An effective approach for energy conservation in wireless sensor networks is scheduling sleep intervals for extraneous nodes, while the remaining nodes stay active to provide continuous service. For the sensor network to operate successfully, the active nodes must maintain both sensing coverage and network connectivity. Furthermore, the network must be able to configure itself to any feasible degrees of coverage and connectivity in order to support different applications and environments with diverse requirements. This paper presents the design and analysis of novel protocols that can dynamically configure a network to achieve guaranteed degrees of coverage and connectivity. This work... Read complete abstract on page 2.Read complete abstract on page 2.Complete Abstract: Complete Abstract:An effective approach for energy conservation in wireless sensor networks is scheduling sleep intervals for extraneous nodes, while the remaining nodes stay active to provide continuous service. For the sensor network to operate successfully, the active nodes must maintain both sensing coverage and network connectivity. Furthermore, the network must be able to configure itself to any feasible degrees of coverage and connectivity in order to support different applications and environments with diverse requirements. This paper presents the design and analysis of novel protocols that can dynamically configure a network to achieve guaranteed degrees of coverage and connectivity. This work differs from existing connectivity or coverage maintenance protocols in several key ways: 1) We present a Coverage Configuration Protocol (CCP) that can provide different degrees of coverage requested by ap-plications. This flexibility allows the network to self-configure for a wide range of applications and (possibly dynamic) environments. 2) We provide a geometric analysis of the relationship between coverage and connectivity. This analysis yields key insights for treating coverage and connectivity in a unified framework: this is in sharp contrast to several existing approaches that address the two problems in isolation. 3) Finally, we integrate CCP with the SPAN protocol from MIT to provide both coverage and connectivity guarantees. We demonstrate the capability of our protocols to provide guaranteed feasible coverage and connectivity configurations, through both geometric analysis and extensive simulationsAbstract: An effective approach for energy conservation in wireless sensor networks is scheduling sleep intervals for extraneous nodes, while the remaining nodes stay active to provide continuous service. For the sensor network to operate successfully, the active nodes must maintain both sensing coverage and network connectivity. Furthermore, the network must be able to configure itself to any feasible degrees of coverage and connectivity in order to support different applications and environments with diverse requirements. This paper presents the design and analysis of novel protocols that can dynamically configure a network to achieve guaran...

An effective approach for energy conservation in wireless sensor networks is scheduling sleep intervals for extraneous nodes, while the remaining nodes stay active to provide continuous service. For the sensor network to operate successfully, the active nodes must maintain both sensing coverage and network connectivity. Furthermore, the network must be able to configure itself to any feasible degrees of coverage and connectivity in order to support different applications and environments with diverse requirements. This paper presents the design and analysis of novel protocols that can dynamically configure a network to achieve guaranteed degrees of coverage and connectivity. This work differs from existing connectivity or coverage maintenance protocols in several key ways: 1) We present a Coverage Configuration Protocol (CCP) that can provide different degrees of coverage requested by applications. This flexibility allows the network to self-configure for a wide range of applications and (possibly dynamic) environments. 2) We provide a geometric analysis of the relationship between coverage and connectivity. This analysis yields key insights for treating coverage and connectivity in a unified framework: this is in sharp contrast to several existing approaches that address the two problems in isolation. 3) Finally, we integrate CCP with the SPAN protocol from MIT to provide both coverage and connectivity guarantees. We demonstrate the capability of our protocols to provide guaranteed feasible coverage and connectivity configurations, through both geometric analysis and extensive simulations Abstract: An effective approach for energy conservation in wireless sensor networks is scheduling sleep intervals for extraneous nodes, while the remaining nodes stay active to provide continuous service. For the sensor network to operate successfully, the active nodes must maintain both sensing coverage and network connectivity. Furthermore, the network must be able to configure itself to any feasible degrees of coverage and connectivity in order to support different applications and environments with diverse requirements. This paper presents the design and analysis of novel protocols that can dynamically configure a network to achieve guaranteed degrees of coverage and connectivity. This work differs from existing connectivity or coverage maintenance protocols in several key ways: 1) We present a Coverage Configuration Protocol (CCP) that can provide different degrees of coverage requested by applications. This flexibility allows the network to self-configure for a wide range of applications and (possibly dynamic) environments. 2) We provide a geometric analysis of the relationship between coverage and connectivity. This analysis yields key insights for treating coverage and connectivity in a unified framework: this is in sharp contrast to several existing approaches that address the two problems in isolation. 3) Finally, we integrate CCP with the SPAN protocol from MIT to provide both coverage and connectivity guarantees. We...

An effective approach for energy conservation in wireless sensor networks is scheduling sleep intervals for extraneous nodes while the remaining nodes stay active to provide continuous service. For the sensor network to operate successfully, the active nodes must maintain both sensing coverage and network connectivity. Furthermore, the network must be able to configure itself to any feasible degree of coverage and connectivity in order to support different applications and environments with diverse requirements. This article presents the design and analysis of novel protocols that can dynamically configure a network to achieve guaranteed degrees of coverage and connectivity. This work differs from existing connectivity or coverage maintenance protocols in several key ways. (1) We present a Coverage Configuration Protocol (CCP) that can provide different degrees of coverage requested by applications. This flexibility allows the network to self-configure for a wide range of applications and (possibly dynamic) environments. (2) We provide a geometric analysis of the relationship between coverage and connectivity. This analysis yields key insights for treating coverage and connectivity within a unified framework; in sharp contrast to several existing approaches that address the two problems in isolation. (3) We integrate CCP with SPAN to provide both coverage and connectivity guarantees. (4) We propose a probabilistic coverage model and extend CCP to provide probabilistic coverage guarantees. We demonstrate the capability of our protocols to provide guaranteed coverage and connectivity configurations through both geometric analysis and extensive simulations.

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The rational design of theranostic nanoparticles exhibiting synergistic turn-on of therapeutic potency and enhanced diagnostic imaging in response to tumor milieu is critical for efficient personalized cancer chemotherapy. We herein fabricate self-reporting theranostic drug nanocarriers based on hyperbranched polyprodrug amphiphiles (hPAs) consisting of hyperbranched cores conjugated with reduction-activatable camptothecin prodrugs and magnetic resonance (MR) imaging contrast agent (Gd complex), and hydrophilic coronas functionalized with guanidine residues. Upon cellular internalization, reductive milieu-actuated release of anticancer drug in the active form, activation of therapeutic efficacy (>70-fold enhancement in cytotoxicity), and turn-on of MR imaging (∼9.6-fold increase in T1 relaxivity) were simultaneously achieved in the simulated cytosol milieu. In addition, guanidine-decorated hPAs exhibited extended blood circulation with a half-life up to ∼9.8 h and excellent tumor cell penetration potency. The hyperbranched chain topology thus provides a novel theranostic polyprodrug platform for synergistic imaging/chemotherapy and enhanced tumor uptake.

Twist is a critical epithelial-mesenchymal transition (EMT)-inducing transcription factor that increases expression of vimentin. How Twist1 regulates this expression remains unclear. Here, we report that Twist1 regulates Cullin2 (Cul2) circular RNA to increase expression of vimentin in EMT. Twist1 bound the Cul2 promoter to activate its transcription and to selectively promote expression of Cul2 circular RNA (circ-10720), but not mRNA. circ-10720 positively correlated with Twist1, tumor malignance, and poor prognosis in hepatocellular carcinoma (HCC). Twist1 promoted vimentin expression by increasing levels of circ-10720, which can absorb miRNAs that target vimentin. circ-10720 knockdown counteracted the tumor-promoting activity of Twist1 and in patient-derived xenograft and diethylnitrosamine-induced TetOn-Twist1 transgenic mouse HCC models. These data unveil a mechanism by which Twist1 regulates vimentin during EMT. They also provide potential therapeutic targets for HCC treatment and provide new insight for circular RNA (circRNA)-based diagnostic and therapeutic strategies. A circRNA-based mechanism drives Twist1-mediated regulation of vimentin during EMT and provides potential therapeutic targets for treatment of HCC. http://cancerres.aacrjournals.org/content/canres/78/15/4150/F1.large.jpg .

We report on the fabrication of photochromic polymersomes exhibiting photoswitchable and reversible bilayer permeability from newly designed poly(ethylene oxide)-b-PSPA (PEO-b-PSPA) diblock copolymers, where SPA is spiropyran (SP)-based monomer containing a unique carbamate linkage. Upon self-assembling into polymersomes, SP moieties within vesicle bilayers undergo reversible phototriggered isomerization between hydrophobic spiropyran (SP, λ2 > 450 nm irradiation) and zwitterionic merocyanine (MC, λ1 < 420 nm irradiation) states. For both SP and MC polymersomes, their microstructures are stabilized by multiple cooperative noncovalent interactions including hydrophobic, hydrogen bonding, π-π stacking, and paired electrostatic (zwitterionic) interactions, with the latter two types being exclusive for MC polymersomes. Control experiments using analogous block copolymers of hydrophobic SP monomer with a carbonate linkage (SPO) and conventional spiropyran methacrylate monomer (SPMA) containing a single ester functionality were then conducted, revealing that carbamate-incurred hydrogen bonding interactions in PEO-b-PSPA are crucial for polymersome stabilization in the zwitterionic MC state. Moreover, reversible phototriggered SP-to-MC polymersome transition is accompanied by membrane polarity and permeability switching from being nonimpermeable to selectively permeable toward noncharged, charged, and zwitterionic small molecule species below critical molar masses. Intriguingly, UV-actuated MC polymersomes possess two types of release modules: (1) sustained release upon short UV irradiation duration by taking advantage of the unexpectedly slow spontaneous MC-to-SP transition kinetics (t1/2 > 20 h) under dark conditions; (2) on-demand and switchable release under alternated UV-vis light irradiation. We further demonstrate photoswitchable spatiotemporal release of 4',6-diamidino-2-phenylindole (DAPI, cell nuclei-staining dye) within living HeLa cells.

Stimuli-triggered disassembly of block copolymer vesicles or polymersomes has been conventionally achieved via solubility switching of the bilayer-forming block, requiring cooperative changes of most of the repeating units. Herein we report an alternative approach by incorporating hydrophobic blocks exhibiting stimuli-triggered head-to-tail cascade depolymerization features. Amphiphilic block copolymers bearing this motif self-assemble into self-immolative polymersomes (SIPsomes). By modular design of terminal capping moieties, visible light, UV light, and reductive milieu can be utilized to actuate SIPsomes disintegration into water-soluble small molecules and hydrophilic blocks. The design of SIPsomes allows for triggered drug co-release and controllable access toward protons, oxygen, and enzymatic substrates. We also demonstrate programmed (OR-, AND-, and XOR-type logic) enzymatic reactions by integrating SIPsome encapsulation and trigger/capping moiety-selective cascade depolymerization events.