Expression and significance of telomerase in the nucleus pulposus tissues of degenerative lumbar discs

Zhang, Xu; Yang, Ming‑Kun; Li, Zhou; Liu, Chuan; Wu, Ji-sheng; Wang, Jie

doi:10.3892/br.2015.516

Cited by 2 publications

(3 citation statements)

References 21 publications

(20 reference statements)

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“…It also has been confirmed that telomerase activity is inhibited or destroyed in the degenerating intervertebral disc cells. Therefore, the cells cannot maintain chromosome balance, leading to continuous shortening of the telomere in the replication process and promoting cell senescence and intervertebral disc degeneration [ 94 - 96 ].…”

Section: The Cause and Effect Chain In Degenerative Disc Diseasementioning

confidence: 99%

Updated Understanding of the Degenerative Disc Diseases - Causes Versus Effects - Treatments, Studies and Hypothesis

Muresanu¹,

Somasundaram

Neganova

et al. 2020

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Background: In this review we survey medical treatments and research strategies, and we discuss why they have failed to cure degenerative disc diseases or even slow down the degenerative process. Objective: We seek to stimulate discussion with respect to changing the medical paradigm associated with treatments and research applied to degenerative disc diseases. Method proposal: We summarize a Biological Transformation therapy for curing chronic inflammations and degenerative disc diseases, as was previously described in the book Biological Transformations controlled by the Mind Volume 1. Preliminary studies: A single-patient case study is presented that documents complete recovery from an advanced lumbar bilateral discopathy and longterm hypertrophic chronic rhinitis by application of the method proposed. Conclusion: Biological transformations controlled by the mind can be applied by men and women in order to improve their quality of life and cure degenerative disc diseases and chronic inflammations illnesses.

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Section: The Cause and Effect Chain In Degenerative Disc Diseasementioning

confidence: 99%

Updated Understanding of the Degenerative Disc Diseases - Causes Versus Effects - Treatments, Studies and Hypothesis

Muresanu¹,

Somasundaram

Neganova

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Telomere attrition is associated with this phenomenon; when the telomere is shortened, it results in senescence and ultimately apoptosis, which further deteriorates the condition of the cells and the IVD ( 8 ). Studies have indicated that diabetes accelerates telomere attrition in type 2 diabetic patients ( 9 , 10 ).…”

Section: Introductionmentioning

confidence: 99%

“…Studies have also demonstrated that telomere attrition and lower telomerase expression is involved in diverse types of diseases, including IVDD. Therefore, telomerase is considered a potential therapeutic target for these diseases ( 8 , 9 ). Telomerase reverse transcriptase (TERT), together with the telomerase RNA component (TERC), is the most important unit of the telomerase complex, though TERT is able to perform its function without the presence of TERC ( 11 ).…”

Section: Introductionmentioning

confidence: 99%

Cycloastragenol and Astragaloside IV activate telomerase and protect nucleus pulposus cells against high glucose‑induced senescence and apoptosis

et al. 2021

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In diabetes-induced intervertebral disc degeneration (Db-IVDD), senescence and apoptosis of nucleus pulposus cells (NPCs) are major contributing factors. Telomere attrition and telomerase downregulation are some of the main reasons for senescence and eventual apoptosis. The derivatives of the Chinese herb Astragalus membranaceus , Cycloastragenol (CAG) and Astragaloside IV (AG-IV), are reportedly effective telomerase activators against telomere shortening; however, their effect in Db-IVDD have not been explored. The present study simultaneously investigated the regulation of these derivatives on senescence, apoptosis, telomeres and telomerase a model of high-glucose (HG)-induced stress using rat primary NPCs. The NPCs were stimulated with HG (50 mM) to evoke HG-induced stress, and the effects of CAG and AG-IV were observed on: i) The expression level of senescence marker p16; ii) β-Gal staining; iii) the expression levels of apoptosis markers cleaved-caspase 3 (c-C3), BAX and Bcl-2; iv) telomerase activation with telomerase reverse transcriptase (TERT) mRNA and protein expression, while telomere length was measured with reverse transcription-quantitative PCR. Cell proliferation was determined using the Cell Counting Kit-8 assay. Results demonstrated an upregulation in the expression levels of p16, c-C3 and BAX, and increased β-Gal staining; while the expression level of Bcl-2 was downregulated in a concentration-dependent manner. Pre-treatment of the NPCs with CAG and AG-IV downregulated the protein expression levels of p16, c-C3 and BAX, and decreased the percentage of β-Gal and FITC staining; while upregulating the Bcl-2 expression. These effects protected the cells from HG stress-induced senescence and apoptosis. HG also downregulated the expression profile of TERT and shortened the telomere length in a glucose concentration-dependent manner. While pretreatment with CAG and AG-IV upregulated TERT expression and ameliorated the telomere attrition. CAG and AG-IV also increased cell proliferation and improved cell morphology in HG conditions. Overall, these findings indicated that CAG and AG-IV suppressed HG stress-induced senescence and apoptosis, in addition to enhancing telomerase activation and lengthening of the Telomere. Therefore, CAG and AG-IV prolonged the replicative capability and longevity of the NPCs and they have the potential to be therapeutic agents in Db-IVDD.

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Expression and significance of telomerase in the nucleus pulposus tissues of degenerative lumbar discs

Cited by 2 publications

References 21 publications

Updated Understanding of the Degenerative Disc Diseases - Causes Versus Effects - Treatments, Studies and Hypothesis

Updated Understanding of the Degenerative Disc Diseases - Causes Versus Effects - Treatments, Studies and Hypothesis

Cycloastragenol and Astragaloside IV activate telomerase and protect nucleus pulposus cells against high glucose‑induced senescence and apoptosis

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