Search for endophytic diazotrophs in barley seeds

Zawoznik, Myriam Sara; Vázquez, Susana; Herrera, Silvana Marcela Díaz; Groppa, María Daniela

doi:10.1590/s1517-83822014000200033

Cited by 17 publications

(12 citation statements)

References 24 publications

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“…From all identified tef seed endophytic bacterial strains, majority of them are belonged to genus Pseudomonas, some of the endophytes belonged to genus Pantoae and Enterobacter, whereas few of them belonged to genus Bacillus, Serratia and Razobium. The present study result was correlated with the previous work of Zawoznik [17], he reported that eight endophytic bacteria isolates assigned as Pseudomonas, Azospirillum, and Bacillus genera were isolated from barley seeds under selective pressure for nitrogen-fixing bacteria. For a biotic environmental stress condition, salinity is one of the major factors that adversely affect crops production and productivity in the world.…”

Section: Discussionsupporting

confidence: 91%

“…Johnston-Monje and Raizada [16] reported that most of the bacterial isolates from seeds of different maize varieties were able to solubilize phosphorus and fix nitrogen. In addition, Zawoznik [17] reported that eight endophytic bacteria designated to Pseudomonas, Azospirillum, and Bacillus genera were isolated from barley seeds under selective pressure for nitrogen-fixing bacteria.…”

Section: Introduction Background and Justificationmentioning

confidence: 99%

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Characterization and Identification of Tef (Eragrostis tef) Seed Endophytic Bacterial Species and Evaluate their Effect on Plant Growth Promotion

Tsegaye¹,

Assefa²,

Tefera³

et al. 2018

J Clin Med Sci

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In the present day there is no report on characterization and identification of bacterial endophytes isolated from tef (Eragrostis tef) seeds. This study was conducted to screen, identify, and characterize bacterial endophytes isolated from tef seeds germplasm repository and determine if the bacterial provided plant growth promotion. Nine endophytic bacterial species were identified from 83 tef seed accessions using a Biolog microbial identification system, which utilize 95 different carbon sources. Eight of the identified bacterial species could produce amylase, seven of the species could solubilize phosphate and six of the bacteria could degrade cellulose. All the bacteria were shown to enhance growth of wheat (Triticum aestivum) under laboratory condition. Pseudomonas stutzeri, Rhizobium radiobacter, Bacillus butanolivorans, Pseudomonas putida biotype B, Enterobacter cowanii, Pantoea dispersa, Enterobacter cloacae ss dissolvens, Serratia ficaria and Pantoea agglomerans significantly increased mean root dry mass of inoculated Triticum aestivum up to 9.8%, 9.3%, 8.1%, 7.9%, 7.7%, 7.5%, 7%, 6.9% and 5.5% respectively and increase the mean shoot dry mass of Triticum aestivum up to 29%, 25%, 23%, 26%, 23%, 20%, 22% and 19% respectively. In addition, several seed endophytic bacterial species exhibited tolerance to salinity up to 6% and only Bacillus butanolivorans tolerated salinity up to 15% and temperature up to 60°C, this suggested the potential for possible application under stressed environmental conditions and possible utility as bioinoculant for maintaining sustainable agricultural production and productivity without affecting human health.

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Section: Discussionsupporting

confidence: 91%

Section: Introduction Background and Justificationmentioning

confidence: 99%

Characterization and Identification of Tef (Eragrostis tef) Seed Endophytic Bacterial Species and Evaluate their Effect on Plant Growth Promotion

Tsegaye¹,

Assefa²,

Tefera³

et al. 2018

J Clin Med Sci

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“…Species participating in our biofertilizer consortium, such as Serratia liquefaciens (Kalbe et al 1996) and Stenotrophomonas maltophilia (Berg et al 1996), are known as beneficial rhizobacteria. Pseudomonas putida (Zawoznik et al 2014) and most species of Serratia (Kalbe et al 1996) secrete IAA, which can directly promote root growth. Several recent studies have demonstrated also beneficial effects for plants, shown by quorum sensing molecules, in particular the N-acyl homoserine lactone group produced by different Gram-negative bacteria (reviewed in Schikora et al 2016) such as S. liquefaciens and P. putida (Schuhegger et al 2006).…”

Section: Resultsmentioning

confidence: 99%

Characteristics of an endophytic microbial consortium and its impact on rhizosphere microbiota of barley

2018

EEB

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Biological fertilizers are being developed on the basis of plant growth promoting microorganisms isolated from the plant rhizosphere, phyllosphere and endosphere. The aim of this study was to determine influence of a consortium of plant endophytes on rhizosphere microbiota in a greenhouse vegetation experiment with barley. Fifty-five bacterial and fungal strains were isolated from leaves, stems and roots of oilseed rape and barley. Twentty five of them did not inhibit seed germination. Most of them belonged to the genera Serratia, Enterobacter, Brevibacillus and Pseudomonas. Individual isolates demonstrated phosphate solubilization ability, nitrification potential and urease activity. Impact of the multi-strain microbial consortium on cultivable microbiota of rhizosphere was estimated in a 19-day greenhouse experiment with barley. Concentration of phosphate solubilizing and cellulolytic microorganisms in the rhizosphere was below the detection limit. The consortium increased the total number of bacterial colony-forming units but did not affect the number of actinobacterial and fungal colony-forming units. Interactions between species in the multi-strain consortium and between introduced and indigenous populations are discussed.

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“…The seed microbiome is increasingly attracting interest, and has been the subject of several recent reviews [ 7 , 41 , 42 , 43 ]. Bacteria have been detected in surface-sterilized seeds of various species, including crop plants like alfalfa [ 44 ], rice [ 25 , 45 , 46 , 47 , 48 , 49 ], maize [ 50 , 51 ], tobacco [ 52 ], coffee [ 53 ], quinoa [ 54 ], common bean [ 55 ], grapevine [ 56 ], barley [ 57 ], and pumpkin [ 58 ], but also in wild plants like the giant cardon cactus ( Pachycereus pringlei ) [ 59 ], annual ryegrass ( Lolium rigidum ) [ 60 ], various species of eucalyptus ( Eucalyptus spp.) [ 61 ], Norway spruce ( Picea abies ) [ 62 ], and the South American tree Anadenanthera colubrina [ 63 ].…”

Section: Vertical Transmissionmentioning

confidence: 99%

Transmission of Bacterial Endophytes

2017

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Plants are hosts to complex communities of endophytic bacteria that colonize the interior of both below- and aboveground tissues. Bacteria living inside plant tissues as endophytes can be horizontally acquired from the environment with each new generation, or vertically transmitted from generation to generation via seed. A better understanding of bacterial endophyte transmission routes and modes will benefit studies of plant–endophyte interactions in both agricultural and natural ecosystems. In this review, we provide an overview of the transmission routes that bacteria can take to colonize plants, including vertically via seeds and pollen, and horizontally via soil, atmosphere, and insects. We discuss both well-documented and understudied transmission routes, and identify gaps in our knowledge on how bacteria reach the inside of plants. Where little knowledge is available on endophytes, we draw from studies on bacterial plant pathogens to discuss potential transmission routes. Colonization of roots from soil is the best studied transmission route, and probably the most important, although more studies of transmission to aerial parts and stomatal colonization are needed, as are studies that conclusively confirm vertical transfer. While vertical transfer of bacterial endophytes likely occurs, obligate and strictly vertically transferred symbioses with bacteria are probably unusual in plants. Instead, plants appear to benefit from the ability to respond to a changing environment by acquiring its endophytic microbiome anew with each generation, and over the lifetime of individuals.

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Search for endophytic diazotrophs in barley seeds

Cited by 17 publications

References 24 publications

Characterization and Identification of Tef (Eragrostis tef) Seed Endophytic Bacterial Species and Evaluate their Effect on Plant Growth Promotion

Characterization and Identification of Tef (Eragrostis tef) Seed Endophytic Bacterial Species and Evaluate their Effect on Plant Growth Promotion

Characteristics of an endophytic microbial consortium and its impact on rhizosphere microbiota of barley

Transmission of Bacterial Endophytes

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