Detection of Helicobacter pylori in adenoid tissue by real-time polymerase chain reaction

Kaymakçı, Mustafa; Aydın, Mehtap; Yazıcı, Selçuk; Sağır, Özlem; Gür, Özer Erdem; Sayan, Murat

doi:10.5606/kbbihtisas.2014.28445

Cited by 4 publications

(2 citation statements)

References 28 publications

(33 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since PCR never indicates the presence of active bacteria in the tissue, it may be argued that they were returned from the stomach to the adenotonsillar tissue (5,16). Similar to our study, H. pylori was detected in the tonsillar and adenoid tissue of children with chronic adenotonsillitis by RUT and nested-PCR (21,(25)(26)(27). The PCR analysis in Bulut et al's ( 16) study showed that 24.6% were positive for H. pylori.…”

Section: Discussionsupporting

confidence: 82%

“…Chronic sinusitis and ear infection may be infected in excess of the adenoid tissue and act as a reservoir for bacteria (15). Gastroesophageal reflux disease (GERD) is considered the cause of ATH (21). In this study, only 17.65% of the 17 patients with GERD history had H. pylori infection in their adenotonsillar tissue, while the prevalence of H. pylori colonization in those with no GERD history was 18.55%.…”

Section: Discussionmentioning

confidence: 70%

See 1 more Smart Citation

Helicobacter pylori Colonization in Patients with Adenotonsillar Hypertrophy: Study on Prevalence and Clinical Characteristics of Colonized Patients and Possible Association with Complications

Pourmousa

Varshoi

Nikkhah

et al. 2020

Arch Pediatr Infect Dis

View full text Add to dashboard Cite

Background: Adenotonsillar hypertrophy (ATH) is a common problem in children that causes the obstruction of airways and other relevant complications. Helicobacter pylori (H. pylori) is considered one of the microbial causes of ATH development. Objectives: The study aimed to determine the H. pylori colonization in patients with adenotonsillar hypertrophy and assess the prevalence and clinical characteristics of colonized patients and possible association with complications. Methods: This study was carried out on 114 children with ATH referring to the Bouali Hospital of Sari from December 2017 to December 2018. Adenotonsillar samples were prepared from the right and left tonsils and adenoid of each patient. The Rapid Urease test (RUT) and nested-PCR were performed on the samples. Data analysis was performed with SPSS version 20 software. Results:The participants included 52 females and 62 males with a mean age of 7.19 ± 3.08 years. The RUT was positive for 59 participants: 26 in right tonsil, 31 in left tonsil, and two in adenoid samples. Helicobacter pylori was detected in the adenotonsillar tissue of 95 patients using nested-PCR. No significant association was found between the PCR results and gender (P = 0.123). Conclusions: This study approved the presence of H. pylori in the adenotonsillar tissue of children with ATH and highlighted the concept that the pharynx could be an extra gastric reservoir of H. pylori. However, we failed to prove an association between H. pylori adenotonsillar colonization with ATH and acute otitis media.

show abstract

Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 70%

Helicobacter pylori Colonization in Patients with Adenotonsillar Hypertrophy: Study on Prevalence and Clinical Characteristics of Colonized Patients and Possible Association with Complications

Pourmousa

Varshoi

Nikkhah

et al. 2020

Arch Pediatr Infect Dis

View full text Add to dashboard Cite

show abstract

Clinical characteristics of obstructive sleep apnea versus infectious adenotonsillar hyperplasia in children

Johnston

McLaren

Mahadevan

et al. 2019

International Journal of Pediatric Otorhinolaryngology

View full text Add to dashboard Cite

Adenotonsillar microbiome: an update

Johnston

Douglas

2018

Postgraduate Medical Journal

View full text Add to dashboard Cite

Pathogenic bacteria associated with the adenoids and tonsils cause much morbidity in the paediatric population. Hyperplasia of the adenoids is associated with otitis media with effusion and hyperplasia of the palatine tonsils is associated with both recurrent tonsillitis and obstructive sleep apnoea. Most current knowledge of the microbiology of the upper airways has been derived from culture-based studies, which usually reflect only a small fraction of the bacteria present on the mucosal surface. Culture-independent molecular surveys based on 16S ribosomal RNA sequencing are now being employed to determine the microbiota on the surface and within the tissue of adenoids and palatine tonsils. This review describes the new techniques applied in determining the microbiome and summarises the results of studies employing these techniques.

show abstract

Detection of Helicobacter pylori in adenoid tissue by real-time polymerase chain reaction

Abstract: In this study the presence of H. pylori in patients with adenoid hypertrophy has been demonstrated using PCR method. To be able to support the hypothesis that H. pylori has a place in etiology of adenoid hypertrophy, multicenter studies are warranted.

Cited by 4 publications

References 28 publications

Helicobacter pylori Colonization in Patients with Adenotonsillar Hypertrophy: Study on Prevalence and Clinical Characteristics of Colonized Patients and Possible Association with Complications

Helicobacter pylori Colonization in Patients with Adenotonsillar Hypertrophy: Study on Prevalence and Clinical Characteristics of Colonized Patients and Possible Association with Complications

Clinical characteristics of obstructive sleep apnea versus infectious adenotonsillar hyperplasia in children

Adenotonsillar microbiome: an update

Contact Info

Product

Resources

About