Biological responses according to the shape and size of carbon nanotubes in BEAS-2B and MESO-1 cells

Haniu, Hisao; Matsuda, Yoshikazu; Tsukahara, Tamotsu; Takanashi, Seiji; Maruyama, Kayo; Usui, Yuki; Aoki, Katsumi; Kobayashi, Shinsuke; Nomura, Hiroki; Tanaka, Manabu; Okamoto, Masato; Kato, Hiroyuki

doi:10.2147/ijn.s58661

Cited by 29 publications

(17 citation statements)

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“…We also found that CNHs were taken up into phagosomes. In our previous studies, we reported that different types of CNTs were incorporated into cells 31 and that the same CNTs affected the uptake into cells due to the dispersed state of particles. 21 In this study, like our previous study, we found that the aggregated CNT was incorporated into RAW264 cells, but that the amount of material taken up differed dramatically between highly dispersed FT9110 and CNHs showing the Notes: cultured raW264 cells were exposed to FT9110 at 10 µg/ml.…”

Section: Discussionmentioning

confidence: 99%

Different aggregation and shape characteristics of carbon materials affect biological responses in RAW264 cells

Kuroda

Ueda

Haniu

et al. 2018

IJN

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IntroductionCarbon nanotubes (CNTs) have various shapes, including needle-like shapes and curled shapes, and the cytotoxicity and carcinogenicity of CNTs differ depending on their shapes and surface modifications. However, the biological responses induced by CNTs and related mechanisms according to the dispersion state of CNTs have not been extensively studied.Materials and methodsWe prepared multiwalled CNTs (MWCNTs) showing different dispersions and evaluated these MWCNTs in RAW264 cells to determine cytotoxicity, cellular uptake, and immune responses. Furthermore, RAW264 cells were also used to compare the cellular uptake and cytotoxicity of fibrous MWCNTs and spherical carbon nanohorns (CNHs) exhibiting the same degree of dispersion.ResultsOur analysis showed that the cellular uptake, localization, and inflammatory responses of MWCNTs differed depending on the dispersion state. Moreover, there were differences in uptake between MWCNTs and CNHs, even showing the same degree of dispersion. These findings suggested that receptors related to cytotoxicity and immune responses differed depending on the aggregated state of MWCNTs and surface modification with a dispersant. Furthermore, our results suggested that the receptors recognized by the cells differed depending on the particle shape.ConclusionTherefore, to apply MWCNTs as a biomaterial, it is important to determine the carcinogenicity and toxicity of the CNTs and to examine different biological responses induced by varying shapes, dispersion states, and surface modifications of particles.

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Section: Discussionmentioning

confidence: 99%

Different aggregation and shape characteristics of carbon materials affect biological responses in RAW264 cells

Kuroda

Ueda

Haniu

et al. 2018

IJN

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“…However, the tangled-MWCNTs that have been investigated were not well dispersed, making it difficult to determine whether the results reflect the effects of nanosized, fibrous MWCNTs or the effects of microsized, agglomerated MWCNTs. We also reported that tangled-MWCNTs, named VGCF-X, showed significant cytotoxicity in the BEAS-2B human bronchial epithelial cell line but little cytotoxicity in the MESO-1 human malignant pleural mesothelioma cell line [18]. However, at that time we were not able to effectively disperse tangled VGCF-X.…”

Section: Introductionmentioning

confidence: 86%

The Dispersion State of Tangled Multi-Walled Carbon Nanotubes Affects Their Cytotoxicity

et al. 2016

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The medical applications of carbon nanotubes (CNTs) have garnered much attention. However, evaluating the safety of CNTs remains difficult, and no consensus has been reached. Moreover, assessing the biosafety of multi-walled CNTs (MWCNTs), which can become tangled during manufacturing, is challenging because they do not readily disperse. We studied how the dispersion state of tangled MWCNTs affects their cytotoxicity, using three sonicators. Flotube 9110 (FT9110), tangled MWCNTs, were dispersed in two dispersants (fetal bovine serum and polysorbate 80) using a new type of sonicator (PR-1) and two conventional sonicators. The size and cytotoxicity of the dispersed FT9110 were measured using the BEAS-2B human bronchial epithelial cell line. The PR-1 dispersed the FT9110 to agglomerates <200 nm in diameter; FT9110 dispersed with the PR-1 did not show cytotoxicity regardless of dispersant. The other sonicators dispersed the FT9110 to particles >1000 nm in diameter, and cytotoxicity depended on the dispersant. We found that excluding cells adhered to agglomerated FT9110 before evaluating cytotoxicity can lead to false-positive results. The PR-1 sonicator dispersed tangled FT9110 to many single fibers, which showed lower cytotoxicity than conventionally-sonicated MWCNTs. We suggest that dispersion state should be accounted for when evaluating the cytotoxicity of MWCNTs.

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“…CNTs diameter and length influence cell aggregation, ROS generation, and injury extent, the effects depending on the cell type too [117]. For example, long and thick rather than short and thin MWCNTs can induce inflammatory effects [118] as well as those with a larger diameter [119].…”

Section: The Impact Of Different Parameters Upon Cnt Cytotoxicitymentioning

confidence: 99%

In vivo Assessment of Nanomaterials Toxicity

Clichici¹,

Filip²

2015

Nanomaterials - Toxicity and Risk Assessment

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The concern regarding the safety of nanomaterials for the human body is constantly raising. On one hand, there is an increase in the production of nanomaterials for technological applications, which raises the risk of accidental exposure of the workers during the technological manipulation. On the other hand, nanomaterials can be designed for medical applications and their faith in the human body, along with their interactions with different tissues, becomes of vital importance.The mechanisms involved in nanomaterials toxicity are discussed, including oxidative stress and inflammation. In vivo toxicity evaluation includes different routes of administration or interaction between the nanomaterial and the organism, as well as a short-term or a long-term exposure or evaluation. "lso, the characteristics of nanomaterials, including size, shape, impurities, function, surface coating in relation to their toxicity, were discussed. " particular attention has been given to the evaluation of the toxicity of dendrimers, silver nanoparticles, gold nanoparticles and carbon nanotubes.Keywords: toxicity, oxidative stress, nanoparticles, carbon nanotubes . IntroductionResearch in the field of nanomaterials and nanotechnology has seen exponential growth in recent years due to multiple applications in different areas such as molecular imaging, drug delivery, engineering technology, and development of materials and medical devices for diagnosis and treatment. Generally, the biological effects of nanomaterials depend on their © 2015 The Author(s). Licensee InTech. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. electronic, magnetic, optical, and mechanical properties and also on their size, composition, and shape.However, the impact of these nanomaterials on human and environmental health is still limited. Most studies have investigated only the effects of unintended exposure inhalation, medical procedures or accidental ingestion and evaluated especially local effects, at the access site [ , ]. Nevertheless, due to the development of various biomedical procedures using nanomaterials it is necessary to understand their potential toxic effects at systemic level."n increasing number of studies published in the last decade have tried to decipher the complexity of interactions between different types of nanomaterials and cells. Nanomaterials usually get into the body either by inhalation and ingestion or transcutaneous [ , ], most often accidentally in the process of synthesis, through their usage, after wearing gold and silver jewelry or by eating food containing gold. Nevertheless, the behavior of nanoparticles inside the cells is still an enigma, and not all the metabolic and immunological responses induced by these particles are completely understood. In addition, the conclusions obtained have been variable and contrad...

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Biological responses according to the shape and size of carbon nanotubes in BEAS-2B and MESO-1 cells

Cited by 29 publications

References 47 publications

Different aggregation and shape characteristics of carbon materials affect biological responses in RAW264 cells

Different aggregation and shape characteristics of carbon materials affect biological responses in RAW264 cells

The Dispersion State of Tangled Multi-Walled Carbon Nanotubes Affects Their Cytotoxicity

In vivo Assessment of Nanomaterials Toxicity

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