Phase-0/phase-I study of dye-loaded lipid nanoparticles for near-infrared fluorescence imaging in healthy dogs

Sayag, D.; Cabon, Quentin; Texier, Isabelle; Navarro, Fabrice; Boisgard, Raphaël; Virieux-Watrelot, Dorothée; Carozzo, Claude; Ponce, Frédérique

doi:10.1016/j.ejpb.2016.01.001

Cited by 23 publications

(20 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Given that ICG is an amphiphilic dye, it can be loaded both inside the lipid core and at the interface of lipid NPs. The pharmacologic and toxicological characteristics of ICG-loaded lipid NPs have been intensively investigated in Beagle dogs 12 . After systematic administration, plasma fluorescence continuously decreased, whereas fluorescence signals mainly occurred in the liver and to a lesser extent in the steroid-rich organs such as adrenal and ovaries, intestines, kidneys, and lymph nodes.…”

Section: In Vivo Biodistribution Of Icg Npsmentioning

confidence: 99%

Indocyanine green-incorporating nanoparticles for cancer theranostics

et al. 2018

View full text Add to dashboard Cite

Indocyanine green (ICG) is a near-infrared dye that has been used in the clinic for retinal angiography, and defining cardiovascular and liver function for over 50 years. Recently, there has been an increasing interest in the incorporation of ICG into nanoparticles (NPs) for cancer theranostic applications. Various types of ICG-incorporated NPs have been developed and strategically functionalised to embrace multiple imaging and therapeutic techniques for cancer diagnosis and treatment. This review systematically summaries the biodistribution of various types of ICG-incorporated NPs for the first time, and discusses the principles, opportunities, limitations, and application of ICG-incorporated NPs for cancer theranostics. We believe that ICG-incorporated NPs would be a promising multifunctional theranostic platform in oncology and facilitate significant advancements in this research-active area.

show abstract

Section: In Vivo Biodistribution Of Icg Npsmentioning

confidence: 99%

Indocyanine green-incorporating nanoparticles for cancer theranostics

et al. 2018

View full text Add to dashboard Cite

show abstract

“…As lipid nanoparticles are considered as carriers without imaging ability, the lowest amount is injected in the body, the best safety is expected (or the undesired effects are limited). Then, the formulation composed of 91 dyes/particle was selected for further preclinical studies (Cabon et al, 2016;Sayag et al, 2016).…”

Section: Applicability Of the Validated Hplc-fld Method: Optimizationmentioning

confidence: 99%

“…Studies in mice have demonstrated that LipImage®815 displays prolonged circulation within the bloodstream and preferential accumulation within tumors; facilitating fluorescent imaging over 22 days after injection (Jacquart et al, 2013). The toxicity and biodistribution of LipImage®815 have been rapidly investigated in clinical trials (Phase 0/I) on healthy beagle dogs (Sayag et al, 2016). Recently, the potential of this agent as an in vivo NIR nanotracer has been evaluated for intraoperative fluorescence imaging in the surgical excision of malignant masses in dogs (Cabon et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Development and validation of an HPLC-fluorescence method for the quantification of IR780-oleyl dye in lipid nanoparticles

Varache

Escudé

Laffont

et al. 2017

International Journal of Pharmaceutics

View full text Add to dashboard Cite

A reversed-phase (RP) high-performance liquid chromatography (HPLC) method for the content determination of IR780-oleyl (IRO) dye in lipid nanoparticles was developed and validated. Chromatographic separation was performed on a RP C18 column with a gradient program of water and acetonitrile both with 0.1% (v/v) TFA, at a flow rate of 1.0mL/min and a total run of 21min. IRO dye detection was made by fluorescence at emission wavelength of 773nm (excitation wavelength: 744nm). According to ICH guidelines, the developed method was shown to be specific, linear in the range 3-8μg/mL (R=0.9998), precise at the intra-day and inter-day levels as reflected by the coefficient of variation (CV≤1.98%) at three different concentrations (4, 6 and 8 μg/mL) and accurate, with recovery rates between 98.2-101.6% and 99.2-100.5%. The detection and quantitation limits were 0.41 and 1.24μg/mL, respectively. Stability studies of sample processing showed that IRO dye was stable after 24h in the autosampler or after three freeze/thaw cycles. Combined with fluorescence measurements, the developed method was successfully applied to optimize the loading capacity of IRO dye in the core of lipid nanoparticles.

show abstract

“…Real-time fluorescence imaging was performed using the compact and portable 2D fluorescence reflectance imaging devices, Fluobeam ® 700 or Fluobeam ® 800, according to the Angiostamp™ labeling. The Fluobeam ® 800 device was used with a dedicated single-use sterile cover bearing an optical window, compatible with NIR fluorescence measurements and with the aseptic conditions of the operating room as described previously by Cabon et al[ 44 , 45 ].…”

Section: Methodsmentioning

confidence: 99%

Fluorescence-guided surgery for cancer patients: a proof of concept study on human xenografts in mice and spontaneous tumors in pets

Mery-Lamarche

Golzio

Guillermet³

et al. 2017

Oncotarget

View full text Add to dashboard Cite

Surgery is often the first treatment option for patients with cancer. Patient survival essentially depends on the completeness of tumor resection. This is a major challenge, particularly in cases of peritoneal carcinomatosis, where tumors are widely disseminated in the large peritoneal cavity. Any development to help surgeons visualize these residual cells would improve the completeness of the surgery. For non-disseminated tumors, imaging could be used to ensure that the tumor margins and the draining lymph nodes are free of tumor deposits.Near-infrared fluorescence imaging has been shown to be one of the most convenient imaging modalities. Our aim was to evaluate the efficacy of a near-infrared fluorescent probe targeting the αvβ3 integrins (Angiostamp™) for intraoperative detection of tumors using the Fluobeam® device.We determined whether different human tumor nodules from various origins could be detected in xenograft mouse models using both cancer cell lines and patient-derived tumor cells. We found that xenografts could be imaged by fluorescent staining irrespective of their integrin expression levels. This suggests imaging of the associated angiogenesis of the tumor and a broader potential utilization of Angiostamp™. We therefore performed a veterinary clinical trial in cats and dogs with local tumors or with spontaneous disseminated peritoneal carcinomatosis. Our results demonstrate that the probe can specifically visualize both breast and ovarian nodules, and suggest that Angiostamp™ is a powerful fluorescent contrast agent that could be used in both human and veterinary clinical trials for intraoperative detection of tumors.

show abstract

Phase-0/phase-I study of dye-loaded lipid nanoparticles for near-infrared fluorescence imaging in healthy dogs

Cited by 23 publications

References 26 publications

Indocyanine green-incorporating nanoparticles for cancer theranostics

Indocyanine green-incorporating nanoparticles for cancer theranostics

Development and validation of an HPLC-fluorescence method for the quantification of IR780-oleyl dye in lipid nanoparticles

Fluorescence-guided surgery for cancer patients: a proof of concept study on human xenografts in mice and spontaneous tumors in pets

Contact Info

Product

Resources

About