The influence of alternative pathways of respiration that utilize branched‐chain amino acids following water shortage in <i>Arabidopsis</i>

Pires, Marcel Viana; Júnior, Adilson A. Pereira; Medeiros, David B.; Daloso, Danilo de Menezes; Pham, Phuong Anh; Barros, Kallyne A.; Engqvist, Martin K. M.; Florian, Alexandra; Krahnert, Ina; Maurino, Verónica G.; Araújo, Wagner L.; Fernie, Alisdair R.

doi:10.1111/pce.12682

Cited by 144 publications

(121 citation statements)

References 89 publications

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“…Previous studies showed that degradation of branched-chain amino acids and lysine serve as alternative electron donors to maintain respiration during dark-induced senescence through the electron-transfer flavoprotein (ETF) and electron-transfer flavoprotein:ubiquinone oxidoreductase (ETFQO) system in Arabidopsis (16 -23). The loss-of-function mutants of these processes also showed enhanced susceptibility to drought stress (94). Interestingly, the etf and etfqo mutants, which cannot transfer electrons derived from amino acid oxidation to ubiquinone, accumulate aromatic amino acids, including Tyr, besides branched-chain amino acids and lysine (21,22).…”

Section: Physiological and Metabolic Roles Of Tyr Degradation During mentioning

confidence: 99%

TAT1 and TAT2 tyrosine aminotransferases have both distinct and shared functions in tyrosine metabolism and degradation in Arabidopsis thaliana

Wang

Toda

Block

et al. 2019

Journal of Biological Chemistry

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Edited by F. Peter Guengerich Plants produce various L-tyrosine (Tyr)-derived compounds that are critical for plant adaptation and have pharmaceutical or nutritional importance for human health. Tyrosine aminotransferases (TATs) catalyze the reversible reaction between Tyr and 4-hydroxyphenylpyruvate (HPP), representing the entry point in plants for both biosynthesis of various natural products and Tyr degradation in the recycling of energy and nutrients. To better understand the roles of TATs and how Tyr is metabolized in planta, here we characterized single and double loss-of-function mutants of TAT1 (At5g53970) and TAT2 (At5g36160) in the model plant Arabidopsis thaliana. As reported previously, tat1 mutants exhibited elevated and decreased levels of Tyr and tocopherols, respectively. The tat2 mutation alone had no impact on Tyr and tocopherol levels, but a tat1 tat2 double mutant had increased Tyr accumulation and decreased tocopherol levels under highlight stress compared with the tat1 mutant. Relative to WT and the tat2 mutant, the tat1 mutant displayed increased vulnerability to continuous dark treatment, associated with an early drop in respiratory activity and sucrose depletion. During isotope-labeled Tyr feeding in the dark, we observed that the tat1 mutant exhibits much slower 13 C incorporation into tocopherols, fumarate, and other tricarboxylic acid (TCA) cycle intermediates than WT and the tat2 mutant. These results indicate that TAT1 and TAT2 function together in tocopherol biosynthesis, with TAT2 having a lesser role, and that TAT1 plays the major role in Tyr degradation in planta. Our study also highlights the importance of Tyr degradation under carbon starvation conditions during dark-induced senescence in plants.

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Section: Physiological and Metabolic Roles Of Tyr Degradation During mentioning

confidence: 99%

TAT1 and TAT2 tyrosine aminotransferases have both distinct and shared functions in tyrosine metabolism and degradation in Arabidopsis thaliana

Wang

Toda

Block

et al. 2019

Journal of Biological Chemistry

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“…Mitochondrial metabolism was reported to be highly active during DS responses [45]. DS-induced metabolic reprogramming leads to up-regulated concentrations of amino acids and intermediates from TCA cycle, including cis-aconitate, isocitrate, citrate, fumarate, 2-oxoglutarate, succinate, malate, glycolate, putrescine, spermidine, gamma-aminobutyric acid (GABA,) guanidine, fructose, galactose, glucose, maltose, mannose, raffinose, ribose, sucrose, trehalose, dehydroascorbate, alanine (Ala), aspartate (Asp), glutamate (Glt), glutamine (Gln), Ile, Leu, lysine (Lys), methionine (Met), ornithine (Orn), Phe, proline (Pro), serine (Ser), threonine (Thr), Try, and Val, as well as the decreased concentrations of protein, starch and nitrate.…”

Section: The Application Of Metabolomics To Arabidopsis Model-basementioning

confidence: 99%

Metabolomics, a Powerful Tool for Agricultural Research

Tian

Lam

Shui

2016

IJMS

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Metabolomics, which is based mainly on nuclear magnetic resonance (NMR), gas-chromatography (GC) or liquid-chromatography (LC) coupled to mass spectrometry (MS) analytical technologies to systematically acquire the qualitative and quantitative information of low-molecular-mass endogenous metabolites, provides a direct snapshot of the physiological condition in biological samples. As complements to transcriptomics and proteomics, it has played pivotal roles in agricultural and food science research. In this review, we discuss the capacities of NMR, GC/LC-MS in the acquisition of plant metabolome, and address the potential promise and diverse applications of metabolomics, particularly lipidomics, to investigate the responses of Arabidopsis thaliana, a primary plant model for agricultural research, to environmental stressors including heat, freezing, drought, and salinity.

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“…MapMan pathway analysis showed significant enrichment for genes associated with degradation of branchedchain amino acids in WD-repressed genes of the crown (Dataset S3). This alternative respiration pathway has recently been shown to affect drought tolerance in Arabidopsis (19).…”

Section: Suppression Of Crown Root Growth Is a Major Response To Watermentioning

confidence: 99%

Grasses suppress shoot-borne roots to conserve water during drought

Sebastián

Yee

Viana

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

100

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Many important crops are members of the Poaceae family, which develop root systems characterized by a high degree of root initiation from the belowground basal nodes of the shoot, termed the crown. Although this postembryonic shoot-borne root system represents the major conduit for water uptake, little is known about the effect of water availability on its development. Here we demonstrate that in the model C 4 grass Setaria viridis, the crown locally senses water availability and suppresses postemergence crown root growth under a water deficit. This response was observed in field and growth room environments and in all grass species tested. Luminescence-based imaging of root systems grown in soil-like media revealed a shift in root growth from crown-derived to primary root-derived branches, suggesting that primary root-dominated architecture can be induced in S. viridis under certain stress conditions. Crown roots of Zea mays and Setaria italica, domesticated relatives of teosinte and S. viridis, respectively, show reduced sensitivity to water deficit, suggesting that this response might have been influenced by human selection. Enhanced water status of maize mutants lacking crown roots suggests that under a water deficit, stronger suppression of crown roots actually may benefit crop productivity.root development | drought | Poaceae | Setaria | Zea mays

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The influence of alternative pathways of respiration that utilize branched‐chain amino acids following water shortage in Arabidopsis

Cited by 144 publications

References 89 publications

TAT1 and TAT2 tyrosine aminotransferases have both distinct and shared functions in tyrosine metabolism and degradation in Arabidopsis thaliana

TAT1 and TAT2 tyrosine aminotransferases have both distinct and shared functions in tyrosine metabolism and degradation in Arabidopsis thaliana

Metabolomics, a Powerful Tool for Agricultural Research

Grasses suppress shoot-borne roots to conserve water during drought

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