The effect of low-level laser therapy (LLLT) on the healing of skin lesions has been evaluated in many studies; however, the molecular mechanisms involved in the biostimulatory effects resulting from this treatment need to be better understood. The paper aims to analyze the effects of LLLT (660 nm) at doses of 1 and 5 J/cm on cell viability and expression of vascular endothelial growth factor (VEGF) and interleukin (IL6) genes in L929 fibroblast cells. The dose-response curve was performed with the GaInAlAs (660 nm) laser-treated cells at energy rates of 1 and 5 J/cm. Cell viability was quantified at 24, 48, and 72 h after irradiation and the effects of TLBP on the cytoskeleton and endoplasmic reticulum were evaluated by fluorescence microscopy and the RT-qPCR method was used for the analysis of gene expression. It was observed that the 72 h group had a statistically significant increase in cell viability compared to the 48 h group (p < 0.01) and when compared to the 72 h control (p = 0.03). In 72 h, a greater distribution of the cytoskeleton filaments and the more evident endoplasmatic reticulum was verified, indicating an increase in the protein synthesis when compared with the control group. In the expression of the VEGF gene, a significant increase of 1.98 times (p < 0.05) in the number of transcripts was observed; whereas for the IL6 gene, a decrease of the transcripts was 4.05 times (p < 0.05), both occurring within 72 h after irradiation at 5 J/cm. The LLLT (660 nm) at the dose of 5 J/cm should modulate cellular viability, upregulated VEGF, and downregulated IL6 expression of messenger RNA in culture of L929 fibroblast cells.
Introduction:In vitro and in vivo put in evidence that the Low Intensity Pulsed Ultrasound therapy exerts a significant influence on cell function (cytoskeleton organization, stimulation of mitochondrial activity, ATP levels and plasma membrane). Objective: This study will analyze the radiation of low intensity pulsed ultrasound in fibroblast cells L 929. Method: In this study are presented the data from each exposure group average and standard deviation in each moment of evaluation (24 hours, 48 hours and 72 hours). The control group (received no radiation), 0.2 W/cm2 with 10% pulse regime (1: 9 duty cycle), 0.2 W / cm2 with 20% pulse regime (2: 8 cycle work), 0.4 W/cm2 with pulse scheme 10% (1: 9 duty cycle), 0.4 W/cm2 with pulse scheme 20% (2: 8 duty cycle). The analyzes will be performed through optical microscopy, MTT method 3 -(4,5-dimethylthiazol-2-yl) -2,5 diphenyl tetrazolium bromide, within the incubation times of 24, 48 and 72 hours. Results: Given the above study, the results presented in this project will be directed to increase the stimulation process and proliferation of fibroblast cells from the pulsed ultrasonic irradiation of low intensity, correlating with the healing process, neovascularization and repair. Conclusion: Therefore, the study of the effect of ultrasound from cell culture provides us with a simple and informative model on the significant aspects of the use of physical therapy in vivo system.
Introduction: There is increasing interest from the general population, the search for the most suitable and safe exercise programs. Some equipment, such as Kangoo Jumps (KJ), allows performing exercises with less impact on the joints, and probably infer on postural control. Objective: To investigate the effect of exercise submaximal exercise running with and without the use of equipment KJ on postural control variables. Method: Participated in the study with an individual 20 years old, male, equivalent to 86kg of body weight. The test was to evaluate the postural balance using a force platform with bipedal before and after conventional and adapted from Cooper (using the KJ) test support. The equilibrium parameters were recorded: (A-COP in cm 2 ) of displacement of the center of pressure; average swing speed of the COP (MVeloc in cm/s) in the anteroposterior planes (A/P) and medial-lateral (M/L). Results: The results showed that after using the equipment KJ, A-COP (-39.70%) MVeloc the A/P (-20.66%) and M/L (-20.48%) were best compared with the race without using KJ test. Conclusion: It is concluded that after running exercise on submaximal exercise with the use of equipment KJ had become favorable outcome for postural balance. One such reason would be because of its system of absorption and dissipation of impact, which allows a better activation of the proprioceptive system through increasing the speed of afferent estimate. The intervention of this study shows that the use of the KJ has significant equipment to maintain postural stability near control values benefits. However, further studies are needed, due to lack of evidence of the parameters of this equipment and the small number in our sample. Keywords: exercise; postural balance; running. ResumoIntrodução: É crescente o interesse por parte da população em geral, pela busca de programas de exercícios físicos mais adequados e seguros. Alguns equipamentos, como o Kangoo Jumps (KJ), possibilitam a realização de exercícios com menor impacto sobre as articulações, além de provavelmente inferir sobre o controle postural. Objetivo: Verificar o efeito do exercício de esforço submáximo de corrida, com e sem a utilização do equipamento KJ, sobre variáveis do controle postural. Método: Participou do estudo um indivíduo com 20 anos de idade, sexo masculino, peso corporal equivalente a 86 kg. O teste consistiu em avaliar o Equilíbrio Postural utilizando uma plataforma de força com apoio bipodal, pré e pós teste de Cooper convencional e adaptado (com o uso do KJ). Os parâmetros de equilíbrio registrados foram: área de deslocamento do centro de pressão (A-COP em cm 2 ); velocidade média de oscilação do COP (MVeloc em cm/s) nos planos ântero-posterior (A/P) e médio-lateral (M/L). Resultados: Os resultados demonstraram, que após o uso do equipamento KJ, a A-COP (-39,70%), a MVeloc A/P (-20,66%) e M/L (-20,48%) foram melhores, quando comparadas com o teste de corrida sem o uso do KJ. Conclusão: Conclui-se que após o exercício de corrida em esforço submáximo, com a ut...
Introduction: The ultrasound is widely used clinical practice focused on tissue repair, because it is a secure resource, minimally-invasive and low investment. However, despite the effects of ultrasound have been well described, the ideal relationship between dose and effect, still needs to be better elucidated and bounded. Objective: In this way, the objective of this study was to evaluate the influence of irradiation pulsed ultrasound and continuous in the viability of fibroblastic cells L929 in vitro. Method: The cells were distributed in TPP plates 12 wells in the concentration of 5x10 5 cells/µL and subdivided into the following groups: G1: control (not received radiation), G2: irradiated to 0.5 W/cm 2-30% and G3: irradiated to 0.5 W/cm 2-100%. Then received ultrasonic irradiation with intervals of 24, 48 and 72 hours, and after 24 hours of each irradiation was performed MTT cytotoxicity cell. Results: Among the three groups analyzed, only the G2 group showed a significant difference between the time 48 and 72 hours (p=0.05). In other times, despite variation in the percentage of viable cells, were not significant. When compared to peers at post-test, it was possible to observe a difference of 29% of viable cells between the groups G1 and G2 (p=0.05). However, the biggest difference was observed between the groups G2 and G3 (p=0.05). Conclusion: In this way, it can be concluded that irradiation pulsed ultrasound showed higher proliferation of fibroblasts L929 in time 48 hours, whereas in group irradiated in continuous mode, there was no statistically significant difference between the times.
To investigate the influence of the catechol-O-methyltransferase enzyme (COMT) single nucleotide polymorphism (SNP) rs4680 (G/A) on fibromyalgia in women. In this observational study of case-control type 29 women with a diagnosis of FM (cases) and 31 healthy non-fibromyalgia women (controls). Sociodemographic and anthropometric data were collected, as well as data relating to Symptom Severity Scale and Generalized Pain Index and peripheral blood samples for DNA extraction; genotypic analyzes were performed by PCR-SSP. We observed that rs4680 AA genotype was more frequently observed in fibromyalgia than controls (p=0.02). The A allele was also more often present in the fibromyalgia participants than in their control peers (p = 0.03). There was a statistically significant association between race and FM sufferers, showing that those of white ethnicity had a 2.05 times greater chance of developing the syndrome than non-white individuals (p=0.03; CI 95% 0.93 – 4.53). A statistically significant correlation between age and FM was observed (rS=0.812, p=0.01). This study demonstrates that white women above the age of 45, who have the AA genotype or A-allele, presents a higher risk of developing FM, showing that this polymorphism of the COMT gene may be one of the risk factors for the fibromyalgia.
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