FISH in micronucleus test demonstrates aneugenic action of rotenone in a common freshwater fish species, Nile tilapia (Oreochromis niloticus)

Abstract

Aneuploidies are numerical genetic alterations that lead to changes in the normal number of chromosomes due to abnormal segregation during cell division. This type of alteration can occur spontaneously or as a result of exposure to mutagenic agents. The presence of these agents in the environment has increased concern about potential damage to human health. Rotenone, derived from plants of the genera Derris and Lonchocarpus, is a product that is used all over the world as a pesticide and piscicide. Before establishing its potential and efficiency for these purposes, it is essential to know more about the possible adverse effects that it may cause. The current work aimed to evaluate the mutagenic potential of rotenone using fish from the species Oreochromis niloticus, as well as to help in understanding its action mechanism. Our results showed the mutagenic potential of rotenone evidenced by increased formation of micronuclei and nuclear buds at low doses of exposure. The use of fluorescence in situ hybridisation technique made it possible to measure the aneugenic potential of the substance, probably due to its impairment of mitotic spindle formation.

DNA damage markers in dermal fibroblasts in vitro reflect chronological donor age

“…We did not observe a substantial influence of rotenone on 53BP1 foci or TAF, which could be explained by its mostly aneugenic action [ 35 , 36 ]. As expected, stressing the fibroblasts with rotenone did results in higher numbers of micronuclei, but this higher prevalence of micronuclei in fibroblasts after rotenone incubation was also not associated with chronological donor age, similarly with previous results of human lymphocytes treated with irradiation [ 37 , 38 ] or mitomycin-C [ 39 ].…”

Genomic Organization Under Different Environmental Conditions:Hoplosternum Littoraleas a Model

“…[24][25][26] The micronucleus test (MN) [27][28][29] has been recommended for environmental biomonitoring studies, 17,30,31 mainly due to its capacity to qualitatively assess the effects of clastogenic and aneugenic agents. 16,[32][33][34][35] Fish have received special attention because they are efficient bioindicators of environmental quality and are capable of metabolizing xenobiotics and pollutants accumulated in the aquatic environment. [36][37][38][39] Additionally, they play different roles in the food chain and bioaccumulate contaminants dissolved in water.…”

Análise Do Polimorfismo De Deleção Dos Genes Gstm1 E Gstt1 E Diabetes Mellitus Em Idosos: Estudo Piloto

“…xenobióticas nos organismos (MELO et al, 2014;VENTURA et al, 2015;GOES et al, 2016), sendo eficiente nas respostas fisiológicas dos animais, além de representar um diagnóstico de sanidade que limita-se a extração de uma pequena quantidade de sangue, em que o animal pode ser devolvido em vida, sem danos, ao ambiente aquático em que habita.…”

“…Apresentam em sua parte ventral três pares de pernas chamadas de pernas anteriores, médias e posteriores (Figura 1D), constituídas por seis artículos, que são: coxa, trocanter, fêmur, tíbia, tarso e pré-tarso. As abelhas melíferas possuem a superfície externa da tíbia posterior em um formato côncavo com pelos nas margens, e tal arranjo é denominado de corbícula, estrutura onde o pólen é depositado para ser transportado (SILVEIRA; MELO;ALMEIDA, 2002).…”