, AmpC and KPC - were investigated. 103 strains were identified, and the most frequent species in shrimp and fish samples was Enterobacter cloacae (n = 54). All the strains were resistant to penicillin and more than 50% of the isolates were resistant to ampicillin and cephalothin. Resistance to three 3rd generation cephalosporins (cefotaxime, ceftriaxone and ceftazidime) and one fourth generation cephalosporin (cefepime) was detected in two isolates of E. cloacae from shrimp samples. Phenotypic detection of AmpC was confirmed in seven strains. The ESBL was detected in two strains of E. cloacae from shrimp samples. No strain showed KPC production. These data can be considered alarming, since food (shrimp and fish) may be carriers of enterobacteria resistant to drugs of clinical interest.

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Dissemination of Extended-Spectrumβ-Lactamase- and AmpCβ-Lactamase-ProducingEscherichia coliwithin the Food Distribution System of Ho Chi Minh City, Vietnam

Cited by 49 publications

References 34 publications

Detection of Shiga toxin produced by Escherichia coli in poultry and meat in Luxor city using multiplex PCR

Detection of Shiga toxin produced by Escherichia coli in poultry and meat in Luxor city using multiplex PCR

Pathogenic potential and the role of clones and plasmids in beta-lactamase-producing E. coli from chicken faeces in Vietnam

Drug resistance, AmpC-β-lactamase and extended-spectrum β-lactamase-producing Enterobacteriaceae isolated from fish and shrimp

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