Electrochemical immunosensor for detection of topoisomerase based on graphene–gold nanocomposites

Zhong, Guang‐Xian; Wang, Peng; Fu, Fei-Huan; Weng, Shaohuang; Chen, Wei; Li, Shaoguang; Liu, Ailin; Wu, Zhaoyang; Zhu, Xiaoyang; Lin, Xinhua; Lin, Jianhua; Xia, Xing‐Hua

doi:10.1016/j.talanta.2014.01.037

Cited by 13 publications

(6 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Due to the existence of -SH on graphene surface, oxidase can directly adhere on the film by the interaction produced by the -S-S-from protein and -SH from graphene. [15] One hour adsorption was required to make the oxidase strongly and enough combination on film surface (Fig. 1).…”

Section: Construction Of Glucose Biosensormentioning

confidence: 99%

“…Gold is widely applied as an electrode modifier with excellent catalytic function, especially for its new innovations of nanostructure, such as nanoscaled flower-like, urchin-like, aloe-like and pinecone-like morphologies [12][13][14][15]. However, its high catalytic potential can induce a synchronously oxidation of co-existed substance which may produce an inaccurate factor.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

In-situ fabrication of well-distributed gold nanocubes on thiol graphene as a third-generation biosensor for ultrasensitive glucose detection

Chu

Liu

et al. 2015

Electrochimica Acta

View full text Add to dashboard Cite

Section: Construction Of Glucose Biosensormentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In-situ fabrication of well-distributed gold nanocubes on thiol graphene as a third-generation biosensor for ultrasensitive glucose detection

Chu

Liu

et al. 2015

Electrochimica Acta

View full text Add to dashboard Cite

“…Gold nanostructures (AuNSs) are the most popular substrate for label-free immunosensors due to their highly electroactive surfaces, their strong adsorption ability for proteins and their ease of surface modification with thiolate molecules. AuNS deposition can be fabricated using template [13] or templateless electrodeposition [14,15]. Furthermore, the oriented immobilization of antibodies is another important strategy for improving the sensing properties of immunosensors.…”

Section: Introductionmentioning

confidence: 99%

Label-Free Impedimetric Immunosensors Modulated by Protein A/Bovine Serum Albumin Layer for Ultrasensitive Detection of Salbutamol

Lin

Huang

et al. 2020

Sensors

View full text Add to dashboard Cite

The sensing properties of immunosensors are determined not only by the amount of immobilized antibodies but also by the number of effective antigen-binding sites of the immobilized antibody. Protein A (PA) exhibits a high degree of affinity with the Fc part of IgG antibody to feasibly produce oriented antibody immobilization. This work proposes a simple method to control the PA surface density on gold nanostructure (AuNS)-deposited screen-printed carbon electrodes (SPCEs) by mixing concentration-varied PA and bovine serum albumin (BSA), and to explore the effect of PA density on the affinity attachment of anti-salbutamol (SAL) antibodies by electrochemical impedance spectroscopy. A concentration of 100 μg/mL PA and 100 μg/mL BSA can obtain a saturated coverage on the 3-mercaptoproponic acid (MPA)/AuNS/SPCEs and exhibit a 50% PA density to adsorb the amount of anti-SAL, more than other concentration-varied PA/BSA-modified electrodes. Compared with the randomly immobilized anti-SAL/MPA/AuNS/SPCEs and the anti-SAL/PA(100 μg/mL):BSA(0 μg/mL)/MPA/AuNS/SPCE, the anti-SAL/PA(100 μg/mL): BSA(100 μg/mL)/MPA/AuNS/SPCE-based immunosensors have better sensing properties for SAL detection, with an extremely low detection limit of 0.2 fg/mL and high reproducibility (<2.5% relative standard deviation). The mixture of PA(100 μg/mL):BSA(100 μg/mL) for the modification of AuNS/SPCEs has great promise for forming an optimal protein layer for the oriented adsorption of IgG antibodies to construct ultrasensitive SAL immunosensors.

show abstract

“…The effective immobilization of biomolecules is a key point in fabrication of immunosensors. Furthermore, sensitivity of an electrochemical immunosensor for antigen detection at very low concentration range can be increased by increasing the loading amount of antibodies as well as controlling the orientation of immobilized antibodies on electrode surfaces 16, 17, therefore, there is growing interest in developing new advanced materials and designing novel biosensors with controlled features on a nanometer scale. Carbon nanomaterials, such as multiwalled carbon nanotubes (MWCNTs) have been widely used in fabrication of biosensors because of chemical inertness, low residual current, excellent conductivity, wide potential window, and electrocatalytic activity to a variety of redox reactions 18, 19.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive Electrochemical Immunosensor for Detection of Tumor Necrosis Factor‐α Based on Functionalized MWCNT‐Gold Nanoparticle/Ionic Liquid Nanocomposite

2015

View full text Add to dashboard Cite

A novel and highly sensitive electrochemical immunosensor was developed for the detection of protein biomarker tumor necrosis factor‐alpha (TNF‐α) based on immobilization of TNF‐α‐antibody (anti‐TNF‐α) onto robust nanocomposite containing gold nanoparticles (AuNP), multiwalled carbon nanotubes (MWCNTs) and ionic liquid (1‐buthyl‐3‐methylimidazolium bis (trifluoromethyl sulfonyl)imide). Functionalized MWCNT‐gold nanoparticle was produced by one‐step synthesis based on the direct redox reaction. The electrochemical properties of nanocomposite were characterized by electrochemical impedance spectroscopy and cyclic voltammetry. The anti‐TNF‐α was immobilized or entrapped in the nanocomposite and used in a sandwich type complex immunoassay with anti‐TNF‐α labeled with horseradish peroxidase as secondary antibody. Under optimum conditions, the immunosensor could detect TNF‐α in a linear range from 6.0 to 100 pg mL−1 with a low detection limit of 2.0 pg mL−1. The simple fabrication method, high sensitivity, good reproducibility, stability, as well as acceptable accuracy for TNF‐α detection in human serum samples are the main advantages of this immunosensor, which might have broad applications in protein diagnostics and bioassay.

show abstract

Electrochemical immunosensor for detection of topoisomerase based on graphene–gold nanocomposites

Cited by 13 publications

References 43 publications

In-situ fabrication of well-distributed gold nanocubes on thiol graphene as a third-generation biosensor for ultrasensitive glucose detection

In-situ fabrication of well-distributed gold nanocubes on thiol graphene as a third-generation biosensor for ultrasensitive glucose detection

Label-Free Impedimetric Immunosensors Modulated by Protein A/Bovine Serum Albumin Layer for Ultrasensitive Detection of Salbutamol

Ultrasensitive Electrochemical Immunosensor for Detection of Tumor Necrosis Factor‐α Based on Functionalized MWCNT‐Gold Nanoparticle/Ionic Liquid Nanocomposite

Contact Info

Product

Resources

About