Effects of short‐term severe and long‐term mild STZ‐induced diabetes in urethral tissue of female rats

Abstract

Either long-term mild hyperglycemia or short-term severe hyperglycemia have detrimental impacts on muscle health. They are both involved in the failure to maintain healthy skeletal muscle that may contribute to the development of pelvic floor dysfunctions via different pathways. These results have important implications for monitoring and prevention strategies for improving the quality of life of women with diabetes mellitus and pelvic floor muscle dysfunction. Neurourol. Urodynam. 36:574-579, 2017. © 2016 Wiley Periodicals, Inc.

The influence of hyperglycemia on the remodeling of urethral connective tissue in pregnant rats

“…Female PFD is a common disorder that disables many women with diabetes during pregnancy but its etiology remains unclear [ [22][23][24],9]. Previous translational studies confirmed that a reduction in muscle content by diabetes may negatively affect the structural and functional integrity of the urethra [22][23][24]. The results are expressed as ratios of respective areas in mm 2 .…”

Alterations in the structural characteristics of rectus abdominis muscles caused by diabetes and pregnancy: A comparative study of the rat model and women

“…The consequences of UI persist not only during pregnancy but up to 2 years post-partum and have a negative impact on the quality of life [14,15]. Previously, our research group conducted studies on the urethral tissue obtained from a pregnant Streptozotocin (STZ) rat model [16][17][18][19][20][21]. The hyperglycemia status in these rats is manifested by STZinduced necrosis of the pancreatic β-cells [22].…”

Study protocol to investigate biomolecular muscle profile as predictors of long-term urinary incontinence in women with gestational diabetes mellitus

“…These characteristics differentiated the urethral striated muscles of short-term severe diabetic pregnant rats and long-term mild diabetic pregnant rats from virgin, pregnant and diabetic control groups [20]. The diabetic models were obtained by streptozotocin injection, and according to the dose and duration of the hyperglycemic stimulus, they are classified as short-term severe diabetes or long-term mild diabetes [25,26]. The increase in collagen and severe fibrosis may also affect the contractile properties of the urethra [26,27].…”

“…The diabetic models were obtained by streptozotocin injection, and according to the dose and duration of the hyperglycemic stimulus, they are classified as short-term severe diabetes or long-term mild diabetes [25,26]. The increase in collagen and severe fibrosis may also affect the contractile properties of the urethra [26,27]. The ECM molecular analyses of urethral components showed no difference in type I and type III collagen expression levels in short-term severe diabetic pregnant rats.…”

“…In conclusion, these experimental results demonstrated that both long-term mild hyperglycemia and short-term severe hyperglycemia negatively impacted the muscle health in rats. RAM is subjected to similar structural changes related to pregnant diabetic myopathy [25,26,28] with intramuscular transformation and reorganization in fiber types [30][31][32].…”