Photodynamic therapy has antifungal effect and reduces inflammatory signals in Candida albicans-induced murine vaginitis

Machado-de-Sena, Rosa Maria; Corrêa, Luciana; Kato, Ilka Tiemy; Prates, Renato Araújo; Senna, André Machado de; Santos, Carla C.; Picanço, D. A.; Ribeiro, Martha S.

doi:10.1016/j.pdpdt.2014.03.013

Cited by 35 publications

(21 citation statements)

References 27 publications

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“…aPDT with TMP-1363 resulted in a 50-fold reduction of C. albicans CFU/ear compared to untreated controls, and the infected ears subjected to aPDT completely healed over time without any residual damage to the pinna. Machado-de-Sena et al (2014) evaluated the efficacy of aPDT for treatment of C. albicans vaginal infection using MB and red light. Mice were inoculated intravaginally with C. albicans , and then were treated with aPDT 5 days later using MB and red laser.…”

Section: In Vivo Studiesmentioning

confidence: 99%

Antimicrobial photodynamic therapy: an effective alternative approach to control fungal infections

et al. 2015

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Skin mycoses are caused mainly by dermatophytes, which are fungal species that primarily infect areas rich in keratin such as hair, nails, and skin. Significantly, there are increasing rates of antimicrobial resistance among dermatophytes, especially for Trichophyton rubrum, the most frequent etiologic agent worldwide. Hence, investigators have been developing new therapeutic approaches, including photodynamic treatment. Photodynamic therapy (PDT) utilizes a photosensitive substance activated by a light source of a specific wavelength. The photoactivation induces cascades of photochemicals and photobiological events that cause irreversible changes in the exposed cells. Although photodynamic approaches are well established experimentally for the treatment of certain cutaneous infections, there is limited information about its mechanism of action for specific pathogens as well as the risks to healthy tissues. In this work, we have conducted a comprehensive review of the current knowledge of PDT as it specifically applies to fungal diseases. The data to date suggests that photodynamic treatment approaches hold great promise for combating certain fungal pathogens, particularly dermatophytes.

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Section: In Vivo Studiesmentioning

confidence: 99%

Antimicrobial photodynamic therapy: an effective alternative approach to control fungal infections

et al. 2015

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“…Estrus was induced to provide persistent vaginitis, by the weekly subcutaneous injection of 0,1 mg/mL estradiol valerate. Vaginal inoculation of C. albicans was performed 3 days after pseudo estrus and then aPDT was performed 5 days following fungal inoculation [1,5,10].…”

Section: Methodsmentioning

confidence: 99%

“…Fungal suspensions from vaginal wash were serially diluted in PBS to give dilutions of 10 -1 to 10 -3 times the original concentration. Ten microliter aliquots of each group were streaked onto Sabouraud chloramphenicol agar plates in triplicates and incubated at 37ºC overnight for colonies count and transformed to colony for units (CFU) per mL [5,12]. The colony number was divided by the average of control counts on each experiment and data was presented as survival fraction.…”

Section: Microbiological Analysis and Statisticsmentioning

confidence: 99%

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Photodynamic therapy as a new approach in vulvovaginal candidiasis in murine model

et al. 2015

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Vulvovaginal candidiasis is a common cause of vaginal infections. This study investigates the efficiency of antimicrobial photodynamic therapy (aPDT) against yeast cells in mice. Methylene blue (MB), malachite green (MG), and a special designed protoporphirin (PpNetNI) were used as photosensitizers. Female BALB-c mice were infected with Candida albicans ATCC 90028. PDT was applied with two different light sources, intravaginal and transabdominal. Vaginal washes were performed and cultivated for microbial quantification. Antimicrobial PDT was able to decrease microbial content with MB and PpNetNI (p<0.05), it was not effective, however, with MG photosensitizer. The results of this study demonstrate that aPDT may be a viable alternative treatment for vaginal candidiasis.

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“…Vaginitis is a common gynecological problem, which is induced by inflammation or infection of the vagina (1). The most common symptoms of vaginitis are itching, burning and discharge.…”

Section: Introductionmentioning

confidence: 99%

Effects of siRNA-mediated suppression of HPV-11 L1 expression on the proliferation and apoptosis of vaginal epithelial cells

Zeng

Yang

Wang

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. The aim of the present study was to investigate the effects of human papillomavirus (HPV) infection on the gynecological disease of vaginitis and to demonstrate how the small interfering RNA (siRNA) method may be used for HPV prevention in the clinic. Human vaginal epithelial cells were transfected with HPV-11 L1 expression vector and siRNA-HPV-11 L1 vectors and a control group was transfected with scrambled siRNA. Cell proliferation in each group was analyzed using the MTT assay and the expression of apoptosis-associated proteins was measured by western blot analysis. Compared with the control group, HPV-11 L1 mRNA and protein levels were significantly increased following transfection with the HPV-11 L1 expression vector in cells (P<0.05), but this result was significantly reversed by silencing of HPV-11 L1 (P<0.05). In addition, cell proliferation in the HPV-11 group was lower than that in the control group; however, cell proliferation was significantly increased in cells transfected with silenced L1 compared with that in the control group (P<0.05). Furthermore, silencing of HPV-11 L1 significantly decreased caspase-3 and caspase-9 expressions in cells, whereas the expression was increased in the HPV-11 L1 group (P<0.05). The present study suggested that siRNA-mediated silencing of HPV-11 L1 may have potential therapeutic applications for treating gynecological diseases associated with HPV-11 infection.

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Photodynamic therapy has antifungal effect and reduces inflammatory signals in Candida albicans-induced murine vaginitis

Cited by 35 publications

References 27 publications

Antimicrobial photodynamic therapy: an effective alternative approach to control fungal infections

Antimicrobial photodynamic therapy: an effective alternative approach to control fungal infections

Photodynamic therapy as a new approach in vulvovaginal candidiasis in murine model

Effects of siRNA-mediated suppression of HPV-11 L1 expression on the proliferation and apoptosis of vaginal epithelial cells

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