Background: Fibromyalgia (FM) is a chronic pain syndrome characterized by generalized skeletal muscle chronic pain. Its etiology is not well defined, because there are several factors that may trigger it such as physical and/or emotional stresses, or a genetic susceptibility, involving serotonergic, dopaminergic and catecholaminergic paths. The objective of this study was to investigate the association between the strength of the lower limb, genetic polymorphism of the serotonin receptor gene HTR 2A in women with fibromyalgia. Methods: In this observational study of case-control type 48 women were evaluated who belonged to the group with FM (52 ± 12 years) and 100 women in the control group (58 ± 11 years). Socio demographic and anthropometric data were collected and peripheral blood samples for DNA extraction; genotypic analyzes were performed by means of PCR in real time by TaqMan® system. The lower limb muscle strength was assessed through the test of sitting down and standing up for 30 s. The chi-square test or Fischer Exact was used for possible associations among the variables; the t-test for independent samples was used to compare the averages among the groups; the value of significance adopted was 5%. Results: There was an association between the polymorphism of the HTR2A gene with FM, demonstrating that carriers of the genotype GG have 24.39 times more likely to develop the syndrome (IC95% 5.15-115.47; p = 0.01). It was observed an association between FM and the test to sit and stand up demonstrating that women with fibromyalgia have lower limb muscle strength (p = 0.01). The study showed that the white race has 3.84 times more likely to develop FM (p = 0.01). Conclusion: The results of this study suggest that women of Caucasian ethnicity with GG genotype or G allele presented greater risk of developing fibromyalgia and that these patients have lower limb muscle strength compared to the control group.

To investigate the influence of the catechol-O-methyltransferase enzyme (COMT) single nucleotide polymorphism (SNP) rs4680 (G/A) on fibromyalgia in women. In this observational study of case-control type 29 women with a diagnosis of FM (cases) and 31 healthy non-fibromyalgia women (controls). Sociodemographic and anthropometric data were collected, as well as data relating to Symptom Severity Scale and Generalized Pain Index and peripheral blood samples for DNA extraction; genotypic analyzes were performed by PCR-SSP. We observed that rs4680 AA genotype was more frequently observed in fibromyalgia than controls (p=0.02). The A allele was also more often present in the fibromyalgia participants than in their control peers (p = 0.03). There was a statistically significant association between race and FM sufferers, showing that those of white ethnicity had a 2.05 times greater chance of developing the syndrome than non-white individuals (p=0.03; CI 95% 0.93 – 4.53). A statistically significant correlation between age and FM was observed (rS=0.812, p=0.01). This study demonstrates that white women above the age of 45, who have the AA genotype or A-allele, presents a higher risk of developing FM, showing that this polymorphism of the COMT gene may be one of the risk factors for the fibromyalgia.

Este artigo relata sobre aspectos importantes no diagnóstico do hipotireoidismo em cães, visto que é uma enfermidade comum nesses animais, com apresentação de sinais clínicos bem característicos. Neste contexto, objetivou-se discutir sobre os principais métodos diagnósticos já que estes contribuem consequentemente para melhores tratamentos. Trata-se de uma revisão de literatura, realizada por meio de publicações de artigos científicos e livros obtidos em meios eletrônicos. O hipotireoidismo pode ser natural ou iatrogênico; e a partir daí faz-se tão necessário uma investigação precisa e correta. Além da realização de exames laboratoriais é essencial a avaliação dos aspectos dermatológicos, imunológicos e metabólicos no animal. Sugere-se, a ampliação do conhecimento sobre esses métodos bem como a capacitação dos profissionais para definição de prognósticos mais assertivos, visto que é essencial no contexto de diagnóstico dessa doença.

Introduction: Cardiovascular diseases (CVD) figure among the most significant causes of morbidity and mortality in the world and, among genetic factors, the literature has demonstrated the crucial role of miRNAs and the relationship of physical activity with this pathology. Objective: To investigate the relationship between the functional capacity of exercise, the level of physical activity, and the polymorphism in the miRNA-146a gene in elderly individuals with and without CVD. Methods: This study, developed in a city in the southern region of Brazil, is characterized as cross-sectional. The sample for this study comprised 342 participants, aged 60 or over. The following aspects were analyzed: anthropometric characteristics, genetic profiles, diagnosis of CVD, functional capacity, and the level of physical activity. Results: A statistically significant association was observed between CVD and body mass index (BMI) (א² = 14.278; p = 0.0003), and 40.6% of elderly individuals with CVD were obese, while 31.5% of the normally developed elderly participants presented normal BMI. However, the genotype frequencies (p = 0.546; א² = 1.211) and 6MWT (p = 0.311; א² = 1.025) did not show a statistically signifi-cant association with CVD. Conclusion: Our results suggest that the polymorphism in the miRNA-146A (rs2910164) and functional capacity are not associated with CVD in the elderly. However, the BMI did demonstrate an association with this disease.

The objective of this study was to verify the possible association between Glutathione Stransferase genetic polymorphism and functional physical aspects in the elderly with and without type 2 diabetes mellitus (DM2), as well as the increased susceptibility to its development. To carry out this cross-sectional study, 5 mL of blood was collected from 45 elderly people. The polymorphism was detected by the multiplex polymerase chain reaction (GSTM1 and GSTT1 polymorphisms). Functional physical aspects were assessed using physical tests. For statistical analysis, the Shapiro-and Qui Square tests ‫)²א(‬ were used. The presence of the GSTM1 and GSTT1 genes were more prevalent. There was a significant association between GSTT1 null genotypes and the presence / absence of DM2. No association was found between genotypes and functional physical aspects. It is concluded that the nullity of the GSTT1 genotype is associated with the susceptibility of DM2, but without a statistically significant relationship with functional physical aspects.

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